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Sperm Sexing of Dairy Cattle:Economics, Animal Welfare and Technological Challenges


Affiliations
1 Institute of Forensic Science and Criminology, Panjab University, Chandigarh 160 014, India
 

There is a growing need for sexed semen of cattle in India. Farmers prefer female calves of cows or buffalos than male calves as they can produce milk in the future which is economically more beneficial. Very few bulls are required for breeding in herds and they are no more required in agricultural fields due to mechanization of cultivation and transportation. Therefore these extra bulls/oxen are often sold at low prices to the meat industry. This is an animal welfare concern and can also cause communal tensions due to differences in belief systems of religious sects. At present there is no indigenous technology for producing sexed semen although artificial insemination with nonsexed semen is a well-established method of breeding among cattle farmers. The imported sexed semen is not easily available and also not affordable for most Indian farmers. There is a need for the development of cost effective indigenous sperm sexing techniques as an alternative to fluorescence-activated cell sorting. Recent developments in microfluidics and BEMS (bio electromechanical systems) may hold the key to the development of a portable device for semen sexing with minimal tampering of the sperm structure.

Keywords

Animal Welfare, Chromosomes, Sexed Semen of Cattle.
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  • http://dahd.nic.in/sites/default/files/19%20th%20Livestock%20%-202012.pdf
  • http://pib.nic.in/newsite/PrintRelease.aspx?relid=109280
  • Anshuman Kumar, M. R., Vineeth, Rebeka Sinha, Singh, R. K., Thakur, A. and Gupta, S. K., Current status, scope and constraints of sexed semen – an Indian perspective. Agric. Rev., 2016, 37(3), 240–244.
  • Vyawahare, M., Gender bias: India promotes sex selection in cattle to limit birth of male calves. Hindustan Times, 12 July 2017,
  • Sexed semen to increase female calf population. Times of India, 16 November 2014.
  • http://genusabsindia.com/imported-sexed-semen/
  • Kaur, S., American semen falls short on promise of delivering female calves. Indian Express, 11 April 2014.
  • Thomas, J. M., Locke, J. W. C., Vishwanath, R., Hall, J. B., Eller-sieck, M. R., Smith, M. F. and Patterson, D. J., Effective use of SexedULTRA™ sex-sorted semen for timed artificial insemination of beef heifers. Theriogenology, 2017, 98, 88–93.
  • Johnson, L. A. and Welch, G. R., Sex preselection: high-speed flow cytometric sorting of X and Y sperms for maximum efficiency. Theriogenology, 1999, 52(8), 1323–1341.
  • Jeyakumar, S., Kataktalware, M. A. and Ramesha, K. P. (eds), Invited lectures: Present Status and Future Prospects of Sexed Semen in India. National Seminar, Bengaluru, 24 January 2015.
  • McGrann, J., Gender value difference determines sexed semen advantage Progressive Cattleman, 30 September 2011; Meyer, T. L., Funston, R. N., Ranch, K. and McGrann, J. M., Sexing technologies, and ABS global, evaluating conventional and sexed semen in a commercial beef Heifer Program. Nebraska Beef Cattle Report, The Board of Regents of the University of Nebraska, 2012, pp. 20–21.
  • Raman, P., As Stray Cattle Wreak Havoc on Farmers, Why We Need a Policy for Ageing Cows, The Wire, Indian web publication, 8 September 2017.
  • Landes, M., Melton, A. and Edwards, S., From Where the Buffalo Roam: India’s Beef Exports, United States Department of Agriculture, 2016, pp. 1–6.
  • Buncombe, Andrew Nothing’s sacred: the illegal trade in India’s holy cows. The Independent, 1 June 2012.
  • Springmann, M., Godfray, H. C., Rayner, M. and Scarborough, P., Analysis and valuation of the health and climate change cobenefits of dietary change. Proc. Natl. Acad. Sci. USA, 2016, 113(15), 4146–4151.
