Current Science

Open Access Open Access  Restricted Access Subscription Access

In vitro Propagation of Some Threatened Plant Species of India


Affiliations
1 Department of Botany, Nagaland University, Lumami 798 627, India
2 Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar 751 003, India
3 Botanical Survey of India, Eastern Regional Centre, Shillong 793 003, India
4 G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, India
 

To prevent extinction of threatened species, in vitro regeneration protocols for the propagation of six threatened species were standardized. The regenerated micro-shoots were ischolar_mained in nutrient medium supplemented with low concentrations of auxin. The well-developed plantlets were successfully established in field conditions, thus improving the probability of self-sustenance of the introduced populations. The success story of these six threatened species reaffirms the role of in vitro propagation in conserving plants facing the threat of extinction.

Keywords

Conservation, In vitro Regeneration, Microshoots, Threatened Species.
User
Notifications
Font Size

  • MoEF, India’s 5th National report to the conservation on biological diversity. Ministry of Environment and Forest, Government of India, 2015, p. 142.

  • Cruz-Cruz, C. A., Gonzalez-Arnao, M. A. and Engelmann, F., Biotechnology and conservation of plant biodiversity. Resources, 2013, 2, 73–95.

  • Langhu, T. and Deb. C. R., Studies on the reproductive biology and seed biology of Aconitum nagarum Stapf: a threatened medicinal plant of North East India. J. Res. Biol., 2014, 4(7), 1465–1474.

  • Engelmann, F., Germplasm collection, storage and conservation. In Plant Biotechnology and Agriculture (eds Altman, A. and Hasegawa, P. M.), Academic Press, Oxford, UK, 2012, pp. 255–268.

  • Withers, L. A., Collecting in vitro for genetic resources conservation. In Collecting Plant Genetic Diversity (eds. Guarino, L., Rao, R. and Reid, R.), Centre for Agricultural Bioscience International, Wallingford, UK, 1995, pp. 511–515.

  • Engelmann, F., Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell. Dev. Biol.–Plant, 2011, 47, 5–16.

  • Arenmongla, T. and Deb, C. R., Studies on in vitro morphogenetic potential of nodal segments of Malaxis acuminate D. Don. Appl. Biol. Res., 2012, 14(2), 156–163.

  • Deb, C. R. and Arenmongla, T., An efficient in vitro regeneration protocol for a natural dye yielding plant, Strobilanthes flaccidifolious Nees. from nodal explants. Indian J. Exp. Biol., 2012, 50, 810–816.

  • Deb, M. S., Jamir, N. S. and Deb, C. R., In vitro culture of immature embryos of Cinnamomum tamala Nees. – the role of different factors. Indian J. Exp. Biol., 2014, 52, 1003–2010.

  • Tandon, P. and Kumaria, S., Prospects of plant conservation biotechnology in India with special reference to north-eastern region. In Biodiversity: Status and Prospects (eds Tandon, P. and Kumaria, S.), Narosa Publishing House, New Delhi, 2005, pp. 79–91.

  • Sharma, B. D. and Sanjappa, M., Flora of India, Botanical Survey of India, Kolkata, India, 1993, vol. 3, pp. 142–145.

  • Samant, S. S., Diversity, nativity and endemism of vascular plants in a part of Nanda Devi Biosphere Reserve in West Himalaya I. Himalaya Biosphere Reserve (Biannual Bull.), 1999, 1(1–2), 1–28.

  • Ernst, E., St John’s wort, an anti-depressant? a systematic, criteriabased review. Phytomedicine, 1995, 2, 67–71.

  • Swain, D., Lenka, S., Hota, T. and Rout, G. R., Micro-propagation of Hypericum gaitii Haines, an endangered medicinal plants: assessment of genetic fidelity. Nucleus, 2016, 59(1), 7–13.

  • Ved, D. K., Kinhal, G. A., Ravikumar, K., Prabhakaran, V., Ghate, U., Vijaya Sankar, R. and Indresha, J. H., Conservation assessment and management prioritization for the medicinal plants of Himachal Pradesh, Jammu & Kashmir and Uttaranchal. Foundation of Revitalization of Local Health Traditions, Bengaluru, 2003, pp. 1–24.

  • Van Uden, W., Pras, N., Visser, J. F. and Malingre, T. M., Detection and identification of podophyllotoxin produced by cell cultures derived from Podophyllum hexandrum Royle. Plant Cell Rep., 1989, 8, 165–168.

  • Canel, C., Moraes, R. M., Dyan, F. E. and Ferreira, D., Molecules of interest: podophyllotoxin. Photochemistry, 2000, 54, 115–120.

  • Nadeem, M., Palni, L. M. S., Purohit, A. N., Pandey, H. and Nandi, S. K., Propagation and conservation of Podophyllum hexandrum Royle: an important medicinal herb. Biol. Conserv., 2000, 92, 121–129.

  • Deb, C. R. and Imchen, T., An efficient in vitro hardening technique of tissue culture raised plants. Biotechnology, 2010, 9(1), 79–83.

  • Mao, A. A. and Gogoi, R., Rhododendrons of Manipur and Nagaland, India. NeBIO, 2012, 3(1), 1–10.

