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Soybean Methylation Analysis during Strontium Stress using Methylation-Sensitive Amplified Polymorphism


Affiliations
1 Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
 

The effect of strontium stress on the pattern and degree of DNA methylation in soybean seedlings was analysed using the methylation-sensitive amplified polymorphism (MSAP) method. The growth traits were inhibited by SrCl2 treatments. A total of 167 loci were determined and evaluated for DNA methylation after different treatments. The level of cytosine methylation initially decreased and then increased with increasing Sr concentration. Methylation was lowest after 10 mmol/l SrCl2 treatment. Strontium stress resulted in a 57.48% alteration of DNA methylation patterns in 5′-CCGG-3′ loci. The pattern variation initially decreased and then increased along with increasing strontium concentration. There was a positive correlation between the total methylation and full methylation induced by strontium stress, and weight and length of shoots and ischolar_mains in soybean. Overall, the changes in the pattern and degree of methylation may be a key regulatory mechanism for soybean adaptation to strontium.

Keywords

Methylation/Demethylation, Polymorphism, Soybean, Strontium Stress.
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  • Soybean Methylation Analysis during Strontium Stress using Methylation-Sensitive Amplified Polymorphism

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Authors

Qingyuan He
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Zhengpeng Li
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Yingjie Shu
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Songhua Wang
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China
Shoucheng Huang
Life Science College of Anhui Science and Technology University, Fengyang, Auhui 233100, China

Abstract


The effect of strontium stress on the pattern and degree of DNA methylation in soybean seedlings was analysed using the methylation-sensitive amplified polymorphism (MSAP) method. The growth traits were inhibited by SrCl2 treatments. A total of 167 loci were determined and evaluated for DNA methylation after different treatments. The level of cytosine methylation initially decreased and then increased with increasing Sr concentration. Methylation was lowest after 10 mmol/l SrCl2 treatment. Strontium stress resulted in a 57.48% alteration of DNA methylation patterns in 5′-CCGG-3′ loci. The pattern variation initially decreased and then increased along with increasing strontium concentration. There was a positive correlation between the total methylation and full methylation induced by strontium stress, and weight and length of shoots and ischolar_mains in soybean. Overall, the changes in the pattern and degree of methylation may be a key regulatory mechanism for soybean adaptation to strontium.

Keywords


Methylation/Demethylation, Polymorphism, Soybean, Strontium Stress.

References





DOI: https://doi.org/10.18520/cs%2Fv117%2Fi3%2F486-491