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Introgression of Semi-Dwarf Gene in Kalanamak Rice using Marker-Assisted Selection Breeding


Affiliations
1 Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
 

Kalanamak is an important aromatic rice variety in India. Tall stature of Kalanamak causes lodging due to which its yield and other characters severely declines. Introgression of the semi-dwarfing gene (sd1) from CSR10 was performed with the help of markerassisted breeding. Backcross-derived plants were characterized for semi-dwarf nature. Improved Kalanamak lines were analysed for the sd1 gene and to check the presence of aroma, sensory analysis test and amplification with betaine aldehyde dehydrogenase 2 (badh 2) derived primer was performed. Improved versions of Kalanamak rice lines were either on par or superior in terms of yield, grain type and cooking quality with reduced height implicating the potentiality of marker-assisted backcross breeding for improvement of this rice variety.

Keywords

Aromatic Rice, Grain Quality, Lodging Resistance, Semi-Dwarf Gene.
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  • Singh, R. K., Singh, U. S., Khush, G. S., Rohilla, R., Singh, J. P., Singh, G. and Shekhar, K. H., Small and medium grained aromatic rices of India. Aromatic Rice Science, Enfield Publishers Inc, USA, and Oxford and IBH Publishing Co, New Delhi, 2005, pp.155–177.

  • Spiemeyer, W., Ellis, M. H. and Chandler, P. M., Semidwarf (sd-1), ‘green revolution’ rice, contains a defective gibberellin 20oxidase gene. Proc. Natl. Acad., USA, 2002, 99, 9043–9048.

  • Lin, Y. R. et al., Mapping of quantitative trait loci for plant height and heading date in two inter-sub specific crosses of rice and comparison across Oryza genus. Bot. Stud., 2011, 52, 1–14.

  • Acquino, R. C. and Jennings, P. R. Inheritance and significance of dwarfism in indica rice variety. Crop Sci., 1966, 6, 551–554.

  • Luo, Y., Zakaria, S., Basyah, B., Ma, T., Li, Z., Yang, J. and Yin, Z., Marker-assisted breeding of Indonesia local rice variety Siputeh for semi-dwarf phenotype, good grain quality and disease resistance to bacterial blight. Rice, 2014, 7, 33.

  • Ahn, S. N., Bollich, C. N. and Tanksley, S. D., RFLP tagging of a gene for aroma in rice. Theor. Appl. Genet., 1992, 84, 825–828.

  • Cordeiro, G. M., Christopher, M. J., Henry, R. J. and Reinke, R. F., Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence. Mol. Breed., 2002, 9, 245–20.

  • Bradbury, L. M. T., Fitzgerald, T. L., Henry, R. J., Jin, Q. and Waters, D. L. E., The gene for fragrance in rice. Plant Biotech. J., 2005, 3, 363–370.

  • Chen, S., Wu, J., Yang, Y., Shi, W. and Xu, M., The fgr gene responsible for rice fragrance was restricted within 69 kb. Plant Sci., 2006, 171, 505–514.

  • Buttery, R. G., Ling, L. C., Juliano, B. O. and Turnbaugh, J. G., Cooked rice aroma and 2-acetyl-1-pyrroline. J. Agric. Food Chem., 1983, 31, 823–826.

  • Rajpurohit, D. et al., Pyramiding of two bacterial blight resistance and a semidwarfing gene in type 3 Basmati using marker-assisted selection. Euphytica, 2011, 178, 111–126.

  • Murray, M. G. and Thompson, W. F., Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res., 1980, 8, 4321–4325.

  • Nagaraju, M., Mohanty, K. K., Chowdhury, D. and Gangadharan, C., A simple technique to detect scent in rice. Oryza, 1991, 28, 109–110.

  • Sheoran, O. P., Tonk, D. S., Kaushik, L. S., Hasija, R. C. and Pannu, R. S., Statistical software package for agricultural research workers. In Recent Advances in Information Theory, Statistics and Computer Applications (Hooda, D. S. and Hasija, R. C.), Department of Mathematics Statistics, CCS HAU, Hisar, 1998, pp. 139– 143.

