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Alpha-glucosidase inhibition and antioxidant activity of Ensete superbum (Roxb.) Cheesman seeds: GC-MS-based profiling of the active metabolites and molecular docking study


Affiliations
1 Biological Sciences Division, Poornaprajna Institute of Scientific Research, Bidalur Post, Devanahalli, Bangalore Rural 562 110, India; Manipal Academy of Higher Education, Manipal 576 104, India
2 Biological Sciences Division, Poornaprajna Institute of Scientific Research, Bidalur Post, Devanahalli, Bangalore Rural 562 110, India
 

The present study aims to uncover the alpha-gluco­sidase inhibition (AGI) and antioxidant activities of Ensete superbum seed extract. The AGI activity of crude extract (IC50 = 0.17 mg/ml) and silica gel column purified fraction (ESSFR4; IC50 = 0.02 mg/ml) was excellent when compared to acarbose (IC50 = 1720 mg/ml). Also, the free-radical scavenging ability of ESSFR4 was comparable to ascorbic acid. Among the five compounds identified from ESSFR4, pentasiloxane, dodecamethyl and 2,4-hexadienedioic acid, bis(trimethylsilyl) ester, had the best binding affinities against human and yeast AG enzymes respectively. However, ADME/toxicity evaluation of these five compounds revealed that they would require further structural scrutiny and in vivo studies before recommending them as an alternative to the present AGIs to treat type-2 diabetes.

Keywords

Alpha-glucosidase inhibition, antioxidants, diabetes, Ensete superbum, toxicity evaluation.
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  • Alpha-glucosidase inhibition and antioxidant activity of Ensete superbum (Roxb.) Cheesman seeds: GC-MS-based profiling of the active metabolites and molecular docking study

Abstract Views: 395  |  PDF Views: 135

Authors

Kirana P. Mugaranja
Biological Sciences Division, Poornaprajna Institute of Scientific Research, Bidalur Post, Devanahalli, Bangalore Rural 562 110, India; Manipal Academy of Higher Education, Manipal 576 104, India
Ananda Kulal
Biological Sciences Division, Poornaprajna Institute of Scientific Research, Bidalur Post, Devanahalli, Bangalore Rural 562 110, India

Abstract


The present study aims to uncover the alpha-gluco­sidase inhibition (AGI) and antioxidant activities of Ensete superbum seed extract. The AGI activity of crude extract (IC50 = 0.17 mg/ml) and silica gel column purified fraction (ESSFR4; IC50 = 0.02 mg/ml) was excellent when compared to acarbose (IC50 = 1720 mg/ml). Also, the free-radical scavenging ability of ESSFR4 was comparable to ascorbic acid. Among the five compounds identified from ESSFR4, pentasiloxane, dodecamethyl and 2,4-hexadienedioic acid, bis(trimethylsilyl) ester, had the best binding affinities against human and yeast AG enzymes respectively. However, ADME/toxicity evaluation of these five compounds revealed that they would require further structural scrutiny and in vivo studies before recommending them as an alternative to the present AGIs to treat type-2 diabetes.

Keywords


Alpha-glucosidase inhibition, antioxidants, diabetes, Ensete superbum, toxicity evaluation.

References





DOI: https://doi.org/10.18520/cs%2Fv122%2Fi6%2F682-688