Current Science

Open Access Open Access  Restricted Access Subscription Access

Production of Novel Bio-Flocculants from Klebsiella variicola BF1 using Cassava Starch Wastewater and its Application


Affiliations
1 Toxicology and Biomedicine Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam
2 Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, Province of China
3 Ton Duc Thang University, Ho Chi Minh City, Viet Nam
 

In this study, Klebsiella variicola BF1 that uses cassa-va starch wastewater to produce flocculants was identified using 16S rDNA gene sequencing. The pure flocculants of strain BF1 could be easily extracted by ethanol precipitation with a high yield of 7.5 g/l. It was mainly composed of 83.1% carbohydrates and 10.6% proteins. The flocculating activity revealed 97.6 ± 0.6% for kaolin suspension at 12.8 mg/l extracted flocculants from strain BF1 and 2.5 g/l CaCl2. Inter-estingly, the flocculating activity was 78% without the addition of metal ions. Furthermore, flocculants of strain BF1 can be effectively applied in the treatment of cassava starch wastewater and municipal wastewater.

Keywords

Cassava Starch, Exopolysaccharide, Floccu-Lants, Klebsiella variicola, Wastewater Treatment.
User
Notifications
Font Size

  • Buthelezi, S. P., Olaniran, A. O. and Pillay, B., Production and characterization of bioflocculants from bacteria isolated from wastewater treatment plant in South Africa. Biotechnol. Biopro-cess Eng., 2010, 15, 874–881.

  • Dermlim, W., Prasertsan, P. and Doelle, H., Screening and charac-terization of bioflocculant produced by isolated Klebsiella sp. Appl. Microbiol. Biotechnol., 1999, 52, 698–703.

  • Sun, P., Hui, C., Bai, N., Yang, S., Wan, L., Zhang, Q. and Zhao, Y., Revealing the characteristics of a novel bioflocculant and its flocculation performance in Microcystis aeruginosa removal. Sci. Rep., 2015, 5, 17465.

  • Yu, L. et al., A novel Fe(III) dependent bioflocculant from Kleb-siella oxytoca GS-4-08: culture conditions optimization and floc-culation mechanism. Sci. Rep., 2016, 6, 34980.

  • Liu, J. W., Ma, J. W., Liu, Y. Z., Yang, Y., Yue, D. B. and Wang, H. T., Optimized cultivation of a bioflocculant M-C11 produced by Klebsiella pneumoniae and its application in sludge dewater-ing. Huan jing ke xue=Huanjing kexue, 2014, 35, 1183–1190.

  • Luo, Z., Chen, L., Chen, C., Zhang, W., Liu, M., Han, Y. and Zhou, J., Production and characteristics of a bioflocculant by Klebsiella pneumoniae YZ-6 isolated from human saliva. Appl. Biochem. Biotechnol., 2014, 172, 1282–1292.

  • Mandal, A. K., Yadav, K. K., Sen, I. K., Kumar, A., Chakraborti, S., Islam, S. S. and Chakraborty, R., Partial characterization and flocculating behavior of an exopolysaccharide produced in nutrient-poor medium by a facultative oligotroph Klebsiella sp. PB12. J. Biosci. Bioeng., 2013, 115, 76–81.

  • Zhao, H., Liu, H. and Zhou, J., Characterization of a bioflocculant MBF-5 by Klebsiella pneumoniae and its application in acanth-moeba cysts removal. Bioresour. Technol., 2013, 137, 226–232.

  • Xing, J., Yang, J. X., Li, A., Ma, F., Liu, K. X., Wu, D. and Wei, W., Removal efficiency and mechanism of sulfamethoxazole in aqueous solution by bioflocculant MFX. J. Anal. Meth. Chem., 2013, 2013, 568614.

  • Ghosh, M., Pathak, S. and Ganguli, A., Effective removal of cryp-tosporidium by a novel bioflocculant. Water Environ. Res., 2009, 81, 160–164.

  • Wang, S. G., Gong, W. X., Liu, X. W., Tian, L., Yue, Q. Y. and Gao, Q. Y., Production of a novel bioflocculant by culture of Klebsiella mobilis using dairy wastewater. Biochem. Eng. J., 2007, 36, 15.

  • Yue, L., Ma, C. and Chi, Z., Bioflocculant produced by Klebsiella sp. MYC and its application in the treatment of oil-field produced water. J. Ocean Univ. China, 2006, 5, 333–338.

  • Nakata, K. and Kurane, R., Production of an extracellular polysac-charide bioflocculant by Klebsiella pneumoniae. Biosci., Biotech-nol. Biochem., 1999, 63, 2064–2068.

  • Xia, X., Liang, Y., Lan, S., Li, X., Xie, Y. and Yuan, W., Produc-tion and flocculating properties of a compound biopolymer floccu-lant from corn ethanol wastewater. Bioresour. Technol., 2018, 247, 924–929.

  • Deng, S. B., Bai, R. B., Hu, X. M. and Luo, Q., Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment. Appl. Microbiol. Biotechnol., 2003, 60, 588–593.

  • AOAC Official Methods of Analysis, Association of Official Ana-lytical Chemists, Arlington, 1990, 15th edn.

