Current Science

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


In this study, flavonoid-rich chloroform fraction (FRCF) of nine different citrus species was isolated by solvent fractionation technique and its effect on α- glucosidase, α-amylase, glucose uptake by yeast cells and glycosylation of haemoglobin was studied. FRCF of Citrus maxima was found to show higher activity in the in vitro assays and thus it was characterized by Electrospray Ionization Mass Spectrometry (ESI-MS) analysis. This led to the identification of icariin, hesperidin and diosmetin-6,8-di-C-glucoside. Their binding efficacy with targeted proteins such as glucokinase (GK), glycogen synthase kinases 3β (GSK 3β) and peroxisome proliferator-activated receptor-γ (PPAR-γ ) was studied by in silico analysis using Schrödinger Maestro software. Icariin exhibited maximum docking energy with GSK 3β, relatively less for GK and null for PPAR-γ. Hesperidin and diosmetin-6, 8-di-C-glucoside showed good binding affinity with PPAR-γ compared to GSK 3β.

Keywords

Anti-Hyperglycaemic Activity, Bioflavonoids, Citrus maxima, Yeast Cells.
User
Notifications
Font Size