Enhancing the Anti-Tyrosinase Activity of a Hypersaline Kitasatospora Sp. Sbsk430 by Optimizing the Medium Components

Tyrosinase inhibitors from natural resources have been gaining importance in pharmaceutical and horticultural applications. A full factorial central composite design was used to study the interactive effect of three variables, i.e. D-mannitol, yeast extract and sodium chloride of the fermentation medium for maximizing anti-tyrosinase activity (75.5%) of a hypersaline actinobacteria, Kitasatospora sp. SBSK430. A quadratic model was found to fit the anti-tyrosinase activity (R² = 0.948). Response surface analysis revealed that the optimum values of the medium components were 15 g/l D-mannitol, 5.6 g/l yeast extract and 1.2 g/l sodium chloride. Tyrosinase inhibition activity was enhanced 1.1-fold, using this approach.