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Molecular diversity of Nilaparvata lugens (Stål.) (Hemiptera: Delphacidae) from India based on internal transcribed spacer 1 gene


Affiliations
1 Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
2 ICAR-RCER Farming System Research Centre for Hill and Plateau Region, Ranchi 834 010, India
 

Brown planthopper, Nilaparvata lugens, is the major pest of rice in India and causes significant yield loss. It causes damage by sucking the plant sap leading to a characteristic symptom called ‘hopper burn’. The present study was undertaken to assess the genetic variability of N. lugens populations from different rice ecologies in India, to comprehend and assist in planning proper management strategies. We evaluated the molecular diversity in 17 N. lugens populations based on internal transcribed spacer 1 (ITSI) gene sequences. In all, 53 unique haplotypes were identified and their numbers varied from 1 to 10 in the sampled populations. Genetic diversity indices like nucleotide diversity, haplotype number, haplotype diversity and average number of nucleotide differences revealed low to high levels of genetic diversity among the populations. A highly significant negative relation of Fu’s F and Tajima’s D tests with insignificant sum of square deviation (SSD) values indicated possible recent expansion of N. lugens in different Indian regions with a population expansion time of 3.9 million years. A non-significant correlation in isolation pattern by distance indicated that geographic barriers present in India are inadequate to bring genetic differentiation among N. lugens from different migratory populations. In the present study, the ITSI gene sequence was used to analyse genetic structure among N. lugens in India.

Keywords

Genetic Structure, Haplotypes, Molecular Diversity, Nilaparvata Lugens, Rice
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  • Molecular diversity of Nilaparvata lugens (Stål.) (Hemiptera: Delphacidae) from India based on internal transcribed spacer 1 gene

Abstract Views: 369  |  PDF Views: 147

Authors

Govindharaj Guru-Pirasanna-Pandi
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
Aashish Kumar Anant
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
Jaipal Singh Choudhary
ICAR-RCER Farming System Research Centre for Hill and Plateau Region, Ranchi 834 010, India
Soumya Bharati Babu
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
G. Basana-Gowda
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
M. Annamalai
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
Naveenkumar Patil
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
Totan Adak
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
P. Panneerselvam
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India
Prakash Chandra Rath
Division of Crop Protection, ICAR-National Rice Research Institute, Cuttack 753 006, India

Abstract


Brown planthopper, Nilaparvata lugens, is the major pest of rice in India and causes significant yield loss. It causes damage by sucking the plant sap leading to a characteristic symptom called ‘hopper burn’. The present study was undertaken to assess the genetic variability of N. lugens populations from different rice ecologies in India, to comprehend and assist in planning proper management strategies. We evaluated the molecular diversity in 17 N. lugens populations based on internal transcribed spacer 1 (ITSI) gene sequences. In all, 53 unique haplotypes were identified and their numbers varied from 1 to 10 in the sampled populations. Genetic diversity indices like nucleotide diversity, haplotype number, haplotype diversity and average number of nucleotide differences revealed low to high levels of genetic diversity among the populations. A highly significant negative relation of Fu’s F and Tajima’s D tests with insignificant sum of square deviation (SSD) values indicated possible recent expansion of N. lugens in different Indian regions with a population expansion time of 3.9 million years. A non-significant correlation in isolation pattern by distance indicated that geographic barriers present in India are inadequate to bring genetic differentiation among N. lugens from different migratory populations. In the present study, the ITSI gene sequence was used to analyse genetic structure among N. lugens in India.

Keywords


Genetic Structure, Haplotypes, Molecular Diversity, Nilaparvata Lugens, Rice

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DOI: https://doi.org/10.18520/cs%2Fv122%2Fi12%2F1392-1400