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Isolation and Selection of Cellulose-Degrading Microorganisms for Utilization along with Earthworms in Efficient Conversion of Municipality Waste Mix to Compost


Affiliations
1 Microbial Resource Division, Institute of Bioresources and Sustainable Development, Takyelpat, Imphal 795 001, India
2 Department of Biotechnology, Gauhati University, Guwahati 781 014, India
3 Institute of Advanced Study in Science and Technology, Paschim Boragaon, Garchuk, Guwahati 781 035, India
 

Municipality biowaste (MBW) was decomposed alone or in mixture with rice straw (RS) and cow dung (CD) using cultures of Streptomyces xanthochomogens (CDM9) and Eisenia fetida. Cellulose degrading microorganisms (CDMs) were isolated from six source materials which contained CDM population in the range 5.4–8.5 log cfu/gdb and their cellulolytic activity ranged from 0.0 to 0.431 IU/min. CDM9 showed consistently high cellulase activity in cellulose and MBW substrate, and its co-inoculation with earthworm enhanced decomposition of the MBW mix. A positive correlation between pH of the composting feed mix and N loss suggested N loss in form of NH3. Overall, mixing of municipality biowaste with rice straw and cow dung and inoculation with the two decomposer agents resulted in better quality compost in all respects.

Keywords

Cellulose Degrading Microorganism, Municipality Biowaste, Nitrogen Loss, Pathogenic and Beneficial Bacteria.
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  • Isolation and Selection of Cellulose-Degrading Microorganisms for Utilization along with Earthworms in Efficient Conversion of Municipality Waste Mix to Compost

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Authors

Sanasam Shantibala Devi
Microbial Resource Division, Institute of Bioresources and Sustainable Development, Takyelpat, Imphal 795 001, India
Nongthombam Grihalakshmi Devi
Microbial Resource Division, Institute of Bioresources and Sustainable Development, Takyelpat, Imphal 795 001, India
Mohan Chandra Kalita
Department of Biotechnology, Gauhati University, Guwahati 781 014, India
Narayan Chandra Talukdar
Institute of Advanced Study in Science and Technology, Paschim Boragaon, Garchuk, Guwahati 781 035, India

Abstract


Municipality biowaste (MBW) was decomposed alone or in mixture with rice straw (RS) and cow dung (CD) using cultures of Streptomyces xanthochomogens (CDM9) and Eisenia fetida. Cellulose degrading microorganisms (CDMs) were isolated from six source materials which contained CDM population in the range 5.4–8.5 log cfu/gdb and their cellulolytic activity ranged from 0.0 to 0.431 IU/min. CDM9 showed consistently high cellulase activity in cellulose and MBW substrate, and its co-inoculation with earthworm enhanced decomposition of the MBW mix. A positive correlation between pH of the composting feed mix and N loss suggested N loss in form of NH3. Overall, mixing of municipality biowaste with rice straw and cow dung and inoculation with the two decomposer agents resulted in better quality compost in all respects.

Keywords


Cellulose Degrading Microorganism, Municipality Biowaste, Nitrogen Loss, Pathogenic and Beneficial Bacteria.

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DOI: https://doi.org/10.18520/cs%2Fv114%2Fi06%2F1261-1274