Current Science

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The present study examines whether 1,5,8-tri-hydroxy-3-methoxy xanthone (TMX) isolated from Swertia chirata could restrict colon cancer cells by downregulating proliferation and inducing apoptosis. The chemotherapeutic activity of TMX was evaluated in several colon cancer and normal cell lines using in vitro assays like MTT assay, cell-cycle analysis, caspase-3 activity assay, annexin V/PI staining, JC10 assay, intracellular reactive oxygen species (ROS) level determination by dicholoro­fluorescein di-acetate (DCFH-DA). The present study revealed that TMX from S. chirata could effectively inhibit proliferation of metastatic colon cancer cell lines. The chemotherapeutic potential of TMX against metastatic colon cancer cell lines was achieved by downregulating several critical regulatory genes enab­ling the suppression of the proliferative potential of colon cancer cells and driving them towards apoptosis in a ROS dependent manner. In addition, TMX showed chemosensitization potential in colon cancer cell lines

Keywords

Apoptosis, Chemosensitization, Colon Cancer, Reactive Oxygen Species, Swertia chirata.
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