Obtaining 3D and 3D Revert Cultures of BMG-1 Cell Line for the Analysis of Cytokine Expression Differences

Methods to utilize cell lines as research material are evolving continuously as with the parallel advancement in instrumentation and analysis technologies. One such advancement in culture methodology of particular significance is the 3-dimensional (3D) way of culturing cells. It is now clear that 3D cultured cells behave differently from their monolayer (2D) counterparts and provide meaningful insights into complex cellular mechanisms that are rather difficult to study using 2D cultured cells. We take a step further and describe 3D-reverts, an extension of the ‘2D to 3D’ culture methodology. We demonstrate that 2D, 3D and 3DRs express cytokines differently and also that such differences extend to the culture stages of 3D and 3DRs, in a time-dependent manner. This approach of analysing differences between 3D and 3DRs as a timedependent or culture stage-dependent manner will surely enhance the utility of cells that will augment the 3D culture systems.