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Fate of stem cells grown on the extracellular matrix isolated from cancer cells and their possible applications in tissue engineering


Affiliations
1 Tissue Engineering and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603 103, India
 

Propagation of stem cells in abundance is essential for use as cell therapy in regenerative medicine. Proliferation and differentiation of stem cells are influenced by interaction between cells and their microenvironment. Extracelluar matrix (ECM) forms the chunk of niche with its components, which is remodelled by the cellular activity. The remodelling and change of ECM components have an impact on cellular activity. ECM plays a vital role in the uncontrolled proliferation of cancer cells. In the present study, we have examined the proliferative ability of mouse embryonic stem cells (C3H10 T1/2 clone 8) grown on cell-free ECM isolated from KB and MCF-7 cancer cell line cultures, separately, using standard ammonium hydroxide method. The texture of ECM was characterized from images captured with inverted microscope and scanning electron microscope (SEM). The growth of C3H10 T1/2 clone 8 cells over KB-ECM and MCF-ECM was monitored up to 192 h and the doubling time was estimated. KB-ECM promoted growth rate by reducing the doubling time from 23 to 14 h, whereas MCF-ECM prolonged the lifetime of stem cells by extending the log phase of growth. The surface topography of KBECM under SEM showed rough, irregular and meshlike structure compared to MCF-7-derived ECM. This may account for enhanced growth rate of stem cells. The findings underscore the relevance of modifications to scale-up the generation of stem cells for use in regenerative medicine. Further studies are required with different sources of stem cells grown on modified components of ECM to identify the appropriate ECM.

Keywords

: Cancer cell lines, extracellular matrix, regenerative medicine, stem cells, tissue engineering.
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  • Haque, M. A., Nagaoka, M., Hexig, B. and Akaike, T., Artificial extracellular matrix for embryonic stem cell cultures: a new frontier of nanobiomaterials. Sci. Technol. Adv. Mater., 2010, 11, 1–10.

  • Lu, P., Weaver, V. M. and Werb, Z., The extracellular matrix: a dynamic niche in cancer progression. J. Cell Biol., 2012, 196, 395–406.

  • Ahmed, M. and Ffrench-Constant, C., Extracellular matrix regulation of stem cell behavior. Curr. Stem Cell Rep., 2016, 2, 197– 206.

  • Lutolf, M. P., Gilbert, P. M. and Blau, H. M., Designing materials to direct stem-cell fate. Nature, 2009, 462(7272), 433–441.

  • Gilbert, P. M. et al., Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture. Science, 2010, 329, 1078–1081.

  • Butcher, D., Alliston, T. and Weaver, V., A tense situation: forcing tumour progression. Nature Rev. Cancer, 2009, 9, 108–122.

  • Samuel, M. S. et al., Actomyosin-mediated cellular tension drives increased tissue stiffness and -catenin activation to induce epidermal hyperplasia and tumor growth. Cancer Cell, 2011, 19, 776–791.

  • Lopez, J. I., Kang, I., You, W.-K., McDonald, D. M. and Weaver, V. M., In situ force mapping of mammary gland transformation. Integr. Biol., 2011, 3, 910–921.

  • Wozniak, M. A., Desai, R., Solski, P. A., Der, C. J. and Keely, P. J., ROCK-generated contractility regulates breast epithelial cell differentiation in response to the physical properties of a threedimensional collagen matrix. J. Cell Biol., 2003, 163, 583–595.

  • Paszek, M. J. et al., Tensional homeostasis and the malignant phenotype. Cancer Cell, 2005, 8, 241–254.

  • Kronenberg, H. M., PTH regulates the hematopoietic stem cell niche in bone. Adv. Exp. Med. Biol., 2007, 602, 57–60.

  • Kasper, S., Exploring the origins of the normal prostate and prostate cancer stem cell. Stem Cell Rev., 2008, 4, 193–201.

  • Kim, D. H., Wong, P. K., Park, J., Levchenko, A. and Sun, Y., Microengineered platforms for cell mechanobiology. Annu. Rev. Biomed. Eng., 2009, 11, 203–233.

  • Battista, S. et al., The effect of matrix composition of 3D constructs on embryonic stem cell differentiation. Biomaterials, 2005, 26, 6194–6207.

  • Handorf, A. M., Zhou, Y., Halanski, M. A. and Li, W. J., Tissue stiffness dictates development, homeostasis, and disease progression. Organogenesis, 2015, 11(1), 1–15.

  • Hoch, A. I., Mittal, V., Mitra, D., Vollmer, N., Zikry, C. A. and Leach, J. K., Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells. Biomaterials, 2016, 74, 178–187.

  • Decaris, M. L., Binder, B. Y., Soicher, M. A., Bhat, A. and Leach, J. K., Cell-derived matrix coatings for polymeric scaffolds. Tissue Eng., 2012, 18(19–20), 2148–2157.

  • Harvestine, J. N., Vollmer, N. L., Ho, S. S., Zikry, C. A., Lee, M. A. and Leach, J. K., Extracellular matrix-coated composite scaffolds promote mesenchymal stem cell persistence and osteogenesis. Biomacromolecules, 2016, 17(11), 3524–3531.

  • Nair, G. G. et al., Recapitulating endocrine cell clustering in culture promotes maturation of human stem cell-derived cells. Nature Cell Biol., 2019, 21, 263–274.

  • Ghosh, R. and Girigoswami, K., NADH dehydrogenase subunits are overexpressed in cells exposed repeatedly to H2O2. Mutat. Res., 2008, 638, 210–215.

