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In vitro Antioxidant and Cytotoxic Capacity of Kappaphycus alvarezii Successive Extracts


Affiliations
1 School of Biosciences and Technology, VIT School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 632 014, India
2 Electrochemistry Research Group, Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
3 Chemistry of Heterocycles and Natural Product Research Laboratory, Department of Chemistry, School of Advanced Science, Vellore Institute of Technology, Vellore 632 014, India
 

Kappaphycus alvarezii, marine red algae was collected from the Mandapam coastal region, Tamil Nadu, India to examine cytotoxic and redox capacity of its extracts. HPLC analysis indicates the presence of phenolic compounds in the methanolic extract. The maximum phenol content measured as mg of gallic acid standard equivalent was found to be 86.45 ± 70.72 mg/ml extract. The total flavonoid content was found to be 85.52 ± 32.57 mg/ml in the extract (using quercetin as a standard). The antioxidant potential of the extracts was determined by employing DPPH scavenging assay and antioxidant assay using the phosphomolybdenum method. Methanolic extract demonstrated efficient antioxidant capacity and DPPH radical scavenging activity. GC–MS analysis was carried out in crude extracts of K. alvarezii. MTT assay showed that the extracts are toxic to HeLa cells and their IC50 value was 1.75 mg/ml. The study shows that different extracts of K. alvarezii have strong antioxidant and less cytotoxic activity against HELA cells.

Keywords

Antioxidants, Cytotoxic Capacity, Kappaphycus alvarezii, Phytochemical Screening.
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  • In vitro Antioxidant and Cytotoxic Capacity of Kappaphycus alvarezii Successive Extracts

Abstract Views: 372  |  PDF Views: 128

Authors

Ruthiran Papitha
School of Biosciences and Technology, VIT School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 632 014, India
Chinnadurai Immanuel Selvaraj
School of Biosciences and Technology, VIT School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 632 014, India
V. Palanichamy
School of Biosciences and Technology, VIT School of Agricultural Innovations and Advanced Learning, Vellore Institute of Technology, Vellore 632 014, India
Prabhakarn Arunachalam
Electrochemistry Research Group, Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Selvaraj Mohana Roopan
Chemistry of Heterocycles and Natural Product Research Laboratory, Department of Chemistry, School of Advanced Science, Vellore Institute of Technology, Vellore 632 014, India

Abstract


Kappaphycus alvarezii, marine red algae was collected from the Mandapam coastal region, Tamil Nadu, India to examine cytotoxic and redox capacity of its extracts. HPLC analysis indicates the presence of phenolic compounds in the methanolic extract. The maximum phenol content measured as mg of gallic acid standard equivalent was found to be 86.45 ± 70.72 mg/ml extract. The total flavonoid content was found to be 85.52 ± 32.57 mg/ml in the extract (using quercetin as a standard). The antioxidant potential of the extracts was determined by employing DPPH scavenging assay and antioxidant assay using the phosphomolybdenum method. Methanolic extract demonstrated efficient antioxidant capacity and DPPH radical scavenging activity. GC–MS analysis was carried out in crude extracts of K. alvarezii. MTT assay showed that the extracts are toxic to HeLa cells and their IC50 value was 1.75 mg/ml. The study shows that different extracts of K. alvarezii have strong antioxidant and less cytotoxic activity against HELA cells.

Keywords


Antioxidants, Cytotoxic Capacity, Kappaphycus alvarezii, Phytochemical Screening.



DOI: https://doi.org/10.18520/cs%2Fv119%2Fi5%2F790-798