The present study was conducted to identify male-specific active plant volatiles from maize against Chilo partellus. The gas chromatography-electroantennogram detector evaluation of volatile extracts collected from maize plant through dynamic headspace collection revealed the presence of six electrophysiologically active volatiles for male, viz. toluene, 2-ethyl-1-hexanol, 4-hydroxy-4-methyl-2-pentanone, 1,4-dichlorobenzene, p-isopropyl benzaldehyde (cuminaldehyde) and p-xylene. Electroantennogram analysis of selective synthetic volatiles against male moths revealed significantly higher response to geranyl acetate, cuminaldehyde, linalool and 2-ethyl-1-hexanol. Electroantennogram studies showed dose-dependent responses of male moths against geranyl acetate, cuminaldehyde, linalool and 2-ethyl-1-hexanol up to 50 mg, above which saturation of antennal response was noticed. These findings will help to study the synergistic activity of identified volatiles with pheromone compounds for developing more effective lure for C. partellus monitoring and mass trapping.
Keywords
Dynamic headspace collection, gas chromatography-electroantennogram detector, maize stem borer, male-specific active volatiles.
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