Current Science

Open Access Open Access  Restricted Access Subscription Access

Preparation and Characterization of PLGA-Based Biocompatible Nanoparticles for Sustained Delivery of Growth Factor for Wound Healing Applications


Affiliations
1 Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
 

Platelet-derived growth factor-BB (PDGF-BB) loaded PLGA : poloxamer blend nanoparticles (PLGF) were studied for their physical, chemical and biological characteristics using in vitro and in vivo experimental models. The particle size of PLGF was between 100 and 150 nm with 85% encapsulation efficiency. In vitro released kinetics revealed a slow and sustained release of growth factor (GF) for a month. Furthermore, biological studies confirmed the non-toxic, and non-irritant nature of nanoparticles and angiogenesis stimulating ability of GF released from nanoparticles demonstrating their potential for the treatment of chronic wounds in future.

Keywords

PDGF-BB, PLGA, Angiogenesis, Wound Healing.
User
Notifications
Font Size

  • Mulder, G. D. and Vande Berg, J. S., Cellular senescence and matrix metalloproteinase activity in chronic wounds. Relevance to debridement and new technologies. J. Am. Pediatr. Med. Assoc., 2002, 92, 34-37.

  • Mustoe, T., Understanding chronic wounds: a unifying hypothesis on their pathogenesis and implications for therapy. Am. J. Surg., 2004, 187, 65S-70S.

  • Vande Berg, J. S. and Robson, M. C., Arresting cell cycles and the effect on wound healing. Surg. Clin. N. Am., 2003, 83, 509-520.

  • Rayner, R., Carville, K. J., Prentice, K. J. and Santamaria, X. N., Leg ulcers: atypical presentations and associated comorbidities. WoundPract. Res., 2009, 17(4), 168-185.

  • Agren, M. S., Proliferation and mitogenic response to PDGF-BB of fibroblasts isolated from chronic venous leg ulcers is ulcer-age dependent. J. Invest. Dermatol., 1999, 112, 463-469.

  • Pierce, G. F. and Mustoe, T. A., Pharmacologic enhancement of wound healing. Annu. Rev. Med., 1995, 46, 467-481.

  • Obara, K., Ishihara, M., Ishizuka, T., Fujita, M., Ozeki, Y. and Maehara, T., Photo crosslinkable chitosan hydrogel containing fibroblast growth factor-2 stimulates wound healing in healingimpaired db/db mice. Biomaterials, 2003, 24, 3437-3444.

  • Cooke, J. W., Sarment, D. P., Whitesman, L. A., Miller, S. E., Jin, Q., Lynch, S. E and Giannobile, W. V., Effect of rhPDGF-BB delivery on mediators of periodontal wound repair. Tissue Eng., 2006, 12(6), 1441-1450.

  • Schultz, G. S., Sibbald, R. G., Falanga, V., Ayello, E. A., Dowsett, C. and Harding, K., Wound bed preparation: a systematic approach to wound management. Wound Repair R egen., 2003, 11, S1-S28.

  • Jain, R., Agarwal, A., Kierski, P. R., Schurr, M. J., Murphy, C. J. and McAnulty, J. F., The use of native chemical functional groups presented by wound beds for the covalent attachment of polymeric microcarriers of bioactive factors. Biomaterials, 2013, 34, 340-352.

  • Csaba, N., Caamano, P., Sanchez, A., Dominguez, F. and Alonso, M. J., PLGA: poloxamer and PLGA: poloxamine blend NPs: new carriers for gene delivery. Biomacromolecules, 2005, 6(1), 271-278.

  • Mukerjee, A. and Vishwanatha, J. K., Formulation, characterization and evaluation of curcumin-loaded PLGA nanospheres for cancer therapy. Anticancer Res., 2009, 29(10), 3867-3875.

  • d’Angelo, I. et al. NPs based on PLGA: poloxamer blends for the delivery of proangiogenic growth factors. Mol. Pharm., 2010, 7(5), 1724-1733.

  • Liang, C. C., Park, A. Y. and Guan, J. L., In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat. Protoc., 2007, 2(2), 329-333.

  • Auerbach, R., Akhtar, N. Lewis, R. L. and Shinners, B. L., Angiogenesis assays: problems and pitfalls. CancerMetast. Rev., 2000, 19(1 and 2), 167-172.

  • Morton, J. J. and Malone, M. H., Evaluation of vulnerary activity by an open wound procedure in rats. Arch. Int. Pharmacodyn. Therap., 1972, 196(1), 117-126.

  • Baie, S. H. and Sheikh, K. A., The wound healing properties of Channa striatuscetrimide cream-tensile strength measurement. J. Ethnopharmacol., 2000, 71, 93-110.

  • Kabanov, V. A. and Alakhov, V., Pluronic block copolymers in drug delivery: from micellar nanocontainers to biological response modifiers. Crit. Rev. Ther. Drug Carr. Syst., 2002, 19(1), 1-72.

  • Goodwin, C. J. Holt, S. J., Downes, S. and Marshall, N. J., Microculture tetrazolium assays: a comparison between two new tetrazolium salts, XTT and MTS. J. Immunol. Meth., 1995, 179(1), 95-103.

  • Batycky, R. P. Hanes, J., Langer, R. and Edwards, D. A., A theoretical model of erosion and macromolecular drug release from biodegrading microspheres. J. Pharm. Sci., 1997, 86(12), 1464-1477.

  • Gallagher, K. M. and Corrigan, O. I., Mechanistic aspects of the release of levamisole hydrochloride from biodegradable polymers. J. Control. Release, 2000, 69(2), 261-272.

  • Santander-Ortega, M. J., Csaba, N., Gonzalez, L., BastosGonzalez, D., Ortega-Vinuesa, J. and Alonso, M. J., Protein loaded PLGE-PEOblend nanoparticles: encapsulation, release and degradation characteristics. Colloid Polym. Sci., 2010, 288, 141-150.

  • Pierce, G. F. et al., Tissue repair processes in healing chronic pressure ulcers treated with recombinant platelet-derived growth factor BB. Am. J. Pathol, 1994, 145, 1399-1410.


Abstract Views: 329

PDF Views: 110




  • Preparation and Characterization of PLGA-Based Biocompatible Nanoparticles for Sustained Delivery of Growth Factor for Wound Healing Applications

Abstract Views: 329  |  PDF Views: 110

Authors

E. Santhini
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
V. M. Pramila
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
M. Shalini
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
R. Vignesh Balaji
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
K. P. Chellamani
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India

Abstract


Platelet-derived growth factor-BB (PDGF-BB) loaded PLGA : poloxamer blend nanoparticles (PLGF) were studied for their physical, chemical and biological characteristics using in vitro and in vivo experimental models. The particle size of PLGF was between 100 and 150 nm with 85% encapsulation efficiency. In vitro released kinetics revealed a slow and sustained release of growth factor (GF) for a month. Furthermore, biological studies confirmed the non-toxic, and non-irritant nature of nanoparticles and angiogenesis stimulating ability of GF released from nanoparticles demonstrating their potential for the treatment of chronic wounds in future.

Keywords


PDGF-BB, PLGA, Angiogenesis, Wound Healing.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi7%2F1287-1296