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Preparation and Characterization of PLGA-Based Biocompatible Nanoparticles for Sustained Delivery of Growth Factor for Wound Healing Applications


Affiliations
1 Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
 

Platelet-derived growth factor-BB (PDGF-BB) loaded PLGA : poloxamer blend nanoparticles (PLGF) were studied for their physical, chemical and biological characteristics using in vitro and in vivo experimental models. The particle size of PLGF was between 100 and 150 nm with 85% encapsulation efficiency. In vitro released kinetics revealed a slow and sustained release of growth factor (GF) for a month. Furthermore, biological studies confirmed the non-toxic, and non-irritant nature of nanoparticles and angiogenesis stimulating ability of GF released from nanoparticles demonstrating their potential for the treatment of chronic wounds in future.

Keywords

PDGF-BB, PLGA, Angiogenesis, Wound Healing.
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  • Preparation and Characterization of PLGA-Based Biocompatible Nanoparticles for Sustained Delivery of Growth Factor for Wound Healing Applications

Abstract Views: 313  |  PDF Views: 101

Authors

E. Santhini
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
V. M. Pramila
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
M. Shalini
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
R. Vignesh Balaji
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India
K. P. Chellamani
Centre of Excellence for Medical Textiles, The South India Textile Research Association (SITRA), Coimbatore 641 014, India

Abstract


Platelet-derived growth factor-BB (PDGF-BB) loaded PLGA : poloxamer blend nanoparticles (PLGF) were studied for their physical, chemical and biological characteristics using in vitro and in vivo experimental models. The particle size of PLGF was between 100 and 150 nm with 85% encapsulation efficiency. In vitro released kinetics revealed a slow and sustained release of growth factor (GF) for a month. Furthermore, biological studies confirmed the non-toxic, and non-irritant nature of nanoparticles and angiogenesis stimulating ability of GF released from nanoparticles demonstrating their potential for the treatment of chronic wounds in future.

Keywords


PDGF-BB, PLGA, Angiogenesis, Wound Healing.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi7%2F1287-1296