  • Lu, Y., Zhang, M., Lu, S., Xu, D., Huang, W., Meng, B. and Lu, K., Sex-preselected buffalo (Bubalusbubalis) calves derived from artificial insemination with sexed sperm. Anim. Reprod. Sci., 2010, 119, 169–171.
  • Bhattacharya, B. C., Bangham, A. D., Cro, R. J., Keynes, R. D. and Rowson, L. E., An attempt to predetermine the sex of calves by artificial insemination with spermatozoa separated by sedimentation. Nature, 1966, 211(5051), 863.
  • Ali, J. I., Eldridge, F. E., Koo, G. C. and Schanbacher, B. D., Enrichment of bovine X- and Y-chromosome-bearing sperm with monoclonal H-Y antibody-fluorescence-activated cell sorter. Arch. Androl., 1990, 24(3), 235–245.
  • Hendriksen, P. J., Do X. and Y spermatozoa differ in proteins? Theriogenology, 1999, 52(8), 1295–1307.
  • Evans, K. M., Gilligan, T. B. and Gonzalez–Marin, C., Sperm staining and sorting methods. US Patent 9140688 B2, 2015; Cumming, I. R., Brewis, I. A. and Pitts, S. A., Identification of sex-linked proteins. Patent No. WO 2010150013 A2, 2010; Blecher, S. R. and Detmar, J., Sex-chromosome-specific proteins, species specific and sperm specific proteins and methods for their identification and isolation. Patent No. WO 2001042283 A2, 2001.
  • Engelmann, U., Krassnigg, F., Schatz, H. and Schill, W. B., Separation of human X and Y spermatozoa by free-flow electrophoresis. Gamete Res., 1988, 19(2), 151–160.
  • Promthep, K., Satitmanwiwat, S., Kitiyanant, N., Tantiwattanakul, P., Jirajaroenrat, K., Sitthigripong, R. and Singhapol, C., Practical use of percoll density gradient centrifugation on sperm sex determination in commercial dairy farm in Thailand. Indian J. Anim. Res., 2016, 50(3), 310–313.
  • Grant, V. J., Entrenched misinformation about X and Y sperm. Br. Med. J., 2006, 332(7546), 916.
  • Evans, K. M., Gilligan, T. B. and Gonzalez–Marin, C., Sperm staining and sorting methods. US Patent 9140688 B2, 2015.
  • Sharpe, P. T., Bovine semen sexing. US Patent No. WO 1993010803 A1, 1993.
  • Mileham, A., Affara, N., Plastow, G. and Pig Improvement Co (UK) Ltd, Methods for sexing non-human mammals. US Patent no. CA 2389906 A1, 10 May 2001.
  • Parati, K., Bongioni, G., Aleandri, R. and Galli, A., Sex ratio determination in bovine semen: a new approach by quantitative real time PCR. Theriogenology, 2006, 66(9), 2202–2209.
  • Alves, B. C., Hossepian de Lima, V. F., Teixeira, C. M. and Moreira–Filho, C. A., Use of primers derived from a new sequence of the bovine Y chromosome for sexing Bostaurus and Bosindicus embryos. Theriogenology, 2003, 59(5–6), 1415–1419.
  • Kageyama, S., Yoshida, I., Kawakura, K. and Chikuni, K., A novel repeated sequence located on the bovine Y chromosome: its application to rapid and precise embryo sexing by PCR. J. Vet. Med. Sci., 2004, 66(5), 509–514.
  • Chen, M. J., Guu, H. F. and Ho, E. S., Efficiency of sex preselection of spermatozoa by albumin separation method evaluated by double-labelled fluorescence in-situ hybridization. Hum. Reprod., 1997, 12(9), 1920–1926; Beernink, F. J., Dmowski, W. P. and Ericsson, R. J., Sex pre-selection through albumin separation of sperm. Fertil. Steril., 1993, 59(2), 382–386.
  • Seidel Jr, G. E., Schenk, J. L., Herickhoff, L. A., Doyle, S. P., Brink, Z., Green, R. D. and Cran, D. G., Insemination of heifers with sexed sperm. Theriogenology, 1999, 52(8), 1407–1420.
  • Parrilla, I., Vazquez, J. M. and Cuello, C., Hoechst 33342 stain and UV laser exposure do not induce genotoxic effects in flowsorted boar spermatozoa. Reproduction, 2004, 128, 615–621.
  • Garner, D. L., Flow cytometric sexing of mammalian sperm. Theriogenology, 2006, 65, 943–957.
  • Carvalho, J. O., Sartori, R., Machado, G. M., Mourão, G. B. and Dode, M. A. N., Quality assessment of bovine cryopreserved sperm after sexing by flow cytometry and their use in in vitro embryo production. Theriogenology, 2010, 74, 1521–1530.
