Current Science

Open Access Open Access  Restricted Access Subscription Access

RNA Base Editing:Programmable Protein Machine


Affiliations
1 Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
 

RNA (ribonucleic acid) is a unique molecule which plays important and diverse roles in biology. In cells, RNA is ephemeral; it acts as a mediator, carrying genetic information from the nucleus to the protein-making machinery in the cytoplasm. A point mutation in RNA transcript could make the protein much shorter or larger than normal. Aberrant expression of RNAs effects the regulation of many cellular functions and gene networks. Most human genetic disorders such as sickle cell anaemia are due to just a single base-pair substitution, or by errors in RNA splicing, or its regulation. Precise editing of aberrant RNA provides a platform to correct the errors, without altering the genetic make-up of template DNA.
User
Notifications
Font Size

  • Cooper, T. A., Wan, L. and Dreyfuss, G., Cell, 2009, 136, 777–793.

  • Douglas, A. G. L. and Wood, M. J. A., Brief. Funct. Genomics, 2011, 10, 151–164.

  • Doudna, J. A. and Charpentier, E., Science, 2014, 346, 1258096.

  • Dominguez, A. A., Lim, W. A. and Qi, L. S., Nature Rev. Mol. Cell Biol., 2016, 17, 5–15.

  • Shmakov, S., Abudayyeh, O. O. and Makarova, K. S., Mol. Cell, 2015, 60, 383–397.

  • Abudayyeh, O. O. et al., Science, 2016, 353, aaf5573.

  • East-Seletsky, A., O’Connell, M. R. and Knight, S. C., Nature, 2016, 538, 270–273.

  • Abudayyeh, O. O. et al., Nature, 2017, 550, 280.

  • Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellener, M. J., Joung, J. and Zhang, F., Science, 2017, 358, 1019–1027.

  • Nishikura, K., Nature Rev. Mol. Cell Biol., 2016, 17, 83–96.

  • Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A. and Liu, D. R., Nature, 2016, 533, 420–436.

  • Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I. and Liu, D. R., Nature, 2017, 551, 464–471.

  • Yang, L. and Chen, L. L., Science, 2017, 358, 996–997.


Abstract Views: 636

PDF Views: 122




  • RNA Base Editing:Programmable Protein Machine

Abstract Views: 636  |  PDF Views: 122

Authors

Kulbhushan Chaudhary
Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India

Abstract


RNA (ribonucleic acid) is a unique molecule which plays important and diverse roles in biology. In cells, RNA is ephemeral; it acts as a mediator, carrying genetic information from the nucleus to the protein-making machinery in the cytoplasm. A point mutation in RNA transcript could make the protein much shorter or larger than normal. Aberrant expression of RNAs effects the regulation of many cellular functions and gene networks. Most human genetic disorders such as sickle cell anaemia are due to just a single base-pair substitution, or by errors in RNA splicing, or its regulation. Precise editing of aberrant RNA provides a platform to correct the errors, without altering the genetic make-up of template DNA.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi09%2F1808-1809