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Biodegradation Kinetics of Acenaphthene by Sphingobacterium Sp. Strain RTSB Isolated from a Petroleum-contaminated Soil


Affiliations
1 Department of Chemistry, Sreegopal Banerjee College, Bagati, Magra, Hooghly 712 148, India
 

Acenaphthene metabolism was previously reported by a Gram-negative Sphingobacterium sp. strain RTSB. The degradation pathway was found to proceed involving the key intermediate 1-naphthoic acid and processed via trans-3-carboxy-2-hydroxybenzylidenepyruvic acid to salicylic acid and catechol. The present article demonstrates the biodegradation kinetics of acenaphthene along with the fate of some of the major metabolites, 1-naphthoic acid, 1-acenaphthenol, 1-acenaphthenone, acenaphthenequinone, salicylic acid, and catechol; where, the maximum accumulated amount of 1-naphthoic acid measured was 302.1 mg l–1 during the mid-log stage of growth. The degradation of acenaphthene was observed to follow a first order kinetic model with a rate constant 0.528 d–1 and halflife of 1.31 days.

Keywords

Acenaphthene, Biodegradation, Kinetic Study, 1-Naphthoic Acid, Sphingobacterium Sp.
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  • Marston, C. P., Pereira, C., Ferguson, J., Fischer, L., Hedstrom, O., Dashwood, W. M. and Barid, W. M., Effect of a complex environmental mixture from coal tar containing polycyclic aromatic hydrocarbons (PAH) on tumor initiation, PAH-DNA binding and metabolic activation of carcinogenic PAH in mouse epidermis. Carcinogenesis, 2001, 22, 1077–1086.

  • Mastrangelo, G., Fadda, E. and Marzia, V., Polycyclic aromatic hydrocarbons and cancer in man. Environ. Health Perspect., 1996, 104, 1166–1170.

  • Xue, W. and Warshawsky, D., Metabolic activation of polycyclic and heterocyclic aromatic hydrocarbons and DNA damage: a review. Toxicol. Appl. Pharmacol., 2005, 206, 73–93.

  • Habe, H. and Omori, T., Genetics of polycyclic aromatic hydrocarbon metabolism in diverse aerobic bacteria. Biosci. Biotechnol. Biochem., 2003, 67, 225–243.

  • Kanaly, R. A. and Harayama, S., Biodegradation of high molecular weight polycyclic aromatic hydrocarbons by bacteria. J. Bacteriol., 2000, 182, 2059–2067.

  • Pothuluri, J. V. and Cerniglia, C. E., Microbial metabolism of polycyclic aromatic hydrocarbons. In Biological Degradation and Bioremediation of Toxic Chemicals (ed. Chaudhry, R. G.), Chapman & Hall, London, 1994, pp. 92–124.

  • Shuttleworth, K. L. and Cerniglia, C. E., Environmental aspects of PAH biodegradation. Appl. Biochem. Biotechnol., 1995, 54, 291– 302.

  • Tortella, G. R., Diez, M. C. and Duran, N., Fungal diversity and use in decomposition of environmental pollutants. Crit. Rev. Microbiol., 2005, 31, 197–212.

  • Keith, L. H. and Telliard, W. A., Priority pollutants I-a perspective view. Environ. Sci. Technol., 1979, 13, 416-423.

  • Schocken, M. J. and Gibson, D. T., Bacterial oxidation of polycyclic aromatic hydrocarbons acenaphthene and acenaphthylene. Appl. Environ. Microbiol., 1984, 48, 10–16.

  • Windholz, M. et al., An encyclopedia of chemicals, drugs, and biological. In The Merck Index (eds O’Neil, M. J. et al.), Merck and Co. Inc., New Jersey, USA, 1983, 10th edn.

  • Chapman, P. J., Degradation mechanisms. In Proceedings of the Workshop: Microbial Degradation of Pollutants in Marine Environments (eds Bourquin, A. W. and Pritchard, P. H.), Gulf Breeze, U.S. Environmental Protection Agency, 1979, pp. 28–66.

  • Ellis, B., Harold, P. and Kronberg, H., Bioremediation of a creosote contaminated site. Environ. Technol., 1991, 12, 447–459.

  • Geiselbrecht, A. D., Hedlund, B. P., Tichi, M. A. and Staley, J. T., Isolation of marine polycyclic aromatic hydrocarbon (PAH)degrading Cycloclasticus strains from the Gulf of Mexico and comparison of their PAH degradation ability with that of Puget Sound Cycloclasticus strains. Appl. Environ. Microbiol., 1998, 64, 4703–4710.

  • Ghosal, D., Dutta, A., Chakraborty, J., Basu, S. and Dutta, T. K., Characterization of the metabolic pathway involved in assimilation of acenaphthene in Acinetobacter sp. strain AGAT-W. Res. Microbiol., 2013, 164, 155–163.