  • Berner, D. K. and Hoff, B. J., Inheritance of scent in American long grain rice. Crop Sci., 1986, 26, 876–878.

  • Berry, P. M., Bradely, S., Pickett, R., Sterling, M., Baker, C. J. and Cameron, N., Lodging control through variety choice and management. In Proceedings of the Eighth HGCA R7D, Conference on Cereals and Oilseeds, Home Grown Cereals Authority, London, 2002, pp. 7.1–7.12.

  • Hirano, B. K., Ordonio, R. L. and Matsuoka , M., Engineering the lodging resistance mechanism of post-green revolution rice to meet future demands. Proc. Jap. Acad. Ser. B, 2017, 93, 220–233.

  • Katiyar, S., Verulkar, S., Chandel, G., Zhang, Y., Huang, B. and Bennet, J., Genetic analysis and pyramiding of two gall midge resistance genes (Gm2 and Gm6t) in rice (Oryza sativa L.). Euphytica, 2001, 122, 327–334.

  • Ramalingam, J., Basharat, H. S. and Zhang, G., STS and microsatellite marker-assisted selection for bacterial blight resistance and waxy genes in rice. Oryza sativa L. Euphytica, 2002, 127, 255–260.

  • Joseph, M., Gopalakrishnan, S., Sharma, R. K., Singh, V. P., Singh, A. K., Singh, N. K. and Mohapatra, T., Combining bacterial blight resistance and Basmati quality characteristics by phenotypic and molecular marker assisted selection in rice. Mol. Breed., 2004, 13, 377–387.

  • Zhang, J., Li, X., Jiang, G., Xu, Y. and He, Y. Q., Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice. Plant Breed., 2006, 125, 600–605.

  • Perumalsamy, S. et al., Functional marker-assisted selection for bacterial leaf blight resistance genes in rice (Oryza sativa L.). Plant Breed, 2009, 129, 400–406.

  • Gopala Krishnan, S. et al., Integrating marker assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breed., 2008, 127, 131–139.

  • Sundaram, R. M. et al., Introduction of bacterial blight resistance into Triguna, a high yielding, mid-early duration rice variety. Biotechnol. J., 2009, 4, 400–407.

  • Suh, J. P. et al., Development of elite breeding lines conferring Bph18 gene-derived resistance to brown plant hopper (BPH) by marker-assisted selection and genome-wide background analysis in japonica rice (Oryza sativa L.). Field Crop Res., 2011, 120, 215–222.

  • Lin, Y. R. et al., Mapping of quantitative trait loci for plant height and heading date in two inter-sub specific crosses of rice and comparison across Oryza genus. Bot. Stud., 2011, 52, 1–14.

  • Basavaraj, S. H. et al., Marker aided improvement of Pusa 6B, the maintainer parent of hybrid Pusa RH10, for resistance to bacterial blight. Ind. J. Genet. Plant Breed., 2009, 69, 10–16.

  • Basavaraj, S. H. et al., Marker-assisted improvement of bacterial blight resistance in parental lines of Pusa RH10, a superfine grain aromatic rice hybrid. Mol. Breed. 2010, 26, 293–305.

  • Perez, L. M., Redona, E. D., Mendioro, M. S., Vera Cruz, C. M. and Leung, H., Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica, 2009, 164, 627–636.

  • Zhou, Y. L. et al., Improvement of bacterial blight resistance of hybrid rice in China using the Xa23 gene derived from wild rice (Oryza rufipogon). Field Crop Res., 2011, 30, 637–644.

  • Hari, Y. et al., Marker-assisted improvement of a stable restorer line, KMR-3R and its derived hybrid KRH2 for bacterial blight resistance and grain quality. Plant Breed., 2011, 130, 608–616.

  • Nawarathna, R. N., Perera, A. L. T., Samarasinghe, W. L. G., Screening of BC1F1 population (BG 379-2/IR 07F102//BG 379-2) of rice (Oryza sativa L.) for submergence tolerance using molecular markers. J. Agric. Sci., 2014, 9, 154–156.

