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Accounting for Secondary Inner Filter Effect in Fluorescence Spectra from Solid Samples


Affiliations
1 Department of Applied Chemistry, Shri Govindram Seksaria Institute of Technology and Science, Indore 452 003, India
2 School of Chemical Sciences, National Institute of Science Education and Research Bhubaneswar, Jatni 752 050, India
3 Department of Chemistry, National Institute of Technology Calicut, Kozhikode 673 601, India
4 Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, India
 

Fluorescence experiments at high fluorophore concentrations or in solid state have biological relevance and application potential in devices. Inner filter effect, arising from reabsorption of emitted photons, is the simplest of several factors that complicate the emission in these systems. This effect is prominent, especially for systems with small Stokes shift between absorption and emission. Solid state ‘dilution’ of a fluorophore with an optically transparent, non-interfering substance can significantly minimize this effect. This has been shown in the present study, using salophen as a fluorophore and NaCl as the dilutant. The general applicability of this method is tested with three more fluorophores.

Keywords

Aggregation-Induced Emission, Inner Filter Effect, Fluorescence Spectroscopy, Solid-State Emitters.
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  • Accounting for Secondary Inner Filter Effect in Fluorescence Spectra from Solid Samples

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Authors

Tuhin Khan
Department of Applied Chemistry, Shri Govindram Seksaria Institute of Technology and Science, Indore 452 003, India
Kalyani Bhagabani
School of Chemical Sciences, National Institute of Science Education and Research Bhubaneswar, Jatni 752 050, India
Preeti Kumari
Department of Chemistry, National Institute of Technology Calicut, Kozhikode 673 601, India
Anindya Datta
Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, India

Abstract


Fluorescence experiments at high fluorophore concentrations or in solid state have biological relevance and application potential in devices. Inner filter effect, arising from reabsorption of emitted photons, is the simplest of several factors that complicate the emission in these systems. This effect is prominent, especially for systems with small Stokes shift between absorption and emission. Solid state ‘dilution’ of a fluorophore with an optically transparent, non-interfering substance can significantly minimize this effect. This has been shown in the present study, using salophen as a fluorophore and NaCl as the dilutant. The general applicability of this method is tested with three more fluorophores.

Keywords


Aggregation-Induced Emission, Inner Filter Effect, Fluorescence Spectroscopy, Solid-State Emitters.

References





DOI: https://doi.org/10.18520/cs%2Fv114%2Fi11%2F2353-2356