In this study, Klebsiella variicola BF1 that uses cassa-va starch wastewater to produce flocculants was identified using 16S rDNA gene sequencing. The pure flocculants of strain BF1 could be easily extracted by ethanol precipitation with a high yield of 7.5 g/l. It was mainly composed of 83.1% carbohydrates and 10.6% proteins. The flocculating activity revealed 97.6 ± 0.6% for kaolin suspension at 12.8 mg/l extracted flocculants from strain BF1 and 2.5 g/l CaCl2. Inter-estingly, the flocculating activity was 78% without the addition of metal ions. Furthermore, flocculants of strain BF1 can be effectively applied in the treatment of cassava starch wastewater and municipal wastewater.
Keywords
Cassava Starch, Exopolysaccharide, Floccu-Lants, Klebsiella variicola, Wastewater Treatment.
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