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Identification of Bovine Viral Diarrhoea Virus Type 2 in Cattle Bull Semen from Southern India and its Genetic Characterization


Affiliations
1 ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
2 Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India
 

Although bovine viral diarrhoea virus (BVDV) is prevalent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n = 4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from southern India by real-time RT-PCR. Genetic typing of the 5'-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found genetically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences confirmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India highlights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.

Keywords

Bovine Viral Diarrhoea, Bull Semen, Genetic Characterization, Mandatory Testing.
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  • Identification of Bovine Viral Diarrhoea Virus Type 2 in Cattle Bull Semen from Southern India and its Genetic Characterization

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Authors

Niranjan Mishra
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
Semmannan Kalaiyarasu
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
K. C. Mallinath
Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India
Katherukamem Rajukumar
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
Rohit K. Khetan
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
Siddharth Gautam
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
M. D. Venkatesha
Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India
S. M. Byregowda
Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India

Abstract


Although bovine viral diarrhoea virus (BVDV) is prevalent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n = 4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from southern India by real-time RT-PCR. Genetic typing of the 5'-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found genetically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences confirmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India highlights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.

Keywords


Bovine Viral Diarrhoea, Bull Semen, Genetic Characterization, Mandatory Testing.

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DOI: https://doi.org/10.18520/cs%2Fv114%2Fi03%2F666-670