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DNA Barcoding of Rotifers along the Cochin Backwaters, Southwest Coast of India


Affiliations
1 Central Marine Fisheries Research Institute, Ernakulam North, Kochi 682 018, India
 

This study examines the DNA barcoding of rotifers from the Cochin backwaters, Kerala, India, with specimens obtained from the pure culture, using primers LCO1490/ HCO2198 and a recently reported primer 30F/885R which was designed especially for the rotifers. Partial sequences of the mitochondrial COI gene from five species belonging to three genera (Brachionus, Keratella and Asplanchna) and two families (Brachionidae and Asplanchnidae) of the order Ploimida were generated. All the obtained nucleotide sequences were submitted to NCBI (accession numbers: OL477582–OL477584, OL477586 and OL757796).

Keywords

Backwaters, DNA Barcoding, Partial Sequences, Pure Culture, Rotifer.
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  • DNA Barcoding of Rotifers along the Cochin Backwaters, Southwest Coast of India

Abstract Views: 197  |  PDF Views: 83

Authors

Asha Ravi
Central Marine Fisheries Research Institute, Ernakulam North, Kochi 682 018, India
Sandhya Sukumaran
Central Marine Fisheries Research Institute, Ernakulam North, Kochi 682 018, India
Molly Varghese
Central Marine Fisheries Research Institute, Ernakulam North, Kochi 682 018, India
Neenu Raj
Central Marine Fisheries Research Institute, Ernakulam North, Kochi 682 018, India

Abstract


This study examines the DNA barcoding of rotifers from the Cochin backwaters, Kerala, India, with specimens obtained from the pure culture, using primers LCO1490/ HCO2198 and a recently reported primer 30F/885R which was designed especially for the rotifers. Partial sequences of the mitochondrial COI gene from five species belonging to three genera (Brachionus, Keratella and Asplanchna) and two families (Brachionidae and Asplanchnidae) of the order Ploimida were generated. All the obtained nucleotide sequences were submitted to NCBI (accession numbers: OL477582–OL477584, OL477586 and OL757796).

Keywords


Backwaters, DNA Barcoding, Partial Sequences, Pure Culture, Rotifer.

References





DOI: https://doi.org/10.18520/cs%2Fv124%2Fi3%2F327-332