Aspergillus niger is used for xylanase production on agricultural waste as substrate under broth culture. Rice straw and sugarcane bagasse have been the most potential substrate for xylanase production. Two different mutagens were used: UV radiation for different time durations and 5-bromouracil of different concentrations. Mutants so formed were selected on the basis of morphological and colony characteristics. Selected mutants were checked for their stability, requirement of amino acid and xylanase activity. Tested mutants showed 4-fold increase in xylanase activity from wild type
Keywords
Agricultural waste, mutation, rice straw, sugarcane bagasse, xylanase
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