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Mechanical Properties and Differentiation Assessments of Neural Stem Cells with Pneumatic Micropipette Aspiration


Affiliations
1 State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Qufu Normal University, Qufu 273165, China
2 Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China
3 TEDA Hospital, No. 65 Third Avenue, Economic-Technological Development Area, Tianjin 300457, China
4 State Key Laboratory of Medicinal Chemical Biology, College of Life Science, College of Life Sciences, Qufu Normal University, Qufu 273165, China
 

The change in chemical and biological properties of neural stem cells (NSCs) before and after differentiating into neurons and glial cells has been well studied. However, there is lack of knowledge on the relationship between cell differentiation and alteration of cell mechanical features. Mechanical properties can reflect specific changes that occur with biochemical and cytological changes. Here, we present a robotic micromanipulation system for measuring the mechanical properties of single cells. This system consists of a suction micropipette, a robotic micromanipulator and an inverted microscope. A pneumatic micropipette aspiration method is utilized to measure the elastic properties of the cells. We found that the mechanical properties of NSCs belong to the solid state, however, neurons and glial cells are close to the liquid state. Further, NSCs are harder than neurons and glial cells.

Keywords

Mechanical Properties, Robotic Micromanipulation System, Differentiation Assessment, Neural Stem Cells.
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  • Mechanical Properties and Differentiation Assessments of Neural Stem Cells with Pneumatic Micropipette Aspiration

Abstract Views: 337  |  PDF Views: 122

Authors

Ming Wu
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Qufu Normal University, Qufu 273165, China
Qili Zhao
Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China
Cui Yang
TEDA Hospital, No. 65 Third Avenue, Economic-Technological Development Area, Tianjin 300457, China
Wen Shen
TEDA Hospital, No. 65 Third Avenue, Economic-Technological Development Area, Tianjin 300457, China
Xin Zhao
Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China
Yun Lu
TEDA Hospital, No. 65 Third Avenue, Economic-Technological Development Area, Tianjin 300457, China
Xi Zeng Feng
State Key Laboratory of Medicinal Chemical Biology, College of Life Science, College of Life Sciences, Qufu Normal University, Qufu 273165, China

Abstract


The change in chemical and biological properties of neural stem cells (NSCs) before and after differentiating into neurons and glial cells has been well studied. However, there is lack of knowledge on the relationship between cell differentiation and alteration of cell mechanical features. Mechanical properties can reflect specific changes that occur with biochemical and cytological changes. Here, we present a robotic micromanipulation system for measuring the mechanical properties of single cells. This system consists of a suction micropipette, a robotic micromanipulator and an inverted microscope. A pneumatic micropipette aspiration method is utilized to measure the elastic properties of the cells. We found that the mechanical properties of NSCs belong to the solid state, however, neurons and glial cells are close to the liquid state. Further, NSCs are harder than neurons and glial cells.

Keywords


Mechanical Properties, Robotic Micromanipulation System, Differentiation Assessment, Neural Stem Cells.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi09%2F1961-1966