Green Synthesis of Silver Nanoparticles using Azadirachta Indica and Ocimum Sanctum Leaf Extract

In the present study, green synthesis of stable silver nanoparticles was done using methanolic leaf extract of Azadirachta indica L. as well as Ocimum sanctum L. The antimicrobial activity was screened against UTI causing bacteria, viz. Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus using disc diffusion method. Silver nanoparticles derived from tulsi leaf extract inhibited E. coli by 12.00 ± 0.65 mm, K. pneumoniae by 9.66 ± 0.11 mm and S. aureus by 23.00 ± 0.81 mm at concentrations 1 mg/ml. Similarly, silver nanoparticles derived from neem leaf extract inhibited K. pneumoniae by 9.11 ± 0.81 mm and S. aureus by 09.0 ± 1.17 mm at concentrations 1 mg/ml respectively. Lower concentration (1 mg/ml) of tulsi and higher concentration (4 mg/ml) of neem leaf methanolic extract was observed to be effective against urinary tract infections in human causative agent S. aureus.