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Limonene Attenuates Oxidative Stress and Extends Longevity in Caenorhabditis elegans


Affiliations
1 Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India
 

In traditional systems of medicine, plants belonging to the family Rutaceae have been used against ageing and associated debilitating changes. Considering the therapeutic potential of limonene (C10H16), the principal component of the Rutaceae family plants, the present study was designed to decipher lifespan and stress-modulating potential of monocyclic terpene limonene (LM) employing genetically tractable model system Caenorhabditis elegans. Furthermore, we tested oxidative stress tolerance and in vivo reactive oxygen species build-up, which was monitored with or without exposure to LM. Additionally, involvement of transcription factor DAF-16 was examined in terms of nuclear localization. Overall, this study has implications for developing future anti-ageing pharmacological strategies in the future.

Keywords

Ageing, Caenorhabditis elegans, Limonene, Oxidative Stress.
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  • Gems, D., Tragedy and delight: the ethics of decelerated ageing. Philos. Trans. R. Soc. London, Ser. B, 2011, 366(1561), 108–112.

  • Li, A. N., Li, S., Zhang, Y. J., Xu, X. R., Chen, Y. M. and Li, H. B., Resources and biological activities of natural polyphenols. Nutrients, 2014, 6(12), 6020–6047.

  • Singh, P. et al., Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, DLlimonene. Food Chem. Toxicol., 2010, 48(6), 1734–1740.

  • Brenner, S., The genetics of Caenorhabditis elegans. Genetics, 1974, 77(1), 71–94.

  • Pant, A. et al., Verminoside mediates life span extension and alleviates stress in Caenorhabditis elegans. Free Radic. Res., 2015, 49(11), 1384–1392.

  • Porta-de-la-Riva, M., Fontrodona, L., Villanueva, A. and Ceron, J., Basic Caenorhabditis elegans methods: synchronization and observation. J. Vis. Exp., 2012, 64, e4019.

  • Gandhi, S., Santelli, J., Mitchell, D. H., Stiles, J. W. and Sanadi, D. R., A simple method for maintaining large, ageing populations of Caenorhabditis elegans. Mech. Ageing Dev., 1980, 12(2), 137–150.

  • Garigan, D., Hsu, A. L., Fraser, A. G., Kamath, R. S., Ahringer, J. and Kenyon, C., Genetic analysis of tissue ageing in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation. Genetics, 2002, 161(3), 1101–1112.

  • Pant, A., Saikia, S. K., Shukla, V., Asthana, J., Akhoon, B. A. and Pandey, R., Beta-caryophyllene modulates expression of stress response genes and mediates longevity in Caenorhabditis elegans. Exp. Gerontol., 2014, 57, 81–95.

  • Adachi, H., Fujiwara, Y. and Ishii, N., Effects of oxygen on protein carbonyl and ageing in Caenorhabditis elegans mutants with long (age-1) and short (mev-1) lifespans. J. Gerontol. A Biol. Sci. Med. Sci., 1998, 53(4), 240–244.

  • Kenyon, C., A pathway that links reproductive status to lifespan in Caenorhabditis elegans. Ann. N. Y. Acad. Sci., 2010, 1204, 156–162.

  • Shukla, V., Yadav, D., Phulara, S. C., Gupta, M. M., Saikia, S. K. and Pandey, R., Longevity-promoting effects of 4-hydroxy-Eglobularinin in Caenorhabditis elegans. Free Radic. Biol. Med., 2012, 53(10), 1848–1856.

  • Schmittgen, T. D. and Livak, K. J., Analyzing real-time PCR data by the comparative C(T) method. Nature Protoc., 2008, 3(6), 1101–1108.

  • Chandrasekaran, A., Idelchik, M. D. and Melendez, J. A., Redox control of senescence and age-related disease. Redox. Biol., 2016, 11, 91–102.

  • Ogawa, T., Kodera, Y., Hirata, D., Blackwell, T. K. and Mizunuma, M., Natural thioallyl compounds increase oxidative stress resistance and lifespan in Caenorhabditis elegans by modulating SKN-1/Nrf. Sci. Rep., 2016, 6, 21611.

  • Lithgow, G. J., White, T. M., Melov, S. and Johnson, T. E., Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. Proc. Natl. Acad. Sci. USA, 1995, 92(16), 7540–7544.

  • Ogg, S. et al., The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature, 1997, 389(6654), 994–999.

  • Altintas, O., Park, S. and Lee, S. J., The role of insulin/IGF-1 signaling in the longevity of model invertebrates, C. elegans and D. melanogaster. BMB Rep., 2016, 49(2), 81–92.

  • Kimura, K. D., Tissenbaum, H. A., Liu, Y. and Ruvkun, G., daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. Science, 1997, 277(5328), 942–946.

  • Kenyon, C., The first long-lived mutants: discovery of the insulin/IGF-1 pathway for ageing. Philos. Trans. R. Soc. London, Ser. B, 2011, 366(1561), 9–16.

  • Avila, D. S., Benedetto, A., Au, C., Bornhorst, J. and Aschner, M., Involvement of heat shock proteins on Mn-induced toxicity in Caenorhabditis elegans. BMC Pharmacol. Toxicol., 2016, 17(1), 54–63.


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  • Limonene Attenuates Oxidative Stress and Extends Longevity in Caenorhabditis elegans

Abstract Views: 352  |  PDF Views: 127

Authors

Pooja Shukla
Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India
Aakanksha Pant
Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India
Rakesh Pandey
Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow 226 015, India

Abstract


In traditional systems of medicine, plants belonging to the family Rutaceae have been used against ageing and associated debilitating changes. Considering the therapeutic potential of limonene (C10H16), the principal component of the Rutaceae family plants, the present study was designed to decipher lifespan and stress-modulating potential of monocyclic terpene limonene (LM) employing genetically tractable model system Caenorhabditis elegans. Furthermore, we tested oxidative stress tolerance and in vivo reactive oxygen species build-up, which was monitored with or without exposure to LM. Additionally, involvement of transcription factor DAF-16 was examined in terms of nuclear localization. Overall, this study has implications for developing future anti-ageing pharmacological strategies in the future.

Keywords


Ageing, Caenorhabditis elegans, Limonene, Oxidative Stress.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi6%2F959-965