Current Science

Open Access Open Access  Restricted Access Subscription Access

Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels


Affiliations
1 Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
2 Department of Life Science, Heze University, Heze, Shandong 274015, China
 

To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5–5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.

Keywords

Biofuels, Metabolic Engineering, Plant Oil, Tobacco Leaves, VgDGAT1a Gene.
User
Notifications
Font Size

  • Barthole, G., Lepiniec, L., Rogowsky, P. M. and Baud, S., Controlling lipid accumulation in cereal grains. Plant Sci.: Int. J. Exp. Plant Biol., 2012, 185–186(4), 33–39.

  • Ohlrogge, J. B. and Jaworski, J. G., Regulation of fatty acid synthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997, 4, 109–136.

  • Baud, S. and Lepiniec, L., Physiological and developmental regulation of seed oil production. Prog. Lipid Res., 2010, 3, 235–249.

  • Guschina, I. A., Everard, J. D., Kinney, A. J., Quant, P. A. and Harwood, J. L., Studies on the regulation of lipid biosynthesis in plants: application of control analysis to soybean. Biochim. Biophys. Acta, 2014, 6, 1488–1500.

  • Tajima, D. et al., Wrinkled 1 (WRI1) homologs, AP2-type transcription factors involving master regulation of seed storage oil synthesis in castor bean (Ricinus communis L.). Am. J. Plant Sci., 2013, 2, 333–339.

  • Wu, X.-L., Liu, Z.-H., Hu, Z.-H. and Huang, R.-Z., BnWRI1 coordinates fatty acid biosynthesis and photosynthesis pathways during oil accumulation in rapeseed. J. Int. Plant Biol., 2014, 6, 582–593.

  • Li, R., Yu, K., Hatanaka, T. and Hildebrand, D. F., Vernonia DGATs increase accumulation of epoxy fatty acids in oil. Plant Biotechnol. J., 2010, 2, 184–195.

  • Yu, K., Li, R., Hatanaka, T. and Hildebrand, D., Cloning and functional analysis of two type 1 diacylglycerol acyltransferases from Vernonia galamensis. Phytochemistry, 2008, 5, 1119–1127.

  • Li, R., Yu, K. and Hildebrand, D. F., DGAT1, DGAT2 and PDAT expression in seeds and other tissues of epoxy and hydroxy fatty acid accumulating plants. Lipids, 2010, 45, 145–157.

  • Zhang, F.-Y., Yang, M.-F. and Xu, Y.-N., Silencing of DGAT1 in tobacco causes a reduction in seed oil content. Plant Sci., 2005, 4, 689–694.

  • Jako, C. et al., Seed-specific overexpression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. Biochem.: PPB/Soc. Fran. Phys. Veg., 2001, 126, 861–874.

  • Weselake, R. J. et al., Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content. J. Exp. Bot., 2008, 59, 3543–3549.

  • Shao, S. and Hegde, R. S., Membrane protein insertion at the endoplasmic reticulum. Annu. Rev. Cell. Dev. Biol., 2011, 27, 25–56.

  • Cramer, C. L. et al., Bioproduction of human enzymes in transgenic tobacco. Ann. N.Y. Acad. Sci., 1996, 25, 62–71.

  • Voinnet, O., Rivas, S., Mestre, P. and Baulcombe, D., An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J.: Cell Mol. Biol., 2003, 5, 949–956.

  • Youa, C.-X. et al., Chemical constituents and biological activities of the Purple Perilla essential oil against Lasioderma serricorne. Ind. Crops Prod., 2014, 61, 331–337.

  • Grayburn, W. S., Collins, G. B. and Hildebrand, D. F., Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue. Bio/Technol., 1992, 6, 675–678.

  • Bouvier-Nave, P., Benveniste, P., Oelkers, P., Sturley, S. L. and Schaller, H., Expression in yeast and tobacco of plant cDNAs encoding acyl CoA: diacylglycerol acyltransferase. Eur. J. Bio-chem., 2000, 1, 85–96.

  • Vanhercke, T., El Tahchy, A., Shrestha, P., Zhou, X. R., Singh, S. P. and Petrie, J. R., Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants. FEBS Lett., 2013, 4, 364–369.

  • Livak, K. J. and Schmittgen, T. D., Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 2001, 4, 402–408.

  • Dahmer, M. L., Fleming, P. D., Collins, G. B. and Hildebrand, D. F., A rapid screening technique for determining the lipid composition of soybean seeds. J. Am. Oil Chem. Soc., 1989, 4, 543–548.

  • Andrianov, V. et al., Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. Plant Biotechnol. J., 2010, 3, 277–287.

  • Hanying, W., Chao, L., Minchun, L., Mingming, Z., Dan, G. and Yinong, X., Effects of monogalactoglycerolipid deficiency and diacylglycerol acyltransferase overexpression on oil accumulation in transgenic tobacco. Plant Mol. Biol. Rep., 2013, 5, 1077–1088.

  • Li, R., Yua, K., Wu, Y., Tateno, M., Hatanaka, T. and Hildebrand, D. F., Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds. Metab. Eng., 2012, 1, 29–38.

  • Lardizabal, K. et al., Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean. Plant Physiol., 2008, 148, 89–96.

  • Sharma, N. et al., Transgenic increases in seed oil content are associated with the differential expression of novel Brassica-specific transcripts. BMC Genomics, 2008, 9, 619.

  • Jako, C. et al., Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiol. Biochem., 2001, 126, 861–887.

  • Wen, O.-Y., Jianxiong, M. and Jingkai, D., Analytic study on the evaporable substances and fatty acids in aromatic (oriental) tobacco seeds. Acta Tabacaria Sin., 1996, 2, 51–55.

  • Slocombe, S. P. et al., Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways. Plant Biotechnol. J., 2009, 7, 694–703.

  • Gao, C.-Y., Mao, X., Shang, H.-Q., Ji, X.-J. and Li, R.-Z., Ectopic overexpression of MucACP-Δ9 desaturase leads to ω-7 fatty acid accumulation in tobacco leaves. Emir. J. Food Agric., 2016, 12, 890–896.


Abstract Views: 446

PDF Views: 129




  • Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels

Abstract Views: 446  |  PDF Views: 129

Authors

Chang-Yong Gao
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Xue Mao
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Hong-Qin Shang
Department of Life Science, Heze University, Heze, Shandong 274015, China
Fang Li
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China
Run-Zhi Li
Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, China

Abstract


To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5–5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.

Keywords


Biofuels, Metabolic Engineering, Plant Oil, Tobacco Leaves, VgDGAT1a Gene.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi06%2F1234-1240