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Green Synthesis of Silver Nanoparticles using Azadirachta Indica and Ocimum Sanctum Leaf Extract


Affiliations
1 Department of Biotechnology, Rama Devi Women’s University, Bhubaneswar 751 022, India
2 Department of Zoology, College of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
3 Department of Life Science, Rama Devi Women’s University, Bhubaneswar 751 022, India
 

In the present study, green synthesis of stable silver nanoparticles was done using methanolic leaf extract of Azadirachta indica L. as well as Ocimum sanctum L. The antimicrobial activity was screened against UTI causing bacteria, viz. Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus using disc diffusion method. Silver nanoparticles derived from tulsi leaf extract inhibited E. coli by 12.00 ± 0.65 mm, K. pneumoniae by 9.66 ± 0.11 mm and S. aureus by 23.00 ± 0.81 mm at concentrations 1 mg/ml. Similarly, silver nanoparticles derived from neem leaf extract inhibited K. pneumoniae by 9.11 ± 0.81 mm and S. aureus by 09.0 ± 1.17 mm at concentrations 1 mg/ml respectively. Lower concentration (1 mg/ml) of tulsi and higher concentration (4 mg/ml) of neem leaf methanolic extract was observed to be effective against urinary tract infections in human causative agent S. aureus.

Keywords

Antimicrobial Activity, Green Synthesis, Plant Extract, Silver Nanoparticle.
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  • Green Synthesis of Silver Nanoparticles using Azadirachta Indica and Ocimum Sanctum Leaf Extract

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Authors

Sonali Priyadarshini
Department of Biotechnology, Rama Devi Women’s University, Bhubaneswar 751 022, India
Sushree Sulava
Department of Zoology, College of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
Rasmita Bhol
Department of Life Science, Rama Devi Women’s University, Bhubaneswar 751 022, India
Somanatha Jena
Department of Biotechnology, Rama Devi Women’s University, Bhubaneswar 751 022, India

Abstract


In the present study, green synthesis of stable silver nanoparticles was done using methanolic leaf extract of Azadirachta indica L. as well as Ocimum sanctum L. The antimicrobial activity was screened against UTI causing bacteria, viz. Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus using disc diffusion method. Silver nanoparticles derived from tulsi leaf extract inhibited E. coli by 12.00 ± 0.65 mm, K. pneumoniae by 9.66 ± 0.11 mm and S. aureus by 23.00 ± 0.81 mm at concentrations 1 mg/ml. Similarly, silver nanoparticles derived from neem leaf extract inhibited K. pneumoniae by 9.11 ± 0.81 mm and S. aureus by 09.0 ± 1.17 mm at concentrations 1 mg/ml respectively. Lower concentration (1 mg/ml) of tulsi and higher concentration (4 mg/ml) of neem leaf methanolic extract was observed to be effective against urinary tract infections in human causative agent S. aureus.

Keywords


Antimicrobial Activity, Green Synthesis, Plant Extract, Silver Nanoparticle.

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DOI: https://doi.org/10.18520/cs%2Fv117%2Fi8%2F1300-1307