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Pearl millet is an important staple crop, and biofortifying with grain iron (Fe) and zinc (Zn) is a continuing effort to fight micronutrients malnutrition globally. In the present study, parental polymorphism survey identi­fied 99 polymorphic primers between the contrasting parents out of 275 primers used. Further, in bulk segregant analysis, two simple sequence repeats (SSRs), Xipes0027 and Xpsmp2263 were found to be polymorphic between high and low grain Fe and Zn content parents as well as bulks. The primer Xipes0027 showed the specific band of 214 bp and Xpsmp2263 amplified 238 bp in the high grain Fe and Zn content parents and bulks. Moreover, individual plants of each contrasting bulks were genotyped to validate the result of BSA. Xipes0027 was reported to be present on linkage groups 2 as well as 6 of pearl millet, and Xpsmp2263 was located on linkage group 7 according to the published SSR consensus maps. As a result, these markers have been reported to be putatively linked to elevated Fe and Zn content in grains of pearl millet

Keywords

Biofortification, bulk segregant analysis, grain Fe and Zn content, pearl millet, SSRs.
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