  • Hedlund, B. P., Geiselbrecht, A. D., Bair, T. J. and Staley, J. T., Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov. Appl. Environ. Microbiol., 1999, 65, 251–259.

  • Kafilzadeh, F. et al., Isolation and identification of carcinogen acenaphthene-degrading endemic bacteria from crude oil contaminated soils around abadan refinery. J. Fasa. Univ. Med. Sci., 2012, 2, 181–186.

  • Komatsu, T., Omori, T. and Kodama, T., Microbial degradation of polycyclic aromatic hydrocarbons acenaphthene and acenaphthylene by a pure bacterial culture. Biosci. Biotechnol. Biochem., 1993, 57, 864–865.

  • Mallick, S., Biodegradation of acenaphthene by Sphingobacterium sp. strain RTSB involving trans-3-carboxy-2-hydroxybenzylidenepyruvic acid as a metabolite. Chemosphere, 2019, 219, 748–755.

  • Pinyakong, O., Habe, H., Kouzuma, A., Nojiri, H., Yamane, H. and Omori, T., Isolation and characterization of genes encoding polycyclicaromatic hydrocarbon dioxygenase from acenaphthene and acenaphthylene degrading Sphingomonas sp. strain A4. FEMS Microbiol. Lett., 2004, 238, 297–305.

  • Salam, L. B., Obayori, O. S. and Hawa, O., Hydrocarbon degradation and biosurfactant production by an acenaphthene-degrading Pseudomonas species. Soil Sediment Contam., 2016, 25, 837–856.

  • Selifonov, S. A. et al., Isolation and characterization of (1)-1,1adihydroxy1-hydrofluoren-9-one formed by angular dioxygenation in the bacterial catabolism of fluorine. Biochem. Biophys. Res. Commun., 1993, 193, 67–76.

  • Shi, T., Fredrickson, J. K. and Balkwill, D. L., Biodegradation of polycyclic aromatic hydrocarbons by Sphingomonas strains isolated from the terrestrial subsurface. J. Ind. Microbiol. Biotechnol., 2001, 26, 283–289.

  • Mallick, S., A review on origin, occurrence and biodegradation of polycyclic aromatic hydrocarbon acenaphthene. Appl. Ecol. Environ. Sci., 2019, 7, 263–269.

  • Selifonov, S. A. et al., Oxidation of naphthenoaromatic and methyl-substituted aromatic compounds naphthalene-1,2-dioxygenase. Appl. Environ. Microbiol., 1996, 62, 507–514.

  • Lowry, O. H. et al., Protein measurement with the Folin phenol reagent. J. Biol. Chem., 1951, 193, 265–267.

  • Simkins, S. and Alexander, M., Models for mineralization kinetics with the variables of substrate concentration and population density. Appl. Environ. Microbiol., 1984, 47, 1299–1306.

  • Guerin, W. F. and Jones, G. E., Two-stage mineralization of phenanthrene by estuarine enrichment cultures. Appl. Environ. Microbiol., 1988, 54, 929–936.

  • Tian, L., Ma, P. and Zhong, J. J., Kinetics and key enzyme activities of phenanthrene degradation by a new phenanthrenedegrading strain Pseudomonas mendocina. Process Biochem., 2002, 37, 1431–1437.

  • Tian, L., Ma, P. and Zhong, J. J., Impact of the presence of salicylate or glucose on enzyme activity and phenanthrene degradation by Pseudomonas mendocina. Process Biochem., 2003, 38, 1125–1132.


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  • Biodegradation Kinetics of Acenaphthene by Sphingobacterium Sp. Strain RTSB Isolated from a Petroleum-contaminated Soil

Abstract Views: 247  |  PDF Views: 112

Authors

Somnath Mallick
Department of Chemistry, Sreegopal Banerjee College, Bagati, Magra, Hooghly 712 148, India

Abstract


Acenaphthene metabolism was previously reported by a Gram-negative Sphingobacterium sp. strain RTSB. The degradation pathway was found to proceed involving the key intermediate 1-naphthoic acid and processed via trans-3-carboxy-2-hydroxybenzylidenepyruvic acid to salicylic acid and catechol. The present article demonstrates the biodegradation kinetics of acenaphthene along with the fate of some of the major metabolites, 1-naphthoic acid, 1-acenaphthenol, 1-acenaphthenone, acenaphthenequinone, salicylic acid, and catechol; where, the maximum accumulated amount of 1-naphthoic acid measured was 302.1 mg l–1 during the mid-log stage of growth. The degradation of acenaphthene was observed to follow a first order kinetic model with a rate constant 0.528 d–1 and halflife of 1.31 days.

Keywords


Acenaphthene, Biodegradation, Kinetic Study, 1-Naphthoic Acid, Sphingobacterium Sp.

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DOI: https://doi.org/10.18520/cs%2Fv120%2Fi5%2F926-931