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In silico evidence for extensive Ser/Thr phosphorylation of Mycobacterium tuberculosis two-component signalling systems


Affiliations
1 Department of Biochemistry, Sri Venkateswara College, University of Delhi, New Delhi 110 021, India, India
 

Mycobacterium tuberculosis has the innate ability to adapt and survive the intracellular environments during infection. Two-component signalling (TCS) systems and serine (Ser)/threonine (Thr) protein kinases facilitate metabolic and growth adaptation by directing transcri­p­tomic reprogramming in response to environmental stimuli. Presently, little is known about the post-transla­tional regulation of TCS proteins through O-phospho­rylation. Using the NetPhosBac 1.0 in silico tool, we screened components of M. tuberculosis TCS systems for potential Ser/Thr phosphosites. We report extensive Ser/Thr phosphorylation of sensor kinases and response regulator proteins, suggesting that it might be a distinct mechanism enabling the co-regulation of pathways impac­ting adaptive changes in mycobacterial growth and meta­bolism

Keywords

Mycobacterium tuberculosis, post-transla-tional modification, serine/threonine protein kinase, two -component systems response regulators.
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  • In silico evidence for extensive Ser/Thr phosphorylation of Mycobacterium tuberculosis two-component signalling systems

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Authors

Abhishek Garg
Department of Biochemistry, Sri Venkateswara College, University of Delhi, New Delhi 110 021, India, India
Nimisha Khurana
Department of Biochemistry, Sri Venkateswara College, University of Delhi, New Delhi 110 021, India, India
Ananya Chugh
Department of Biochemistry, Sri Venkateswara College, University of Delhi, New Delhi 110 021, India, India
Kangna Verma
Department of Biochemistry, Sri Venkateswara College, University of Delhi, New Delhi 110 021, India, India
Vandana Malhotra
Department of Biochemistry, Sri Venkateswara College, University of Delhi, New Delhi 110 021, India, India

Abstract


Mycobacterium tuberculosis has the innate ability to adapt and survive the intracellular environments during infection. Two-component signalling (TCS) systems and serine (Ser)/threonine (Thr) protein kinases facilitate metabolic and growth adaptation by directing transcri­p­tomic reprogramming in response to environmental stimuli. Presently, little is known about the post-transla­tional regulation of TCS proteins through O-phospho­rylation. Using the NetPhosBac 1.0 in silico tool, we screened components of M. tuberculosis TCS systems for potential Ser/Thr phosphosites. We report extensive Ser/Thr phosphorylation of sensor kinases and response regulator proteins, suggesting that it might be a distinct mechanism enabling the co-regulation of pathways impac­ting adaptive changes in mycobacterial growth and meta­bolism

Keywords


Mycobacterium tuberculosis, post-transla-tional modification, serine/threonine protein kinase, two -component systems response regulators.

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DOI: https://doi.org/10.18520/cs%2Fv123%2Fi9%2F1164-1169