Current Science

Open Access Open Access  Restricted Access Subscription Access

Time Reduction for Determination of Infective Propagule Numbers of Arbuscular Mycorrhizal Fungi by Most Probable Number Assay


Affiliations
1 Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
 

It is now well documented that arbuscular mycorrhizal fungi (AMF) improve fitness and growth of plants which are important in agriculture, horticulture and forestry. An important task in most AM studies is to determine accurately the number of infective propagules (IP) of AMF in soil, substrate, or inoculum. The most probable number (MPN) technique (or method of ultimate dilution) for enumerating viable microorganisms, is a possible solution to the problems faced when using the usual methods of counting AMF endophyte propagules.
User
Notifications
Font Size

  • Bagyaraj, D. J., Sharma, M. P. and Maiti, D., Curr. Sci., 2015, 108, 1288–1293.

  • Porter, W. M., Aust. J. Soil Res., 1979, 17, 515–519.

  • Philips, J. M. and Hayman, D. S., Trans. Br. Mycol. Soc., 1970, 55, 158–161.

  • Alexander, M., In Methods of Soil Analysis, Part 2, Chemical and Microbiological Properties (ed. Black, C. A.), American Society of Agronomy: Madison, Wisconsin, USA, 1965, pp. 1467–1472.

  • Harinikumar, K. M. and Bagyaraj, D. J., J. Soil Biol. Ecol., 1992, 12, 91–94.

  • The Fertilizer (Control) Order 1985 (as amended up to April 2015), The Fertilizer Association of India, New Delhi, 1985, p. 181.


Abstract Views: 402

PDF Views: 140




  • Time Reduction for Determination of Infective Propagule Numbers of Arbuscular Mycorrhizal Fungi by Most Probable Number Assay

Abstract Views: 402  |  PDF Views: 140

Authors

T. Abinaya
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
D. J. Bagyaraj
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
G. Thilagar
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India
R. Ashwin
Centre for Natural Biological Resources and Community Development, 41 RBI Colony, Anand Nagar, Bengaluru 560 024, India

Abstract


It is now well documented that arbuscular mycorrhizal fungi (AMF) improve fitness and growth of plants which are important in agriculture, horticulture and forestry. An important task in most AM studies is to determine accurately the number of infective propagules (IP) of AMF in soil, substrate, or inoculum. The most probable number (MPN) technique (or method of ultimate dilution) for enumerating viable microorganisms, is a possible solution to the problems faced when using the usual methods of counting AMF endophyte propagules.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi04%2F729-730