Current Science

Open Access Open Access  Restricted Access Subscription Access

Gynaecological Disorders Associated with Brucella melitensis in Goat Flocks with Potential Risk of Occupational Zoonoses in Central India


Affiliations
1 College of Veterinary and Animal Sciences, Udgir 413 517, India
2 Nagpur Veterinary College, Nagpur 440 006, India
 

Brucellosis is an occupational infectious disease affecting both animals and humans. It causes abortions, retention of placenta, stillbirth and infertility in small and large ruminants. It is considered as a disease of economic importance to the society engaged in animal husbandry. The present study was aimed to estimate the prevalence of brucellosis in goats exhibiting abortions, retention of placenta and still births and in occupationally exposed individuals, to type the circulating Brucella species in Central India and risk factors for its spillage to human host. Seroprevalence of 34.72% (RBPT), 33.33% (SAT and S-LPS ELISA) was recorded in goats and 2.38% (RBPT, SAT and S-LPS ELISA) in occupationally exposed individuals. The clinical samples revealed three Brucella melitensis isolates as confirmed by bcsp31, IS711 and AMOS PCR. Early diagnosis, general awareness, restricted animal movement, hygiene, secure handling and disposal of infectious waste is warranted.

Keywords

Brucella melitensis, Goats, Gynaecological Disorders, Occupational Zoonoses, Seroprevalence.
User
Notifications
Font Size

  • World Health Organization, Brucellosis (human). Excerpt from WHO recommended standards and strategies for surveillance, prevention and control of communicable diseases, 2020, pp. 1–3; https://www.who.int/zoonoses/diseases/Brucellosissurveillance.pdf?ua=1 (accessed on 4 October 2020).

  • CDC, Brucellosis homepage, 2012; http://www.cdc.gov/brucellosis/veterinarians/host-animals.html (accessed on 4 April 2020).

  • de Bagüés, J., María, P., María, I., Arias, M. A., Julián, P., Axel, C. and Michel, S. Z., The new strains Brucella inopinata BO1 and Brucella species 83–210 behave biologically like classic infectious Brucella species and cause death in murine models of infection, J. Infect. Dis., 2014, 210(3), 467–472.

  • FAO, FAO Statistical Yearbook, 2014; http://www.fao.org/3/ai3590e.pdf (accessed on 4 April 2020).

  • Singh, S. V., Singh, N., Singh, M. P., Shankar, H. and Lalwani, D. D., Occurrence of abortions and seroprevalence of brucellosis in goats and sheep. Small Ruminant Res., 1994, 14(2), 161–165.

  • Gupta, V. K., Verma, D. K., Rout, P. K., Singh, S. V. and Vihan, V. S., Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Ruminant Res., 2006, 65(1–2), 79–84.

  • Gupta, V. K., Kumari, R., Verma, D. K., Singh, K., Singh, S. V. and Vihan, V. S., Detection of Brucella melitensis from goat tissues employing PCR. Indian J. Animal Sci., 2006, 76(10), 793–795.

  • Corbel, M. J., Food and Agriculture Organization of the United Nations, World Health Organization and World Organization for Animal Health, Brucellosis in humans and animals, 2006; https:// apps.who.int/iris/handle/10665/43597 (accessed on 26 August 2020).

  • OIE, Bovine brucellosis. In Manual of Standard for Diagnostics Tests and Vaccine for Terrestrial Animals, Office International des Epizooties, Paris, 2009, 5th edn.

  • Alton, G. G., Jones, L. M. and Pietz, D. E., Laboratory Techniques in Brucellosis, World Health Organization, Geneva, Switzerland, 1975, 2nd edn.

  • Muñoz, P. M. et al., Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of falsepositive serological results due to Yersinia enterocolitica O:9. Clin. Vaccine Immunol., 2005, 12(1), 141–151.

  • Bricker B. J. and Halling, S. M., Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol., 1994, 32(11), 2660–2666.

  • Baily, G. G., Krahn, J. B., Drasar, B. S. and Stoker, N. G., Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg., 1992, 95, 271–275.

  • Hénault, S., Calvez, D., Thiébaud, M., Boulière, M. and GarinBastuji, B., Validation of a nested-PCR based on the IS6501/711 sequence for the detection of Brucella in animal samples. In Proceedings of the Brucellosis 2000 International Research Conference (including the 53rd Brucellosis Research Conference), Nîmes, France, 2000, p. 45.

  • Sonekar, C. P. et al., Brucellosis in migratory sheep flock from Maharashtra, India. Trop. Anim. Health Product., 2018, 50(1), 91– 96.

  • Awandkar, S. P., Gosawi, P. P., Khode, N. V., Jadhao, S. G., Mendhe, M. S. and Kulkarni, M. B., Seroepidemiology of Brucellosis in Deccani Sheep. Indian Veter. J., 2012, 89(6), 30–32.

  • Awandkar, S. P., Sardar, V. M., Jadhao, S. G., Khode, N. V. and Kulkarni, M. B., An evidence of brucella zoonoses. In First Annual Conference of SRL and NAWAR and Symposium on Concepts in Zoonoses and Health in New Millennium at Nagpur, India, 2015, p. 53.

  • Sadhu, D. B., Panchasara, H. H., Chauhan, H. C., Sutariya, D. R., Parmar, V. L. and Prajapati, H. B., Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants. Veter. World, 2015, 8(5), 561–566.

  • Saxena, N., Singh, B. B. and Saxena, H. M., Brucellosis in sheep and goats and its serodiagnosis and epidemiology. Int. J. Curr. Microbiol. Appl. Sci., 2018, 7(1), 1848–1877.

