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Genotypic variation in auxin-induced rooting response of detached tomato leaves: an innovative approach for indirect evaluation of yielding ability in tomato (Solanum lycopersicum L.)


Affiliations
1 All India Coordinated Research Project on Vegetable Crops, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
2 Department of Plant Breeding and Genetics, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
3 Department of Vegetable Science, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
 

In this study, the extent of genetic variation in auxin-induced rooting response of detached tomato leaves has been assessed and correlated with yielding ability. Fully expanded, healthy and disease-free compound leaves were excised and treated with 500 ppm aqueous indole butyric acid solution for 45 sec, then washed thoroughly with tap water and kept in beakers filled with distilled water. The leaves were examined randomly for the number of roots induced, length of the longest root, fresh root weight and frequency of rooted leaves to determine variation in auxin response of the genotypes. Auxin-treated detached leaves of 22 tomato genotypes showed wide variation in their root traits. The genotypic coefficient of variation for the number of roots, root length, fresh root weight and frequency of rooted leaf cuttings was 45.43, 11.72, 38.45 and 31.66 respectively. Auxin sensitivity index (ASI) of the genotypes ranged from 0.0 to 3.0. A significant positive correlation (0.647) was observed between ASI and fruit yield. This study reveals that genetic variation in the auxin-induced rooting response of detached tomato leaves could be used as an indicator for screening high-yielding tomato genotypes. This innovative approach is simple, rapid and inexpensive.
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  • Yarwood, C. E., Detached leaf culture. Bot. Rev., 1946, 12, 1–56.

  • Kachru, S., Kumar, P., Sharma, P., Rana, U. and Upadhyay, S. K., Effect of indole butyric acid (IBA), cow urine and growing media on root formation in tomato stem cuttings. Himachal. J. Agric. Res., 2017, 43, 40–43.

  • Pramanik, K. and Mohapatra, P. P., Role of auxin on growth, yield and quality of tomato – a review. Int. J. Curr. Microbiol. Appl. Sci., 2017, 6, 1624–1636.

  • Ashraf, M. I., Sajad, S. and Iqbal, R., Effect of plant growth regulators (IBA and 2,4-D) on the morphology and biochemical characteristics of radish (Raphanus sativus L.). Int. J. Appl. Sci. – Res. Rev., 2018, 5, 6–10.

  • Foolad, M. R., Sullenberger, M. T. and Ashrafi, H., Detached leaflet evaluation of tomato germplasm for late blight resistance and its correspondence to field and green house screening. Americ. Phytopathol. Soc., 2015, 9, 718–722.

  • Meenakshi, S., Mohandas, S. and Mahmood, R., Comparison of detached leaf evaluation in different ages of transgenic tomato expressing antimicrobial peptide gene (ace-AMP1) resistant to early blight disease caused by Alternaria solani. Int. J. Innovat. Res. Sci., Eng. Technol., 2014, 3, 16273–16276.

  • Rubin, E. and Cohen, Y., An improved method for infecting tomato leaves or seedlings with oospores of Phytophthora infestans used to investigate F1 progeny. Americ. Phytopathol. Soc., 2006, 90, 741–749.

  • Kobata, T., Shinonaga, M., Yoshida, H., Tomisaka, K. and Akai, K., Stay green trait assessment using the leaf incubation method to examine the maintenance of assimilation rates under high temperature conditions during the grain filling period in rice. Plant Prod. Sci., 2015, 18, 254–266.

  • Singh, R. K. and Chaudhary, B. D., Biometrical Methods in Quantitative Genetic Analysis, Kalyani Publisher, New Delhi, 1985.

  • Burton, G. W. and de Vane, E. H., Estimating heritability in tall fescue (Festuca arundinacea) from replicated clonal material. Agron. J., 1953, 45, 481–487.

  • Johnson, H. W., Robinson, H. F. and Comstock, R. E., Estimates of genetic and environmental variability in soybeans. Agron. J., 1955, 47, 314–318.

  • Satpathy, P., Das, S., Sahu, G. S., Naresh Babu, P. and Pradhan, B. D., Variation in indigenous auxin response of tomato genotypes and its association with yield. Int. J. Chem. Stud., 2019, 7, 2495–2499.

