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Enhancing the Anti-Tyrosinase Activity of a Hypersaline Kitasatospora Sp. Sbsk430 by Optimizing the Medium Components


Affiliations
1 Department of Biotechnology, Goa University, Goa 403 206, India
 

Tyrosinase inhibitors from natural resources have been gaining importance in pharmaceutical and horticultural applications. A full factorial central composite design was used to study the interactive effect of three variables, i.e. D-mannitol, yeast extract and sodium chloride of the fermentation medium for maximizing anti-tyrosinase activity (75.5%) of a hypersaline actinobacteria, Kitasatospora sp. SBSK430. A quadratic model was found to fit the anti-tyrosinase activity (R2 = 0.948). Response surface analysis revealed that the optimum values of the medium components were 15 g/l D-mannitol, 5.6 g/l yeast extract and 1.2 g/l sodium chloride. Tyrosinase inhibition activity was enhanced 1.1-fold, using this approach.

Keywords

Actinobacteria, Anti-Tyrosinase, Fermentation Medium, Hypersaline, Kitasatospora sp.
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  • Enhancing the Anti-Tyrosinase Activity of a Hypersaline Kitasatospora Sp. Sbsk430 by Optimizing the Medium Components

Abstract Views: 298  |  PDF Views: 113

Authors

Michelle S. Fernandes
Department of Biotechnology, Goa University, Goa 403 206, India
Savita Kerkar
Department of Biotechnology, Goa University, Goa 403 206, India

Abstract


Tyrosinase inhibitors from natural resources have been gaining importance in pharmaceutical and horticultural applications. A full factorial central composite design was used to study the interactive effect of three variables, i.e. D-mannitol, yeast extract and sodium chloride of the fermentation medium for maximizing anti-tyrosinase activity (75.5%) of a hypersaline actinobacteria, Kitasatospora sp. SBSK430. A quadratic model was found to fit the anti-tyrosinase activity (R2 = 0.948). Response surface analysis revealed that the optimum values of the medium components were 15 g/l D-mannitol, 5.6 g/l yeast extract and 1.2 g/l sodium chloride. Tyrosinase inhibition activity was enhanced 1.1-fold, using this approach.

Keywords


Actinobacteria, Anti-Tyrosinase, Fermentation Medium, Hypersaline, Kitasatospora sp.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi4%2F649-653