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Gelatin-Coated Magnetic Nanoparticles for Genomic DNA Isolation from Biological Samples


Affiliations
1 INBIOMEDIC Research and Technological Center, Jr Jose de la Torre Ugarte 166 Of. 102, Lince, Lima 15046, Peru, India
2 New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, United States
 

The selection of an appropriate DNA isolation method for a specific biological sample (whole blood, saliva, stool, urine, fresh tissue or paraffin-embedded tissue) is a prerequisite for any molecular testing. This selection is dependent on many factors, including yield, purity, time, safety, requirement of specialized equipment, trained personnel, intended downstream applications, cost and sample source.
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  • Borlido, L., Azevedo, A. M., Roque, A. C. A. and Aires-Barros, M. R., Biotechnol. Adv., 2013, 31, 1374–1385; doi:10.1016/j.biotechadv.2013.05.009.

  • Intorasoot, S., Srirung, R., Intorasoot, A. and Ngamratanapaiboon, S., Anal. Biochem., 2009, 386, 291–292; doi:10.1016/j.ab.2008.12.032.

  • Taylor, J. I., Hurst, C. D., Davies, M. J., Sachsinger, N. and Bruce, I. J., J. Chromatogr. A, 2000, 890, 159–166; doi:10.1016/S0021-9673(00)00107-2.

  • Yoza, B., Matsumoto, M. and Matsunaga, T., J. Biotechnol., 2002, 94, 217–224; doi:10.1016/S0168-1656(01)00427-8.

  • Chiang, C. L., Sung, C. S., Wu, T. F., Chen, C. Y. and Hsu, C. Y., J. Chromatogr. B, 2005, 822, 54–60; doi:10.1016/j.jchromb.2005.05.017.

  • Mehta, R. V., Upadhyay, R. V., Charles, S. W. and Ramchand, C. N., Biotechnol. Tech., 1997, 11, 493–496; doi:10.1023/ a:1018457915380.

  • Saiyed, Z. M., Ramchand, C. N. and Telang, S. D., J Phys. Condens. Matter., 2008, 20, 204153; doi:10.1088/09538984/20/20/204153.

  • Gaihre, B., Aryal, S., Khil, M. S. and Kim, H. Y., J. Microencapsul., 2008; doi:10.1080/02652040701737697.

  • Tungwiwat, W., Fucharoen, G., Ratanasiri, T., Sanchaisuriya, K. and Fucharoen, S., Clin. Chim. Acta, 2003, 334, 173–177; doi:10.1016/S00098981(03)00224-9.

  • Gunter, C. et al., Hum. Mol. Genet., 1998, 7, 1935–1946; doi:10.1093/hmg/ 7.12.1935.

  • Lo, Y. M. D., Patel, P., Sampietro, M., Gillmer, M. D. G., Fleming, K. A. and Wainscoat, J. S., Lancet, 1990, 335, 1463–1464; doi:10.1016/0140-6736(90)91491-R.


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  • Gelatin-Coated Magnetic Nanoparticles for Genomic DNA Isolation from Biological Samples

Abstract Views: 442  |  PDF Views: 115

Authors

Kelly S. Levano
INBIOMEDIC Research and Technological Center, Jr Jose de la Torre Ugarte 166 Of. 102, Lince, Lima 15046, Peru, India
Luis Jaramillo-valverde
INBIOMEDIC Research and Technological Center, Jr Jose de la Torre Ugarte 166 Of. 102, Lince, Lima 15046, Peru, India
Elizabeth Delgado
INBIOMEDIC Research and Technological Center, Jr Jose de la Torre Ugarte 166 Of. 102, Lince, Lima 15046, Peru, India
Sara Rose Guariglia
New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, United States
Heinner Guio
INBIOMEDIC Research and Technological Center, Jr Jose de la Torre Ugarte 166 Of. 102, Lince, Lima 15046, Peru, India

Abstract


The selection of an appropriate DNA isolation method for a specific biological sample (whole blood, saliva, stool, urine, fresh tissue or paraffin-embedded tissue) is a prerequisite for any molecular testing. This selection is dependent on many factors, including yield, purity, time, safety, requirement of specialized equipment, trained personnel, intended downstream applications, cost and sample source.

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DOI: https://doi.org/10.18520/cs%2Fv116%2Fi11%2F1794-1796