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Production of Novel Bio-Flocculants from Klebsiella variicola BF1 using Cassava Starch Wastewater and its Application


Affiliations
1 Toxicology and Biomedicine Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam
2 Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, Province of China
3 Ton Duc Thang University, Ho Chi Minh City, Viet Nam
 

In this study, Klebsiella variicola BF1 that uses cassa-va starch wastewater to produce flocculants was identified using 16S rDNA gene sequencing. The pure flocculants of strain BF1 could be easily extracted by ethanol precipitation with a high yield of 7.5 g/l. It was mainly composed of 83.1% carbohydrates and 10.6% proteins. The flocculating activity revealed 97.6 ± 0.6% for kaolin suspension at 12.8 mg/l extracted flocculants from strain BF1 and 2.5 g/l CaCl2. Inter-estingly, the flocculating activity was 78% without the addition of metal ions. Furthermore, flocculants of strain BF1 can be effectively applied in the treatment of cassava starch wastewater and municipal wastewater.

Keywords

Cassava Starch, Exopolysaccharide, Floccu-Lants, Klebsiella variicola, Wastewater Treatment.
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  • Production of Novel Bio-Flocculants from Klebsiella variicola BF1 using Cassava Starch Wastewater and its Application

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Authors

Ngoc Tuan Nguyen
Toxicology and Biomedicine Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam
Thi Ha My Phan
Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, Province of China
Tuyet Nhung Tran
Ton Duc Thang University, Ho Chi Minh City, Viet Nam
Bharath Kumar Velmurugan
Toxicology and Biomedicine Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam
Rudolf Kiefer
Ton Duc Thang University, Ho Chi Minh City, Viet Nam

Abstract


In this study, Klebsiella variicola BF1 that uses cassa-va starch wastewater to produce flocculants was identified using 16S rDNA gene sequencing. The pure flocculants of strain BF1 could be easily extracted by ethanol precipitation with a high yield of 7.5 g/l. It was mainly composed of 83.1% carbohydrates and 10.6% proteins. The flocculating activity revealed 97.6 ± 0.6% for kaolin suspension at 12.8 mg/l extracted flocculants from strain BF1 and 2.5 g/l CaCl2. Inter-estingly, the flocculating activity was 78% without the addition of metal ions. Furthermore, flocculants of strain BF1 can be effectively applied in the treatment of cassava starch wastewater and municipal wastewater.

Keywords


Cassava Starch, Exopolysaccharide, Floccu-Lants, Klebsiella variicola, Wastewater Treatment.

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DOI: https://doi.org/10.18520/cs%2Fv117%2Fi1%2F121-129