  • Yamamoto, T., Taguchi, S. F., Ukai, Y., Sasaki, T. and Yano, M., Mapping quantitative trait loci for days-to-heading, and culm, panicle internode lengths in a BC1F3 population using an elite rice variety, Koshihikari, as the recurrent parent. Breed. Sci., 2001, 51, 671.

  • Maeda, H., Ishii, T., Takamure, I., Kinoshita, T. and Kamijima, O., Molecular mapping of semidwarfing gene, sd-1, using RAPD and RFLP markers. Breed. Sci., 1995, 45, 93–95 (in Japanese).

  • Neeraja, C. N., Vemireddy, L. R., Malathi, S., Siddiq, E. A., Identification of alternate dwarfing gene sources to widely used DeeGee-Woo-Gen allele of sd-1 gene by molecular and biochemical assays in rice (Oryza sativa L.). Electron. J. Biotech., 2009, 12, 1– 11.

  • Luo, Y., Ma, T., Zhang, A., Ong, K. H., Li, Z., Yang, J. and Yin, Z., Marker-assisted breeding of Indonesia local rice variety Siputeh for semi-dwarf phenotype, good grain quality and disease resistance to bacterial blight. Rice, 2016, 9, 66.

  • Sasaki, A. et al., Green revolution: a mutant gibberellin-synthesis gene in rice. Nature, 2002, 416, 701–706.

  • Ashikari, M., Sasaki, A., Tanaka, M., Itoh, H. and Nishimura, A., Loss-of-function of a rice gibberellins biosynthetic gene, GA20 oxidase (GA20ox-2), led to the rice ‘green revolution’. Breed. Sci., 2002, 52, 143–150.

  • Kovi, M. R., Zhang, Y., Yu, S., Yang, G., Yan, W. and Xing, Y., Candidacy of a chitin-inducible gibberellins responsive gene for a major locus affecting plant height in rice that is closely linked to green revolution gene sd1. Theor. Appl. Genet., 2011, 123, 705– 714.

  • Bradbury, L. M. T., Gillies, S. A., Brushett, D. J.,Waters, D. L. E. and Henry, R. J., Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice. Plant Mol. Biol., 2008, 68, 439–449.

  • Shi, W. W., Yang, Y., Chen, S. H. and Xu, M. L., Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties. Mol. Breed., 2008, 22, 185–192.

  • Gaur, A., Shabir, W., Pandita, D., Bharti, N., Malav, A., Shikari, A. and Bhat, B., Understanding the fragrance in rice. J. Rice Res., 2016; http://dx.doi.org/10.4172/2375-4338.1000e125.

  • Wanchana, S., Kamolsukyunyong, W., Ruengphayak, S., Toojinda, T., Tragoonrung, S. and Vanavichit, A., A rapid construction of a physical contig across a 4.5 cM region for rice grain aroma facilitates marker enrichment for positional cloning. Sci. Asia, 2005, 31, 299–306.


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  • Introgression of Semi-Dwarf Gene in Kalanamak Rice using Marker-Assisted Selection Breeding

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Authors

Deepti Srivastava
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Md Shamim
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Anurag Mishra
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Prashant Yadav
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Deepak Kumar
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Pramila Pandey
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Nawaz A. Khan
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India
Kapildeo N. Singh
Department of Plant Molecular Biology and Genetic Engineering, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India

Abstract


Kalanamak is an important aromatic rice variety in India. Tall stature of Kalanamak causes lodging due to which its yield and other characters severely declines. Introgression of the semi-dwarfing gene (sd1) from CSR10 was performed with the help of markerassisted breeding. Backcross-derived plants were characterized for semi-dwarf nature. Improved Kalanamak lines were analysed for the sd1 gene and to check the presence of aroma, sensory analysis test and amplification with betaine aldehyde dehydrogenase 2 (badh 2) derived primer was performed. Improved versions of Kalanamak rice lines were either on par or superior in terms of yield, grain type and cooking quality with reduced height implicating the potentiality of marker-assisted backcross breeding for improvement of this rice variety.

Keywords


Aromatic Rice, Grain Quality, Lodging Resistance, Semi-Dwarf Gene.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi4%2F597-603