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A New Modified Nutrient Media for Cultivation of Actinobacteria


Affiliations
1 Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, India
 

The growth of Actinobacteria was evaluated in a new modified nutrient agar media (MNA) supplemented with 1% sodium chloride (NaCl) and compared with the universally accepted media which are under regular use. Thirteen different Actinobacterial isolates were evaluated for their growth in both solid and liquid media in MNA along with four regularly used media ISP-2, kenknight, starch casein and nutrient media. Results indicated that eight isolates (Streptomyces bulli, Streptomyces griseorubens, Streptomyces viridobrunneus, Streptomyces A3, Streptomyces A7, Streptomyces A11, Streptomyces A12 and Streptomyces A13), out of thirteen isolates could grow significantly faster on MNA compared with other solid agar media. Similarly, growth in liquid media recorded by optical density (OD) indicated that three isolates (S. griseorubens, S. viridobrunneus and A7) showed significantly higher OD followed by ISP-2, nutrient, starch casein and kenknight broth after seven days of incubation. All the isolates except S. viridobrunneus and Streptomyces A12 could tolerate NaCl concentration upto 6% in liquid modified nutrient broth. The present study showed that Actinobacteria can be successfully grown faster with full pigmentation at 1% NaCl concentration and they can tolerate up to 5% NaCl concentration, in the new proposed MNA media.

Keywords

Actinobacteria, Modified Nutrient Agar Media, Optical Density, Sodium Chloride.
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  • Basu, S., Bose, C., Das, N., Das, D., Pal, M. and Khurana, S., Evolution of bacterial and fungal growth media. Bioinformation, 2015, 11, 182–184.

  • Wasas, A. D. et al., Use of Dorset egg medium for maintenance and transport of Neisseria menigitidis and Haemophilus influenzae type b. J. Clin. Microbiol., 1999, 37, 2045.

  • Widdel, F., Theory and measurement of bacterial growth. Grundpraktikum Mikrobiologie, 2010, 4, 1–11.

  • Cappuccino, J. C. and Sherman, N., Microbiology – A Laboratory Manual, Longman, USA, 1999, 4th edn.

  • Poovarasan, S., Mohandas, S., Panneerselvam, P., Saritha, B. and Ajay, K. M., Mycorrhizae colonizing actinomycetes promote plant growth and control bacterial blight disease of pomegranate (Punicagranatum L. cv Bhagwa). Crop Prot., 2013, 53, 175–181.

  • Mohandas, S., Poovarasan, S., Panneerselvam, P., Saritha, B., Upreti, K. K., Ranveerkamal and Sita, T., Guava (Psidiumguajava L.) rhizosphere Glomus mosseae spores harboractinomycetes with growth promoting and antifungal attributes. Sci. Hortic., 2013, 150, 371–376.

  • Lipman, C. B., Toxic and antagonistic effects of salts as related to ammonification by Bacillus subtiilis. Bot. Gaz., 1909, 48, 105– 1425.

  • MacLeod, R. A., Onofrey, E. and Norris, M. E., Nutrition and metabolism of marine bacteria. I. Survey of nutritional requirements. J. Bacteriol., 1954, 68(6), 680–686.

  • Sistrom, W. R., A requirement for sodium in the growth of Rhodopseudomonas spheroids. J. Gen. Microbiol., 1960, 22, 778–785.

  • Holm, G. E. and Sherman, J. M., Salt effects in bacterial growth. I. Preliminary investigation. J. Bacteriol., 1921, 6(6), 511–519.

  • Allen, M. B., The cultivation of Myxophyceae. Arch. Microbiol., 1952, 17, 34–53.

  • Kratz, W. A. and Myers, J., Nutrition and growth of several bluegreen algae. Am. J. Bot., 1955, 42, 282–287.

  • Gerloff, G. C., Fitzgerald, G. P. and Skoog, F., The minimal nutrition of Microcystis aeruginosa. Am. J. Bot., 1952, 39, 26–32.

  • Reddy, N. G., Ramakrishna, D. P. N. and Raja Gopal, S. V., A morphological, physiological and biochemical studies of marine Streptomyces rochei (MTCC 10109) showing antagonistic activity against selective human pathogenic microorganisms. Asian J. Biol. Sci., 2011, 4, 1–14.

  • Sultan, M. Z., Khatune, N. A., Sathi, Z. S., Bhuiyan, M. S. A. and Sadik, G. M., In vitro antibacterial activity of an active metabolite isolated from Streptomyces species. Biotechnology (Faisalabad), 2002, 1, 100–106.

  • Bhave, S. V., Shanbhag, P. V., Sonawane, S. K., Parab, R. R. and Mahajan, G. B., Isolation and characterization of halotolerant Streptomyces radiopugnans from Antarctica soil. Letts. Appl. Microbiol., 2013, 56, 348–355.

  • Manal, J. K., A new medium for the isolation and enrichment of halophilic actinobacteria. Life Sci. J., 2016, 13(1), 65–71.


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  • A New Modified Nutrient Media for Cultivation of Actinobacteria

Abstract Views: 344  |  PDF Views: 129

Authors

A. N. Ganeshamurthy
Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, India
B. Saritha
Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, India
T. K. Radha
Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, India
P. Panneerselvam
Division of Soil Science, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru 560 089, India

Abstract


The growth of Actinobacteria was evaluated in a new modified nutrient agar media (MNA) supplemented with 1% sodium chloride (NaCl) and compared with the universally accepted media which are under regular use. Thirteen different Actinobacterial isolates were evaluated for their growth in both solid and liquid media in MNA along with four regularly used media ISP-2, kenknight, starch casein and nutrient media. Results indicated that eight isolates (Streptomyces bulli, Streptomyces griseorubens, Streptomyces viridobrunneus, Streptomyces A3, Streptomyces A7, Streptomyces A11, Streptomyces A12 and Streptomyces A13), out of thirteen isolates could grow significantly faster on MNA compared with other solid agar media. Similarly, growth in liquid media recorded by optical density (OD) indicated that three isolates (S. griseorubens, S. viridobrunneus and A7) showed significantly higher OD followed by ISP-2, nutrient, starch casein and kenknight broth after seven days of incubation. All the isolates except S. viridobrunneus and Streptomyces A12 could tolerate NaCl concentration upto 6% in liquid modified nutrient broth. The present study showed that Actinobacteria can be successfully grown faster with full pigmentation at 1% NaCl concentration and they can tolerate up to 5% NaCl concentration, in the new proposed MNA media.

Keywords


Actinobacteria, Modified Nutrient Agar Media, Optical Density, Sodium Chloride.

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DOI: https://doi.org/10.18520/cs%2Fv120%2Fi4%2F718-722