  • Lin, L. et al., Complete genome sequence of endophytic nitrogen-fixing Klebsiella variicola strain DX120E. Stand. Genomic Sci., 2015, 10, 22.

  • Nie, M. et al., Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment. J. Appl. Microbiol., 2011, 111, 547–558.

  • Li, Q., Liu, H. L., Qi, Q. S., Wang, F. S. and Zhang, Y. Z., Isola-tion and characterization of temperature and alkaline stable bioflocculant from Agrobacterium sp. M-503. New Biotechnol., 2010, 27, 789–794.

  • Zhang, J., Liu, Z., Wang, S. and Jiang, P., Characterization of a bioflocculant produced by the marine myxobacterium Nannocystis sp. NU-2. Appl. Microbiol. Biotechnol., 2002, 59, 517–522.

  • Shih, I. L., Van, Y. T., Yeh, L. C., Lin, H. G. and Chang, Y. N., Production of a biopolymer flocculant from Bacillus licheniformis and its flocculation properties. Bioresour. Technol., 2001, 78, 267–272.

  • Subudhi, S., Batta, N., Pathak, M., Bisht, V., Devi, A., Lal, B. and Alkhulifah, B., Bioflocculant production and biosorption of zinc and lead by a novel bacterial species, Achromobacter sp. TERI-IASST N, isolated from oil refinery waste. Chemosphere, 2014, 113, 116–124.

  • Fujita, M., Ike, M., Tachibana, S., Kitada, G., Kim, S. M. and Inoue, Z., Characterization of a bioflocculant produced by Citrobacter sp. TKF04 from acetic and propionic acids. J. Biosci. Bioeng., 2000, 89, 40–46.

  • El-Salam, A. E., Abd-El-Haleem, D., Youssef, A. S., Zaki, S., Abu-Elreesh, G. and El-Assar, S. A., Isolation, characterization, optimization, immobilization and batch fermentation of biofloccu-lant produced by Bacillus aryabhattai strain PSK1. J. Genet. Eng. Biotechnol., 2017, 15, 335–344.

  • Guo, H., Hong, C., Zheng, B., Lu, F., Jiang, D. and Qin, W., Bio-flocculants’ production in a biomass-degrading bacterium using untreated corn stover as carbon source and use of bioflocculants for microalgae harvest. Biotechnol. Biofuels, 2017, 10, 306.

  • Liu, W., Wang, K., Li, B., Yuan, H. and Yang, J., Production and characterization of an intracellular bioflocculant by Chryseobacte-rium daeguense W6 cultured in low nutrition medium. Bioresour. Technol., 2010, 101, 1044–1048.

  • Guo, J., Yang, C. and Peng, L., Preparation and characteristics of bacterial polymer using pre-treated sludge from swine wastewater treatment plant. Bioresour. Technol., 2014, 152, 490–498.

  • Liu, W. J., Yuan, H. L. and Yang, J. S., The mechanism of kaolin clay flocculation by a cation-independent bioflocculant produced by Chryseobacterium daeguense W6. AIMS Environ. Sci., 2015, 2, 169–179.

  • Li, Y., Xu, Y., Zheng, T. and Wang, H., Flocculation mechanism of the actinomycete Streptomyces sp. Hsn06 on chlorella vulgaris. Bioresour. Technol., 2017, 239, 137–143.

  • Nontembiso, P., Sekelwa, C., Leonard, M. V. and Anthony, O. I., Assessment of bioflocculant production by Bacillus sp. Gilbert, a marine bacterium isolated from the bottom sediment of algoa bay. Marine Drugs, 2011, 9, 1232–1242.

  • Zheng, Y., Ye, Z. L., Fang, X. L., Li, Y. H. and Cai, W. M., Pro-duction and characteristics of a bioflocculant produced by Bacillus sp. F19. Bioresour. Technol., 2008, 99, 7686–7691.


Abstract Views: 273

PDF Views: 81




  • Production of Novel Bio-Flocculants from Klebsiella variicola BF1 using Cassava Starch Wastewater and its Application

Abstract Views: 273  |  PDF Views: 81

Authors

Ngoc Tuan Nguyen
Toxicology and Biomedicine Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam
Thi Ha My Phan
Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, Province of China
Tuyet Nhung Tran
Ton Duc Thang University, Ho Chi Minh City, Viet Nam
Bharath Kumar Velmurugan
Toxicology and Biomedicine Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam
Rudolf Kiefer
Ton Duc Thang University, Ho Chi Minh City, Viet Nam

Abstract


In this study, Klebsiella variicola BF1 that uses cassa-va starch wastewater to produce flocculants was identified using 16S rDNA gene sequencing. The pure flocculants of strain BF1 could be easily extracted by ethanol precipitation with a high yield of 7.5 g/l. It was mainly composed of 83.1% carbohydrates and 10.6% proteins. The flocculating activity revealed 97.6 ± 0.6% for kaolin suspension at 12.8 mg/l extracted flocculants from strain BF1 and 2.5 g/l CaCl2. Inter-estingly, the flocculating activity was 78% without the addition of metal ions. Furthermore, flocculants of strain BF1 can be effectively applied in the treatment of cassava starch wastewater and municipal wastewater.

Keywords


Cassava Starch, Exopolysaccharide, Floccu-Lants, Klebsiella variicola, Wastewater Treatment.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi1%2F121-129