  • Leahy, E., Shome, R, Deka, P., Sahay, S., Grace, D., Mazeri, S. and Lindah, J. F., Risk factors for Brucella spp. and Coxiella burnetii infection among small ruminants in Eastern India. Inf. Ecol. Epidemiol., 2020, 10(1); https://doi.org/10.1080/20008686.2020.1783091.

  • Lone, I. M., Baba, M. A., Shah, M. M., Iqbal, A. and Sakina, A., Seroprevalence of brucellosis in sheep of organized and unorganized sector of Kashmir valley. Veter. World, 2013, 6(8), 530–533; doi:10.5455/vetworld.2013.530-533.

  • Kanani, A., Dabhi, S., Patel, Y., Chandra, V., Vinodh Kumar, O. R. and Shome, R., Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India. Veter. World, 2018, 11(8), 1030–1036.

  • Chand, P. and Chhabra, R., Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India. Trop. Anim. Health Product., 2013, 45, 1313–1319.

  • Shome, R. et al., Spatial seroprevalence of bovine brucellosis in India – A large random sampling survey. Comp. Immunol., Microbiol. Infect. Dis., 2019, 65, 124–127.

  • Prasad, S., New Paradigm in Livestock Production from Traditional to Commercial Farming and Beyond, Agrotech Publishing Academy, Udaipur, 2013, pp. 57–80.

  • Rossetti, C. A., Arenas-Gamboa, A. M. and Maurizio, E., Caprine brucellosis: a historically neglected disease with significant impact on public health. PLoS Negl. Trop. Dis., 2017, 11(8), e0005692; https://doi.org/10.1371/journal.pntd.0005692.

  • Singh, A., Gupta, V. K., Kumar, A., Singh, V. K. and Nayakwadi, S., 16S rRNA and Omp31 gene based molecular characterization of field strains of B. melitensis from aborted foetus of goats in India. Sci. World J., 2013, 2013, 160376; doi:10.1155/2013/160376.

  • Goel, S., Goyal, P., Singh, A., Goel, A. K., Gupta, A., Surana, A. and Bhardwaj, A., Incidence and sero epidemiology of brucellosis from a tertiary care centre of rural Maharashtra. Int. Arch. Integ. Med., 2015, 2(8), 71–77.

  • Agasthya, A. S., Isloor, S. and Krishnamsetty, P., Seroprevalence study of human brucellosis by conventional tests and indigenous indirect enzyme-linked immunosorbent assay. Sci. World J., 2012, 2012, 104239.

  • Renukaradhya, G. J., Isloor, S. and Rajasekhar, M., Epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in India. Veter. Microbiol., 2002, 90, 183–195.

  • Handa, R., Singh, S., Singh, N. and Wali, J. P., Brucellosis in north India: results of a prospective study. J. Commun. Dis., 1998, 30(2), 85–87.

  • Halliday, J. E. B., Allan, K. J., Ekwem, D., Cleaveland, S. and Kazwala, R. R., Endemic zoonoses in the tropics: a public health problem hiding in plain sight. Veter. Rec., 2015, 176, 220–225.

  • Mangalgi, S. S., Sajjan, A. G., Mohite, S. T. and Kakade, S. V., Serological, clinical, and epidemiological profile of human brucellosis in rural India. Indian J. Commun. Med., 2015, 40(3), 163–167.

  • Singh, B. B., Khatkar, M. S., Aulakh, R. S., Gill, J. P. S. and Dhand, N. K., Estimation of the health and economic burden of human brucellosis in India. Prev. Veter. Med., 2018, 154, 148–155.


Abstract Views: 313

PDF Views: 138




  • Gynaecological Disorders Associated with Brucella melitensis in Goat Flocks with Potential Risk of Occupational Zoonoses in Central India

Abstract Views: 313  |  PDF Views: 138

Authors

S. P. Awandkar
College of Veterinary and Animal Sciences, Udgir 413 517, India
S. Kale
Nagpur Veterinary College, Nagpur 440 006, India
C. Sonekar
Nagpur Veterinary College, Nagpur 440 006, India
G. K. Gabhane
Nagpur Veterinary College, Nagpur 440 006, India
S. P. Chaudhari
Nagpur Veterinary College, Nagpur 440 006, India
N. V. Kurkure
Nagpur Veterinary College, Nagpur 440 006, India

Abstract


Brucellosis is an occupational infectious disease affecting both animals and humans. It causes abortions, retention of placenta, stillbirth and infertility in small and large ruminants. It is considered as a disease of economic importance to the society engaged in animal husbandry. The present study was aimed to estimate the prevalence of brucellosis in goats exhibiting abortions, retention of placenta and still births and in occupationally exposed individuals, to type the circulating Brucella species in Central India and risk factors for its spillage to human host. Seroprevalence of 34.72% (RBPT), 33.33% (SAT and S-LPS ELISA) was recorded in goats and 2.38% (RBPT, SAT and S-LPS ELISA) in occupationally exposed individuals. The clinical samples revealed three Brucella melitensis isolates as confirmed by bcsp31, IS711 and AMOS PCR. Early diagnosis, general awareness, restricted animal movement, hygiene, secure handling and disposal of infectious waste is warranted.

Keywords


Brucella melitensis, Goats, Gynaecological Disorders, Occupational Zoonoses, Seroprevalence.

References





DOI: https://doi.org/10.18520/cs%2Fv120%2Fi12%2F1912-1916