  • Hellewell, A. L., Rosini, S. and Adams, J. C., A rapid, scalable method for the isolation, functional study and analysis of cellderived extracellular matrix. J. Vis. Exp., 2017, 119, 55051.

  • Girigoswami, K., Ku, S. H., Ryu, J. and Park, C. B., A synthetic amyloid lawn system for high-throughput analysis of amyloid toxicity and drug screening. Biomaterials, 2008, 29(18), 2813–2819.

  • Girigoswami, K. B. and Ghosh, R., Response to irradiation in V79 cells conditioned by repeated treatment with low doses of hydrogen peroxide. Radiat. Environ. Biophys., 2005, 44(2), 131– 137.

  • Caplan, A. I., Why are MSCs therapeutic? New data: new insight. J. Pathol., 2009, 217(2), 318–324.

  • Groeber, F., Holeiter, M., Hampel, M., Hinderer, S. and SchenkeLayland, K., Skin tissue engineering – in vivo and in vitro applications. Adv. Drug Deliv. Rev., 2011, 63, 352–366.

  • Hinderer, S., Seifert, J., Votteler, M., Shen, N., Rheinlaender, J., Schäffer, T. E. and Schenke-Layland, K., Engineering of a biofunctionalized hybrid off-the-shelf heart valve. Biomaterials, 2014, 35, 2130–2139.

  • Wang, H. et al., Promotion of cardiac differentiation of brown adipose derived stem cells by chitosan hydrogel for repair after myocardial infarction. Biomaterials, 2014, 35, 3986–3998.

  • Monaghan, M., Browne, S., Schenke-Layland, K. and Pandit, A., A collagen-based scaffold delivering exogenous microRNA-29B to modulate extracellular matrix remodelling. Mol. Ther., 2014, 22(4), 786–796.

  • Oskarsson, T., Extracellular matrix components in breast cancer progression and metastasis. Breast, 2013, 22, S66–S72.

  • Lu, P., Takai, K., Weaver, V. M. and Werb, Z., Extracellular matrix degradation and remodeling in development and disease. Cold Spring Harbor Perspect. Biol., 2011, 3(12), a005058.

  • Nelson, C. M. and Bissell, M. J., Of extracellular matrix, scaffolds, and signaling: tissue architecture regulates development, homeostasis, and cancer. Annu. Rev. Cell Dev. Biol., 2006, 22, 287–309.

  • Rozario, T. and DeSimone, D. W., The extracellular matrix in development and morphogenesis: a dynamic view. Dev. Biol., 2010, 341(1), 126–140.

  • Chaudhuri, O. et al., Extracellular matrix stiffness and composition jointly regulate the induction of malignant phenotypes in mammary epithelium. Nature Mater., 2014, 13(10), 970–978.

  • Dalby, M. J., Gadegaard, N. and Oreffo, R. O., Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate. Nature Mater., 2014, 13(6), 558–569.

  • Anna, B., Dorota, P. and Krzysztof, R., Influence of extracellular matrix on the proliferation and adhesion properties of stem cells derived from different sources. Eur. J. Biol. Res., 2017, 7(3), 165–171.

  • Lai, Y. et al., Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells. Stem Cells Dev., 2010, 19, 1095–1107.

  • Lanfer, B., Seib, F. P., Freudenberg, U., Stamov, D., Bley, T., Bornhäuser, M. and Werner, C., The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices. Biomaterials, 2009, 30, 5950–5958.

  • Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. and Watson, J. D., Molecular Biology of the Cell (Third edition), Garland Publishing, New York, USA, 1994, p. 1361.


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  • Fate of stem cells grown on the extracellular matrix isolated from cancer cells and their possible applications in tissue engineering

Abstract Views: 386  |  PDF Views: 131

Authors

Koyeli Girigoswami
Tissue Engineering and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603 103, India
Devender
Tissue Engineering and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603 103, India
N. Srinivasan
Tissue Engineering and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603 103, India
Agnishwar Girigoswami
Tissue Engineering and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603 103, India

Abstract


Propagation of stem cells in abundance is essential for use as cell therapy in regenerative medicine. Proliferation and differentiation of stem cells are influenced by interaction between cells and their microenvironment. Extracelluar matrix (ECM) forms the chunk of niche with its components, which is remodelled by the cellular activity. The remodelling and change of ECM components have an impact on cellular activity. ECM plays a vital role in the uncontrolled proliferation of cancer cells. In the present study, we have examined the proliferative ability of mouse embryonic stem cells (C3H10 T1/2 clone 8) grown on cell-free ECM isolated from KB and MCF-7 cancer cell line cultures, separately, using standard ammonium hydroxide method. The texture of ECM was characterized from images captured with inverted microscope and scanning electron microscope (SEM). The growth of C3H10 T1/2 clone 8 cells over KB-ECM and MCF-ECM was monitored up to 192 h and the doubling time was estimated. KB-ECM promoted growth rate by reducing the doubling time from 23 to 14 h, whereas MCF-ECM prolonged the lifetime of stem cells by extending the log phase of growth. The surface topography of KBECM under SEM showed rough, irregular and meshlike structure compared to MCF-7-derived ECM. This may account for enhanced growth rate of stem cells. The findings underscore the relevance of modifications to scale-up the generation of stem cells for use in regenerative medicine. Further studies are required with different sources of stem cells grown on modified components of ECM to identify the appropriate ECM.

Keywords


: Cancer cell lines, extracellular matrix, regenerative medicine, stem cells, tissue engineering.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi10%2F1616-1622