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Xanthone from Swertia chirata Exerts Chemotherapeutic Potential Against Colon Carcinoma


Affiliations
1 Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India
2 Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme-XM, Beliaghata, Kolkata 700 010, India
3 Department of Genetics and Plant Breeding, University of Calcutta, Kolkata 700 073, India
4 Department of Genetics and Plant Breeding, University of Calcutta, Kolkata 700 073, India, India
5 Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India
 

The present study examines whether 1,5,8-tri-hydroxy-3-methoxy xanthone (TMX) isolated from Swertia chirata could restrict colon cancer cells by downregulating proliferation and inducing apoptosis. The chemotherapeutic activity of TMX was evaluated in several colon cancer and normal cell lines using in vitro assays like MTT assay, cell-cycle analysis, caspase-3 activity assay, annexin V/PI staining, JC10 assay, intracellular reactive oxygen species (ROS) level determination by dicholoro­fluorescein di-acetate (DCFH-DA). The present study revealed that TMX from S. chirata could effectively inhibit proliferation of metastatic colon cancer cell lines. The chemotherapeutic potential of TMX against metastatic colon cancer cell lines was achieved by downregulating several critical regulatory genes enab­ling the suppression of the proliferative potential of colon cancer cells and driving them towards apoptosis in a ROS dependent manner. In addition, TMX showed chemosensitization potential in colon cancer cell lines

Keywords

Apoptosis, Chemosensitization, Colon Cancer, Reactive Oxygen Species, Swertia chirata.
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  • Xanthone from Swertia chirata Exerts Chemotherapeutic Potential Against Colon Carcinoma

Abstract Views: 438  |  PDF Views: 127

Authors

Atish Barua
Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India
Pritha Choudhury
Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India
Niraj Nag
Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme-XM, Beliaghata, Kolkata 700 010, India
Anirban Nath
Department of Genetics and Plant Breeding, University of Calcutta, Kolkata 700 073, India
Sabyasachi Kundagrami
Department of Genetics and Plant Breeding, University of Calcutta, Kolkata 700 073, India, India
Amit Pal
Division of Pathophysiology, National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Scheme-XM, Beliaghata, Kolkata 700 010, India
Chinmay Kumar Panda
Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India
Prosenjit Saha
Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, India

Abstract


The present study examines whether 1,5,8-tri-hydroxy-3-methoxy xanthone (TMX) isolated from Swertia chirata could restrict colon cancer cells by downregulating proliferation and inducing apoptosis. The chemotherapeutic activity of TMX was evaluated in several colon cancer and normal cell lines using in vitro assays like MTT assay, cell-cycle analysis, caspase-3 activity assay, annexin V/PI staining, JC10 assay, intracellular reactive oxygen species (ROS) level determination by dicholoro­fluorescein di-acetate (DCFH-DA). The present study revealed that TMX from S. chirata could effectively inhibit proliferation of metastatic colon cancer cell lines. The chemotherapeutic potential of TMX against metastatic colon cancer cell lines was achieved by downregulating several critical regulatory genes enab­ling the suppression of the proliferative potential of colon cancer cells and driving them towards apoptosis in a ROS dependent manner. In addition, TMX showed chemosensitization potential in colon cancer cell lines

Keywords


Apoptosis, Chemosensitization, Colon Cancer, Reactive Oxygen Species, Swertia chirata.

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DOI: https://doi.org/10.18520/cs%2Fv122%2Fi1%2F47-55