Current Science

Open Access Open Access  Restricted Access Subscription Access

Apoptosis Time Window Induced by Cold Atmospheric Plasma:Comparison with Ionizing Radiation


Affiliations
1 Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
2 Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
 

In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

Keywords

Apoptosis, Cold Atmospheric Plasma, Ionizing Radiation, Primary Human Fibroblasts, Repair Kinetics.
User
Notifications
Font Size

  • Downs, J. A., Lowndes, N. F. and Jackson, S. P., A role for Saccharomyces cerevisiae histone H2A in DNA repair. Nature, 2000, 408, 1001–1004.

  • Downs, J. A. et al., Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites. Mol. Cell, 2004, 16, 979–990.

  • Rogakou, E. P., Nieves-Neira, W., Boon, C., Pommier, Y. and Bonner, W. M., Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139. J. Biol. Chem., 2000, 275, 9390–9395.

  • Yuan, J., Adamski, R. and Chen, J., Focus on histone variant H2AX: to be or not to be. FEBS Lett., 2010, 584, 3717–3724.

  • Schipler, A. and Iliakis, G., DNA double-strand break complexity levels and their possible contributions to the probability for errorprone processing and repair pathway choice. Nucleic Acids Res., 2013, 41, 7589–7605.

  • Shibata, A. et al., Factors determining DNA double-strand break repair pathway choice in G2 phase. EMBO J., 2011, 30, 1079– 1092.

  • Dezest, M. et al., Mechanistic insights into the impact of cold atmospheric pressure plasma on human epithelial cell lines. Sci. Rep., 2017, 7, 41163.

  • Noda, A., Radiation-induced unrepairable DSBs: their role in the late effects of radiation and possible applications to biodosimetry. J. Radiat. Res., 2018, 59, ii114–ii120.

  • Bruggeman, P. J. et al., Plasma–liquid interactions: a review and roadmap. Plasma Sources Sci. Technol., 2016, 25, 053002.

  • Lukes, P., Dolezalova, E., Sisrova, I. and Clupek, M., Aqueousphase chemistry and bactericidal effects from an air discharge plasma in contact with water: evidence for the formation of peroxynitrite through a pseudo-second-order post-discharge reaction of H2O2 and HNO2. Plasma Sources Sci. Technol., 2014, 23, 015019.

  • Seth, A. N., Wei, T., Eric, J. and Mark, J. K., Atmospheric pressure plasma jets interacting with liquid covered tissue: touching and not-touching the liquid. J. Phys. D, 2014, 47, 475203.

  • Lazović, S. et al., Plasma induced DNA damage: comparison with the effects of ionizing radiation. Appl. Phys. Lett., 2014, 105, 124101.

  • Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S. and Bonner, W. M., DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Biol. Chem., 1998, 273, 5858– 5868.

  • Kinner, A., Wu, W., Staudt, C. and Iliakis, G., Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res., 2008, 36, 5678–5694.

  • Holmes, K. L., Otten, G. and Yokoyama, W. M., Flow cytometry analysis using the Becton Dickinson FACS Calibur. In Current Protocols in Immunology (eds Coligan, J. E. et al.), John Wiley, USA, 2002, vol. 49, pp. 5.4.1–5.4.22.

  • Janero, D. R., Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic. Biol. Med., 1990, 9, 515–540.

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., Protein measurement with the Folin phenol reagent. J. Biol. Chem., 1951, 193, 265–275.

  • Cook, P. J., Ju, B. G., Telese, F., Wang, X., Glass, C. K. and Rosenfeld, M. G., Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. Nature, 2009, 458, 591.

  • Xiao, A. et al., WSTF regulates the H2AX DNA damage response via a novel tyrosine kinase activity. Nature, 2009, 457, 57–62.

  • Fenech, M., The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat. Res., 1993, 285, 35–44.

  • Ayala, A., Munoz, M. F. and Arguelles, S., Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid. Med. Cell. Longev., 2014, 2014, 360438.

  • Haghdoost, S., Sjolander, L., Czene, S. and Harms-Ringdahl, M., The nucleotide pool is a significant target for oxidative stress. Free Radic. Biol. Med., 2006, 41, 620–626.

  • Vairetti, M., Ferrigno, A., Bertone, R., Richelmi, P., Berte, F. and Freitas, I., Apoptosis vs necrosis: glutathione-mediated cell death during rewarming of rat hepatocytes. Biochim. Biophys. Acta, 2005, 1740, 367–374.


Abstract Views: 307

PDF Views: 101




  • Apoptosis Time Window Induced by Cold Atmospheric Plasma:Comparison with Ionizing Radiation

Abstract Views: 307  |  PDF Views: 101

Authors

Gordana Joksic
Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
Ana Valenta Šobot
Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
Jelena Filipovic Trickovic
Department of Physical Chemistry, Vinca Institute of Nuclear Sciences, University of Belgrade, Mike Petrovica Alasa 12-14, 11001 Belgrade, Serbia
Dejan Maletic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Nevena Puac
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Gordana Malovic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Zoran Lj. Petrovic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
Sasa Lazovic
Institute of Physics, University of Belgrade, Pregrevica 118, 11080 Belgrade, Serbia

Abstract


In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4–1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2–3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h.

Keywords


Apoptosis, Cold Atmospheric Plasma, Ionizing Radiation, Primary Human Fibroblasts, Repair Kinetics.

References





DOI: https://doi.org/10.18520/cs%2Fv116%2Fi7%2F1229-1233