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Phosphate Solubilization Mechanisms in Alkaliphilic Bacterium Bacillus marisflavi FA7


Affiliations
1 Department of Microbiology, Goa University, Panjim 403 206, India
2 Department of Microbiology, P.E.S’s Shri Ravi S. Naik College of Arts and Science, Ponda 403 401, India
 

This study reports the mechanisms of phosphate solubilization present in alkaliphilic Bacillus marisflavi FA7. The strain obtained from sediment samples of mangrove ecosystem exhibited different mechanisms to solubilize inorganic phosphate and mineralize organic phosphate under alkaline conditions. It reduced the pH of the medium that showed near perfect correlation with tri-calcium phosphate solubilization. Organic acids produced by the strain were detected in broth. Maximum decrease in pH of the medium was observed with NH4Cl as an inorganic nitrogen source. This indicated involvement of proton extrusion mechanism toward phosphate solubilization during ammonium uptake. It produced exopolysaccharide, but failed to produce siderophore. Bacillus marisflavi FA7 produced extracellular alkaline phosphatase having molecular weight of 175–200 kDa. The pH optimum for maximum enzyme activity was 10.1 and Km of 1.13 μM p-nitrophenolphosphate. This is the first study to report the highest tri-calcium phosphate solubilization by an alkaliphilic bacterium.

Keywords

Alkaliphilic Bacterium, Bacillus, Mechanism, Phosphate Solubilization, Phosphatase.
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  • Phosphate Solubilization Mechanisms in Alkaliphilic Bacterium Bacillus marisflavi FA7

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Authors

Neha Prabhu
Department of Microbiology, Goa University, Panjim 403 206, India
Sunita Borkar
Department of Microbiology, P.E.S’s Shri Ravi S. Naik College of Arts and Science, Ponda 403 401, India
Sandeep Garg
Department of Microbiology, Goa University, Panjim 403 206, India

Abstract


This study reports the mechanisms of phosphate solubilization present in alkaliphilic Bacillus marisflavi FA7. The strain obtained from sediment samples of mangrove ecosystem exhibited different mechanisms to solubilize inorganic phosphate and mineralize organic phosphate under alkaline conditions. It reduced the pH of the medium that showed near perfect correlation with tri-calcium phosphate solubilization. Organic acids produced by the strain were detected in broth. Maximum decrease in pH of the medium was observed with NH4Cl as an inorganic nitrogen source. This indicated involvement of proton extrusion mechanism toward phosphate solubilization during ammonium uptake. It produced exopolysaccharide, but failed to produce siderophore. Bacillus marisflavi FA7 produced extracellular alkaline phosphatase having molecular weight of 175–200 kDa. The pH optimum for maximum enzyme activity was 10.1 and Km of 1.13 μM p-nitrophenolphosphate. This is the first study to report the highest tri-calcium phosphate solubilization by an alkaliphilic bacterium.

Keywords


Alkaliphilic Bacterium, Bacillus, Mechanism, Phosphate Solubilization, Phosphatase.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi04%2F845-853