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Time Reduction for Determination of Infective Propagule Numbers of Arbuscular Mycorrhizal Fungi by Most Probable Number Assay


Affiliations
1 Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
 

It is now well documented that arbuscular mycorrhizal fungi (AMF) improve fitness and growth of plants which are important in agriculture, horticulture and forestry. An important task in most AM studies is to determine accurately the number of infective propagules (IP) of AMF in soil, substrate, or inoculum. The most probable number (MPN) technique (or method of ultimate dilution) for enumerating viable microorganisms, is a possible solution to the problems faced when using the usual methods of counting AMF endophyte propagules.
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  • Time Reduction for Determination of Infective Propagule Numbers of Arbuscular Mycorrhizal Fungi by Most Probable Number Assay

Abstract Views: 418  |  PDF Views: 149

Authors

T. Abinaya
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
D. J. Bagyaraj
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
G. Thilagar
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
R. Ashwin
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India

Abstract


It is now well documented that arbuscular mycorrhizal fungi (AMF) improve fitness and growth of plants which are important in agriculture, horticulture and forestry. An important task in most AM studies is to determine accurately the number of infective propagules (IP) of AMF in soil, substrate, or inoculum. The most probable number (MPN) technique (or method of ultimate dilution) for enumerating viable microorganisms, is a possible solution to the problems faced when using the usual methods of counting AMF endophyte propagules.

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DOI: https://doi.org/10.18520/cs%2Fv114%2Fi04%2F729-730