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Comparative Expression Analysis of Defence-Related Genes in Bacillus-Treated Glycine Max upon Challenge Inoculation with Selective Fungal Phytopathogens


Affiliations
1 Mandsaur University, Mandsaur 458 001, India
2 Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India
 

Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT– PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethyl-hexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively.

Keywords

Defence-Related Genes, Induced Systemic Resistance, Plant Growth Promoting Bacteria, Volatile Organic Compounds.
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  • Comparative Expression Analysis of Defence-Related Genes in Bacillus-Treated Glycine Max upon Challenge Inoculation with Selective Fungal Phytopathogens

Abstract Views: 357  |  PDF Views: 111

Authors

Shekhar Jain
Mandsaur University, Mandsaur 458 001, India
Anukool Vaishnav
Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India
Ajit Varma
Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India
Devendra Kumar Choudhary
Amity Institute of Microbial Technology, Amity University, Noida 201 303,, India

Abstract


Activation of defence-related genes by the application of beneficial bacteria leads to prior protection against pathogens through induced systemic resistance. The present study was carried out to examine the qRT– PCR-based relative quantification of differently expressed defence-related genes in soybean (Glycine max L. Merrill) plants primed with Bacillus sp. strain SJ-5 against the fungal pathogen Rhizoctonia solani and Fusarium oxysporum. In this context, molecular characterization of plant growth promoting and biocontrol genes of SJ-5 was done by PCR followed by homology analysis. In the GC-MS analysis of SJ-5 volatile organic compounds, potent antifungal compound bis(2-ethyl-hexyl) phthalate and antioxidant compound butylated hydroxy toluene were reported with the highest peak area 47.96% and 21.82% respectively, along with other antifungal compounds in small proportion. Qualitative expression of different defence-related genes like lipoxygenase, phenylalanine ammonia-lyase 2, peroxidase, polyphenol oxidase, endo-1,3-beta-glucanase, catalase, defensin-like protein, vegetative storage protein and chitinase class I was found elicited in the plants primed with SJ-5 against the fungal pathogens. In the qPCR analysis, the highest upregulation was observed in the transcript profile of ppojh2 in the treatments T5 and T6 with 4.12- and 4.06-fold increase respectively.

Keywords


Defence-Related Genes, Induced Systemic Resistance, Plant Growth Promoting Bacteria, Volatile Organic Compounds.

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DOI: https://doi.org/10.18520/cs%2Fv115%2Fi10%2F1950-1956