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An effective in vitro regeneration protocol is essential to improve the natural population of conservationprioritized plants species. The micropropagation techniques are considered cost-effective if the survival chance of tissue-cultured plants is excellent in field conditions. Comparative foliar micromorphometric characteristics were analysed in this study, to determine the sequential developmental adaptations of foliages of Oldenlandia herbacea plantlets under in vitro and field conditions. The leaf constants showed considerable variations in stomatal morphology, type and density (decreased from 60.0 to 40.75), vein islet density (increased from 8.3 to 13.5) and raphides density (increased from 20.9 to 36.0) in the foliage of tissue-cultured and field-transferred plantlets. The micromorphometric changes reflect the developmental improvements taking place in the greenhouse and field transplantation of O. herbacea plants, which are essential for the survival of plantlets under natural conditions.herbacea

Keywords

Foliar Micromorphology, In Vitro Regeneration, Medicinal Herb, Micropropagation, Oldenlandia herbacea.
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