  • Bilodeau, J. F., Blanchette, S., Cormier, N. and Sirard, M. A., Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by Pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology, 2002, 57(3), 1105–1122.
  • Shiono, H. and Ito, Y., Novel method for continuous cell separation by density gradient centrifugation: evaluation of a miniature separation column. Prep. Biochem. Biotechnol., 2003, 33(2), 87–100.
  • Fu, A. Y., Spence, C., Scherer, A., Arnold, F. H. and Quake, S. R., A microfabricated fluorescence-activated cell sorter. Nat. Biotechnol., 1999, 17(11), 1109–1111; Knowlton, S. M., Sadasivam, M. and Tasoglu, S., Microfluidics for sperm research. Trends Biotechnol., 2015, 33(4), 221–229.
  • De Wagenaar, B., Berendsen, J. T., Bomer, J. G., Olthuis, W., van den Berg, A. and Segerink, L. I., Microfluidic single sperm entrapment and analysis. Lab Chip, 2015, 15(5), 1294–1301.
  • Sarkar, S., Jolly, D. J., Friedmann, T. and Jones, O. W., Swimming behaviour of X and Y human sperm. Differentiation, 1984, 27(2), 120–125.
  • Huang, H.-Y., Fu, H.-T., Tsing, H.-Y., Huang, H.-J., Li, C.-J. and Yao, D.-J., Motile human sperm sorting by an integrated microfluidic system. J. Nanomed. Nanotechnol., 2014, 5, 199.
  • Segerink, L. I., Sprenkels, A. J., Oosterhuis, G. J. E., Vermes, I. and van den Berg, A., Microfluidic chips for semen analysis. EJIFCC, 2012, 23(3), 66–69.
  • Lai, D., Smith, G. D. and Takayama, S., Lab-on-a-chip biophotonics: its application to assisted reproductive technologies. J. Biophotonics, 2012, 5(8–9), 650–660.
  • Chung, Y., Zhu, X., Gu, W., Smith, G. D. and Takayama, S., Microscale integrated sperm sorter. Methods Mol. Biol., 2006, 321, 227–244.
  • Mori, Y., Size-selective separation techniques for nanoparticles in liquid. KONA Powder Particle J., 2015, 32, 102–114.
  • Bae, J., Kim, W., Rah, K., Jung, E. C. and Lee, S., Application of flow field-flow fractionation (FlFFF) for size characterization of carbon black particles in ink. Microchem. J., 2012, 104, 44–48.
  • Baalousha, M., Kammer, F. V. D., Motelica-Heino, M. and Le Coustumer, P., Natural sample fractionation by FlFFF–MALLS–TEM: Sample stabilization, preparation, preconcentration and fractionation. J. Chromatogr. A, 2005, 1093(1–2), 156–166.

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  • Sperm Sexing of Dairy Cattle:Economics, Animal Welfare and Technological Challenges

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Authors

Jagdish Rai
Institute of Forensic Science and Criminology, Panjab University, Chandigarh 160 014, India

Abstract


There is a growing need for sexed semen of cattle in India. Farmers prefer female calves of cows or buffalos than male calves as they can produce milk in the future which is economically more beneficial. Very few bulls are required for breeding in herds and they are no more required in agricultural fields due to mechanization of cultivation and transportation. Therefore these extra bulls/oxen are often sold at low prices to the meat industry. This is an animal welfare concern and can also cause communal tensions due to differences in belief systems of religious sects. At present there is no indigenous technology for producing sexed semen although artificial insemination with nonsexed semen is a well-established method of breeding among cattle farmers. The imported sexed semen is not easily available and also not affordable for most Indian farmers. There is a need for the development of cost effective indigenous sperm sexing techniques as an alternative to fluorescence-activated cell sorting. Recent developments in microfluidics and BEMS (bio electromechanical systems) may hold the key to the development of a portable device for semen sexing with minimal tampering of the sperm structure.

Keywords


Animal Welfare, Chromosomes, Sexed Semen of Cattle.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi07%2F1438-1442