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Artificial Seed for Short-Term Storage:Using Nodal Buds in Aquilaria malaccensis Lam


Affiliations
1 Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
 

Artificial seed technology is known to be the best alternative for short-term storage of plant germplasm in recalcitrant species. Aquilaria malaccensis, being a recalcitrant seeded tree, possesses seasonal and highly desiccation-sensitive seeds which lose viability with slight decrease in their moisture content, hence storage becomes a difficult task. The present study deals with short-term storage of A. malaccensis nodal buds through artificial seed technology. Nodal buds encapsulated with 2.5% sodium alginate and 100 mM calcium chloride, and polymerized for 40 min were found suitable for artificial seed preparation. Maximum regeneration rate of 83.3% and 75.0% was observed from encapsulated nodal buds stored at 4°C and 23 ± 2°C respectively, for 10 days. Storage was possible for a period of 60 days at 4°C and 50 days at 23 ± 2°C with average regeneration rate of 8.3% and 16.7% respectively.

Keywords

Artificial Seeds, Encapsulation, Recalcitrant, Storage, Temperature.
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  • Filho, A. R., Dal Vesco, L. L., Nodari, R. O., Lischka, R. W., Müller, C. V. and Guerra, M. P, Tissue culture for the conservation and mass propagation of Vriesea reitzii Leme and Costa, a bromeliad threatened of extinction from the Brazilian Atlantic Forest. Biodivers. Conserv., 2005, 14, 1799–1808.

  • Bewley, J. D. and Black, M., Seeds: Physiology of Development and Germination, Plenum Press, New York, USA, 1985, p. 367.

  • Harding, K., Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 2004, 25, 3–22.

  • Micheli, M., Hafiz, I. A. and Standardi, A., Encapsulation of in vitro-derived plantlets of olive (Olea europaea L. cv. Moraiolo) II. Effects of storage on capsule and derived shoots performance. Sci. Hortic., 2007, 113, 286–292.

  • Rai, M. K., Jaiswal, V. S. and Jaiswal, U., Alginate-encapsulation of nodal segments of guava (Psidium guajava L.) for germplasm exchange and distribution. J. Hortic. Sci. Biotechnol., 2008, 83, 569–573.

  • Rai, M. K., Jaisal, V. S. and Jaiswal, U., Encapsulation of shoot tips of guava (Psidium guajava L.) for short-term storage and germplasm exchange. Sci. Hortic., 2008, 118, 33–38.

  • Naik, S. K. and Chand, P. K., Nutrient–alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci. Hortic., 2006, 108, 247–52.

  • Mandal, J., Pattnaik, S. and Chand, P. K., Alginate encapsulation of axillary buds of Ocimum americanum L. (hoary basil), O. basilicum L. (sweet basil), O. gratissimum L. (shrubby basil), and O. sanctum (sacred basil). In vitro Cell. Dev. Biol. – Plant, 2000, 36, 287–292.

  • Nandini, B. P., Sudarshana, M. S. and Rajashekar, N., Plant regeneration through somatic embryogenesis and synthetic seed production in Rumex vesicarius L. – a potent medicinal herb. IOSR J. Pharm. Biol. Sci., 2014, 9, 129–136.

  • Saikia, P. and Khan, M. L., Aquilaria malaccensis Lam., a Redlisted and highly exploited tree species in the Assamese home garden. Curr. Sci., 2012, 102, 546–547.

  • Shrivastava, K., Anuradha, K. and Tasso, T., The role of fungi in the production of aromatic agarwood in Aquilaria agallocha (Roxb.). A commercially important medicinal tree species of Arunachal Pradesh. In Forest Biotechnology in India (eds Ansari, S. A., Narayanan, C. and Mandal, A. K.), Satish Serial Publishing House, Delhi, 2008, pp. 275–283.

  • Shankar, U., Effect of seed abortion and seed storage on germination and seedling growth in Aquilaria malaccensis Lamk. (Thymelaeaceae). Curr. Sci., 2012, 102, 596–604.

  • Venugopal, N. and Marbaniang, E. J., Observation on the presence of an aril in the seeds of Aquilaria malaccensis Lam. (syn. A. agallocha Roxb.) (Thymelaeaceae) growing in Meghalaya, North-east India. Int. J. Plant Reprod. Biol., 2015, 7, 189–194.

  • Tabin, T., A thesis on studies on fungal diversity associated with Aquilaria agallocha Roxb. and their role in the formation of agarwood, Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli, 2012.

  • Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures. J. Plant Physiol., 1962, 15, 473–497.

  • Singh, A. K., Sharma, M., Varshney, R., Agarwal, S. S. and Bansal, K. C., Plant regeneration from alginate encapsulated shoot tips of Phlyllanthus amarus Schum and Thonn, medicinally important plant species. In vitro Cell. Dev. Biol. – Plant, 2006, 42, 109–113.

  • Rai, M. K., Asthana, P., Singh, S. K., Jaisal, V. S. and Jaiswal, U., The encapsulation technology in fruits plants – a review. Biotechnol. Adv., 2009, 27, 671–679.

  • Maqsood, M., Mujib, A. and Siddiqui, H., Synthetic seed development and conversion to plantlet in Catharanthus roseus (L.) G. Don. Biotechnology, 2012, 11, 37–43.

  • West, T. P., Ravindra, M. B. and Precce, J. E., Encapsulation, cold storage, and growth of Hibiscus moscheutos nodal segments. Plant Cell Tissue Organ Cult., 2006, 87, 223–231.

  • Chand, S. and Singh, A. K., Plant regeneration from encapsulated nodal segments of Dalbergia sissoo Roxb., a timber-yielding leguminous tree species. J. Plant Physiol., 2004, 161, 237– 243.

  • Thiruvengadam, M., Praveen, N. and Chung, I., Plant regeneration from alginate-encapsulated shoot tips of Momordica dioica for short term storage and germplasm exchange and distribution. Plant Omics J., 2012, 5, 266–270.

  • Duad, N., Taha, R. M. and Hasbullah, N. A., Artificial seed production from encapsulated micro shoots of Saintpaulia ionantha Wendl. (African violet). J. Appl. Sci., 2008, 8, 4662–4667.

  • Islam, M. S. and Bari, M. A., In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L. J. Biol. Sci., 2012, 20, 99–108.

  • Sakhanokho, H. F., Pounders, C. T. and Blythe, E. K., Alginate encapsulation of Begonia microshoots for short-term storage and distribution. Sci. World J., 2013, 13, 1–7.

  • Das, M. C., Kumaria, S. and Tandon, P., In vitro propagation and conservation of Dendrobium lituiflorum Lindl. through protocormlike bodies. J. Plant Biochem. Biotechnol., 2008, 17, 177–180.

  • Redenbaugh, K., Fujii, J. A., Slade, D., Viss, P. R. and Kossler, M. E., Artificial seeds-encapsulated embryos. In Biotechnology in Agriculture and Forestry, High Technology and Micropropagation (ed. Bajai, Y. P. S.), Springer-Verlag. Heidelberg, Germany, 1991, pp, 395–416.

  • Mohanty, P., Nongkling, P., Das, M. C., Kumaria, S. and Tandon, P., Short-term storage of alginate-encapsulated protocorm-like bodie of Dendrobium nobile Lindl.: an endangered medicinal orchid from North-east India. Biotech, 2013, 3, 235–239.

  • Redenbaugh, K., Slade, D., Viss, P. and Fujii, J. A., Encapsulation of somatic embryos in synthetic seed coats. Hortic. Sci., 1987, 22, 803–809.


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  • Artificial Seed for Short-Term Storage:Using Nodal Buds in Aquilaria malaccensis Lam

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Authors

Seram Devika Devi
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
Bateimon Kharsahnoh
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
Suman Kumaria
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India
Meera C. Das
Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793 022, India

Abstract


Artificial seed technology is known to be the best alternative for short-term storage of plant germplasm in recalcitrant species. Aquilaria malaccensis, being a recalcitrant seeded tree, possesses seasonal and highly desiccation-sensitive seeds which lose viability with slight decrease in their moisture content, hence storage becomes a difficult task. The present study deals with short-term storage of A. malaccensis nodal buds through artificial seed technology. Nodal buds encapsulated with 2.5% sodium alginate and 100 mM calcium chloride, and polymerized for 40 min were found suitable for artificial seed preparation. Maximum regeneration rate of 83.3% and 75.0% was observed from encapsulated nodal buds stored at 4°C and 23 ± 2°C respectively, for 10 days. Storage was possible for a period of 60 days at 4°C and 50 days at 23 ± 2°C with average regeneration rate of 8.3% and 16.7% respectively.

Keywords


Artificial Seeds, Encapsulation, Recalcitrant, Storage, Temperature.

References





DOI: https://doi.org/10.18520/cs%2Fv115%2Fi11%2F2103-2109