  • Panse, V. G. and Sukhatme, P. V., Statistical Methods for Agricultural Workers, Publication and Information Division, ICAR, New Delhi, 1985, 4th edn.

  • Wu, L. J., Tian, Z. D. and Zhang, J. H., Functional dissection of auxin response factors in regulating tomato leaf shape development. Front. Plant Sci., 2018, 9, 957.

  • de Jong, M. et al., Solanum lycopersicum auxin response factor 9 regulates cell division activity during early tomato fruit development. J. Exp. Bot., 2015, 66, 3405–3416.

  • de Jong, M., Wolters-Arts, M., Feron, R., Mariani, C. and Vriezen, W. H., The Solanum lycopersicum auxin response factor 7 (SlARF7) regulates auxin signaling during tomato fruit set and development. Plant J., 2009, 57, 160–170.

  • Zhang, X., Yan, F., Tang, Y., Yuan, Y., Deng, W. and Li, Z., Auxin response gene SlARF3 plays multiple roles in tomato development and is involved in the formation of epidermal cells and trichomes. Plant Cell Physiol., 2015, 56, 2110–2124.

  • Bouzroud, S. et al., Auxin response factors (ARFs) are potential mediators of auxin action in tomato response to biotic and abiotic stress (Solanum lycopersicum). PLoS ONE, 2018, 13(2), 1–20.

  • Das, S., Mishra, R. C., Sinha, S. K. and Pattanaik, M. C., Variation in streptomycin-induced bleaching and dark induced senescence of rice (Oryza sativa) genotypes and their relationship with yield and adaptability. J. Plant Breed. Crop Sci., 2010, 2, 139–147.

  • Sinha, S. K., Das, S., Mishra, R. C. and Singh, B., Novel approaches and techniques of cost saving potential in crop improvement. J. Appl. Sci., 2010, 10, 1885–1893.

  • Das, S., Mishra, R. C. and Sinha, S. K., Variation in seedling growth inhibition due to maleic hydrazide treatment of rice (O. sativa) and ragi (E. coracana) genotypes and its relationship with yield and adaptability. J. Crop Sci. Biotechnol., 2008, 11, 215–222.

  • Singh, B. R., Das, S. and Sinha, S. K., Laboratory evaluation of adaptation through seedling response to digitonin and indole-butyric acid in mungbean. Legume Res., 2011, 34, 278–282.


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  • Genotypic variation in auxin-induced rooting response of detached tomato leaves: an innovative approach for indirect evaluation of yielding ability in tomato (Solanum lycopersicum L.)

Abstract Views: 161  |  PDF Views: 85

Authors

S. Das
All India Coordinated Research Project on Vegetable Crops, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
P. Satpathy
Department of Plant Breeding and Genetics, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
S. K. Dash
All India Coordinated Research Project on Vegetable Crops, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
A. Mohanty
All India Coordinated Research Project on Vegetable Crops, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
S. Sarkar
All India Coordinated Research Project on Vegetable Crops, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
B. Pradhan
Department of Plant Breeding and Genetics, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
G. S. Sahu
Department of Vegetable Science, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
P. K. Tripathy
Department of Vegetable Science, Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India

Abstract


In this study, the extent of genetic variation in auxin-induced rooting response of detached tomato leaves has been assessed and correlated with yielding ability. Fully expanded, healthy and disease-free compound leaves were excised and treated with 500 ppm aqueous indole butyric acid solution for 45 sec, then washed thoroughly with tap water and kept in beakers filled with distilled water. The leaves were examined randomly for the number of roots induced, length of the longest root, fresh root weight and frequency of rooted leaves to determine variation in auxin response of the genotypes. Auxin-treated detached leaves of 22 tomato genotypes showed wide variation in their root traits. The genotypic coefficient of variation for the number of roots, root length, fresh root weight and frequency of rooted leaf cuttings was 45.43, 11.72, 38.45 and 31.66 respectively. Auxin sensitivity index (ASI) of the genotypes ranged from 0.0 to 3.0. A significant positive correlation (0.647) was observed between ASI and fruit yield. This study reveals that genetic variation in the auxin-induced rooting response of detached tomato leaves could be used as an indicator for screening high-yielding tomato genotypes. This innovative approach is simple, rapid and inexpensive.

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DOI: https://doi.org/10.18520/cs%2Fv123%2Fi4%2F568-573