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Identification of Bovine Viral Diarrhoea Virus Type 2 in Cattle Bull Semen from Southern India and its Genetic Characterization


Affiliations
1 ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
2 Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India
 

Although bovine viral diarrhoea virus (BVDV) is prevalent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n = 4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from southern India by real-time RT-PCR. Genetic typing of the 5'-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found genetically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences confirmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India highlights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.

Keywords

Bovine Viral Diarrhoea, Bull Semen, Genetic Characterization, Mandatory Testing.
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  • King, A. M. Q., Adams, M. J., Carstens, E. B. and Lefkowitz, E. J., Virus taxonomy: classification and nomenclature of viruses. In Ninth Report of the International Committee on Taxonomy of Viruses, Academic Press, San Diego, CA, USA, 2011, pp. 1003–1020.

  • Meyers, G. and Thiel, H. J., Molecular characterization of pestiviruses. Adv. Virus Res., 1996, 47, 53–118.

  • Vilcek, S. et al., Bovine viral diarrhoea virus genotype 1 can be separated into at least eleven genetic groups. Arch. Virol., 2001, 146, 99–115.

  • Becher, P. et al., Genetic and antigenic characterization of novel pestivirus genotypes: implication for classification. Virology, 2003, 311, 96–104.

  • Mishra, N. et al., Identification and molecular characterization of novel and divergent HoBi-like pestiviruses from naturally infected cattle in India. Vet. Microbiol., 2014, 174, 239–246.

  • Niskanen, R. and Lindberg, A., Transmission of bovine viral diarrhoea virus by unhygienic vaccination procedures, ambient air, and from contaminated pens. Vet. J., 2003, 165, 125–130.

  • Kirkland, P. D., Richards, S. G., Rothwell, J. T. and Stanley, D. F., Replication of bovine viral diarrhoea virus in the bovine reproductive tract and excretion of virus in semen during acute and chronic infections. Vet. Rec., 1991, 128, 587–590.

  • Grooms, D. L., Reproductive consequences of infection with bovine viral diarrhoea virus. Vet. Clin. North Am. Food Anim. Pract., 2004, 20, 5–19.

  • Kirkland, P. D., MacIntosh, S. G. and Mole, A., The outcome of widespread use of semen from a bull persistently infected with pestivirus. Vet. Rec., 1994, 135, 527–529.

  • Voges, H., Horner, G. W., Rowe, S. and Wleenberg, G. J., Persistent bovine pestivirus infection localized in the testes of an immunocompetent, non-viremic bull. Vet. Microbiol., 1998, 61, 165–175.

  • Givens, M. D. et al., Noncytopathic bovine viral diarrhoea virus can persist in testicular tissue after vaccination of peri-pubertal bulls but prevents subsequent infection. Vaccine, 2007, 25, 867–876.

  • Mishra, N. et al., Genetic typing of bovine viral diarrhoea virus isolates from India. Vet. Microbiol., 2004, 104, 207–212.

  • Behera, S. P. et al., Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India. Comp. Immunol. Microbiol. Infect. Dis., 2011, 34, 189–196.

  • OIE, Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis. In Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Office International des Epizooties, Paris, 2016, chapter 2.4.7, pp. 1–22.

  • Hoffmann, B., Depner, K., Schirrmeier, H. and Beer, M., A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. Virol. Methods, 2006, 136, 200–209.

  • Vilcek, S., Herring, A. J., Herring, J. A., Nettleton, P. F., Lowings, J. P. and Paton, D. J., Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch. Virol., 1994, 136, 309–323.

  • Gaede, W., Reiting, R., Schirrmeier, H., Depner, K. R. and Beer, M., Detection and species-specific differentiation of pestiviruses using real-time RT-PCR. Berl. Muench. TierarztlWochenschr, 2005, 118, 113–120.

  • Baxi, M., McRae, D., Baxi, S., Greiser-Wilke, I., Vilcek, S., Amoako, K. and Deregt, D., A one-step multiplex real time RT-PCR for detection and typing of bovine viral diarrhoea viruses. Vet. Microbiol., 2006, 116, 37–44.

  • Liu, L., Xia, H., Belak, S. and Baule, C., A TaqMan real-time RT-PCR assay for selective detection of atypical bovine pestiviruses in clinical samples and biological products. J. Virol. Methods, 2008, 154, 82–85.

  • Nagai, M. et al., Phylogenetic analysis of bovine viral diarrhoea viruses using five different genetic regions. Virus Res., 2004, 99, 103–113.

  • Becher, P., Orlich, M., Shannon, A. D., Horner, G., Konig, M. and Thiel, H. J., Phylogenetic analysis of pestiviruses from domestic and wild ruminants. J. Gen. Virol., 1997, 78, 1357–1366.

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S., MEGA6: Molecular evolutionary genetics analysis version 6.0, Mol. Biol. Evol., 2013, 30, 2725–2729.

  • Gonzalez Altamiranda, E. A. et al., Clinical and reproductive consequences of using BVDV-contaminated semen in artificial insemination in a beef herd in Argentina. Anim. Rep. Sci., 2012, 133, 146–152.

  • Givens, M. D., Heath, A. M., Brock, K. V., Brodersen, B. W., Carson, R. L. and Stringfellow, D. A., Detection of bovine viral diarrhoea virus in semen obtained after inoculation of seronegative postpubertal bulls. Am. J. Vet. Res., 2003, 64, 428–434.

  • Mishra, N., Rajukumar, K., Vilcek, S., Tiwari, A., Satav, J. S. and Dubey S. C., Molecular characterization of bovine viral diarrhoea virus type 2 isolate originating from a native Indian sheep (Ovis aries). Vet. Microbiol., 2008, 130, 88–98.

  • Jenckel, M., Höper, D., Schirrmeier, H., Reimann, I., Goller, K. V., Hoffmann, B. and Beer, M., Mixed triple: allied viruses in unique recent isolates of highly virulent type 2 bovine viral diarrhoea virus detected by deep sequencing. J. Virol., 2014, 88, 6983–6992.

  • Workman, A. M. et al., Resolving bovine viral diarrhoea virus subtypes from persistently infected U.S. beef calves with complete genome. J. Vet. Diagn. Invest., 2016, 28, 519–528.

  • Mishra, N., Dubey, R., Rajukumar, K., Tosh, C., Tiwari, A. and Pitale, S. S., Genetic and antigenic characterization of Indian bovine viral diarrhoea virus type 2 isolated from Indian goats (Capra hircus). Vet. Microbiol., 2007, 124, 340–347.


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  • Identification of Bovine Viral Diarrhoea Virus Type 2 in Cattle Bull Semen from Southern India and its Genetic Characterization

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Authors

Niranjan Mishra
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
Semmannan Kalaiyarasu
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
K. C. Mallinath
Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India
Katherukamem Rajukumar
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
Rohit K. Khetan
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
Siddharth Gautam
ICAR-National Institute of High Security Animal Diseases, Anand Nagar, Bhopal 462 022, India
M. D. Venkatesha
Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India
S. M. Byregowda
Southern Regional Disease Diagnostic Laboratory, Institute of Animal Health and Biologicals, Hebbal, Bengaluru 560 024, India

Abstract


Although bovine viral diarrhoea virus (BVDV) is prevalent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n = 4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from southern India by real-time RT-PCR. Genetic typing of the 5'-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found genetically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of Npro sequences confirmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India highlights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.

Keywords


Bovine Viral Diarrhoea, Bull Semen, Genetic Characterization, Mandatory Testing.

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DOI: https://doi.org/10.18520/cs%2Fv114%2Fi03%2F666-670