  • Wu, F. Q., Shen, S. K., Zhang, X. J., Wang, Y. H. and Sun, W. B., Genetic diversity and population structure of an extremely endangered species: the world’s largest Rhododendron. AoB PLANTS, 2015, 7, plu082; doi:10.1093/aobpla/plu082.

  • Santos, A., Fidalgo, F., Santos, I. and Salema, R., In vitro bulb formation of Narcissus asturiensis, a threatened species of the Amaryllidaceae. J. Hortic. Sci. Biotechnol., 2002, 77, 149–152.

  • Kyte, L. and Briggs, B., A simplified entry into tissue culture production of rhododendrons. Proc. Int. Plant Propaga. Soc., 1979, 29, 90–95.

  • McCown, B. H. and Lloyd, C. B., A survey of the response of Rhododendron to in vitro culture. Plant Cell Tissue Organ Cult., 1982, 2, 77–85.

  • De, A. and Hajra, P. K., Vanda in India. J. Orchid Soc. India, 2004, 18, 28–29.

  • Karuppusamy, S., Aruna, V., Kiranmai, C. and Pullaiah, T., In vitro propagation of an endemic umbellifer, Hydrocotyle conferta. Indian J. Biotechnol., 2007, 6, 541–544.

  • Dhavala, A. and Rathore, T. S., Micropropagation of Embelia ribes Burm f. through proliferation of adult axillary shoots. In Vitro Cell. Dev. Biol.–Plant., 2010, 46, 180–191.

  • Mangal, M., Sharma, D., Sharma, M., Kher, R. and Singh, A. K., In vitro plantlet regeneration in Guava from nodal segments. Phytomorphology, 2008, 58, 103–108.

  • Abhyankar, G. and Reddy, V. D., Rapid micropropagation via axillary bud proliferation of Adhatoda vasica Nees from nodal segments. Indian J. Exp. Biol., 2007, 45, 268–271.

  • Liu, Z. R. and Sandford, J. C., Plant regeneration by organogenesis from strawberry leaf and runner tissue. Hortic. Sci., 1988, 23, 1057–1059.

  • Rout, G. R., Mahato, A. and Senapati, S. K., In vitro clonal propagation of Nyctanthes arbortristis. Biol. Plant., 2008, 52, 521–524.

  • Rout, G. R., Direct plant regeneration from leaf explants of Plumbago species and its genetic fidelity through RAPD markers. Ann. Appl. Biol., 2003, 140, 305–313.

  • Calvo, M. C. and Segura, J., Plant regulation from cultured leaves of Lavandula latifolia Medicus: Influences of growth regulators and illumination condition. Plant Cell Tissue Organ Cult., 1989, 19, 33–42.

  • Baraldi, R., Rossi, F. and Lercari, B., In vitro shoot development of Prunus GF 6652: interaction between light and benzyladenine. Plant Physiol., 1988, 74, 440–443.

  • Cantos, M., Linan, J., Garcia, J. L., Linan, M. G., Dominguez, M. A. and Troncoso, A., The use of in vitro culture to improve the propagation of Rhododendron ponticum sub sp. baeticum (Boiss. & Reuter). Cent. Eur. J. Biol., 2007, 2, 297–306.

  • Selvaraj, N., Vasudevan, A., Manickavasagam, M. and Ganapathi, A., In vitro organogenesis and plant formation in cucumber. Biol. Plant., 2006, 50, 123–126.

  • Baskaran, P., Velayutham, P. and Jayabalan, N., In vitro regeneration of Melothriamadera spatana via indirect organogenesis. In Vitro Cell. Dev. Biol.–Plant., 2009, 45, 407–413.

  • Manzanera, J. A. and Pardos, J. A., Micropropagation of juvenile and adult Quercus suber L. Plant Cell Tissue Organ Cult., 1990, 21, 1–8.


Abstract Views: 346

PDF Views: 152




  • In vitro Propagation of Some Threatened Plant Species of India

Abstract Views: 346  |  PDF Views: 152

Authors

C. R. Deb
Department of Botany, Nagaland University, Lumami 798 627, India
G. R. Rout
Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar 751 003, India
A. A. Mao
Botanical Survey of India, Eastern Regional Centre, Shillong 793 003, India
S. K. Nandi
G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, India
R. K. Nilasana Singha
Botanical Survey of India, Eastern Regional Centre, Shillong 793 003, India
D. Vijayan
Botanical Survey of India, Eastern Regional Centre, Shillong 793 003, India
T. Langhu
Department of Botany, Nagaland University, Lumami 798 627, India
Z. P. Kikon
Department of Botany, Nagaland University, Lumami 798 627, India
S. Pradhan
Botanical Survey of India, Eastern Regional Centre, Shillong 793 003, India
Mohd Tariq
G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, India
D. Swain
Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar 751 003, India

Abstract


To prevent extinction of threatened species, in vitro regeneration protocols for the propagation of six threatened species were standardized. The regenerated micro-shoots were ischolar_mained in nutrient medium supplemented with low concentrations of auxin. The well-developed plantlets were successfully established in field conditions, thus improving the probability of self-sustenance of the introduced populations. The success story of these six threatened species reaffirms the role of in vitro propagation in conserving plants facing the threat of extinction.

Keywords


Conservation, In vitro Regeneration, Microshoots, Threatened Species.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi03%2F567-575