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Transcriptome Profile of Polycyclic Aromatic Hydrocarbon-Degrading Fungi Isolated from Taxus rhizosphere


Affiliations
1 School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
2 Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
 

The present study aims to examine polycyclic aromatic hydrocarbon (PAH)-degrading fungal strains isolated from rhizosphere soil of Taxus mairei. Talaromyces verruculosus strain DJTU-SJ5 showed the higher PAH degradation than other strains. Improved degradation of five PAHs was attained using the two-member consortium. The industrial potential of DJTU-SJ5 has been highlighted. The transcriptome profiles of DJTUSJ5 before and after PAH challenge were decoded by high-throughput RNA sequencing. A total of 33,084 unigenes were obtained by de novo assembly of 19.9 Gb clean sequencing reads; 10,532, 1,104 and 11,779 unigenes were expressed distinctly between control and phenanthrene (phen)-treated samples; control and pyrene (pyre)-treated samples, and phen and pyre respectively. These included numerous PAH degradation, defence and stress-related genes. The degradation pathway of PAHs in strain DJTU-SJ5 is proposed based on the metabolites and transcriptome analyses.

Keywords

Biodegradation, Polycyclic Aromatic Hydrocarbon, Talaromyces verruculosus, Taxus mairei, Transcriptome Sequencing.
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  • Transcriptome Profile of Polycyclic Aromatic Hydrocarbon-Degrading Fungi Isolated from Taxus rhizosphere

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Authors

Rui Gao
School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Da Cheng Hao
School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Wenli Hu
School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Song Song
School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China
Shi Yang Li
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Guang Bo Ge
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Abstract


The present study aims to examine polycyclic aromatic hydrocarbon (PAH)-degrading fungal strains isolated from rhizosphere soil of Taxus mairei. Talaromyces verruculosus strain DJTU-SJ5 showed the higher PAH degradation than other strains. Improved degradation of five PAHs was attained using the two-member consortium. The industrial potential of DJTU-SJ5 has been highlighted. The transcriptome profiles of DJTUSJ5 before and after PAH challenge were decoded by high-throughput RNA sequencing. A total of 33,084 unigenes were obtained by de novo assembly of 19.9 Gb clean sequencing reads; 10,532, 1,104 and 11,779 unigenes were expressed distinctly between control and phenanthrene (phen)-treated samples; control and pyrene (pyre)-treated samples, and phen and pyre respectively. These included numerous PAH degradation, defence and stress-related genes. The degradation pathway of PAHs in strain DJTU-SJ5 is proposed based on the metabolites and transcriptome analyses.

Keywords


Biodegradation, Polycyclic Aromatic Hydrocarbon, Talaromyces verruculosus, Taxus mairei, Transcriptome Sequencing.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi7%2F1218-1228