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Genetic Analysis of Brown Planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) Based on Microsatellite Markers


Affiliations
1 ICAR-National Rice Research Institute, Cuttack 753 006, India
2 Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
 

Brown planthopper, Nilaparvata lugens (Stål) is one of the most destructive pests of rice in Southeast Asia. It expresses a differential reaction to resistant rice cultivars and various insecticide groups in different geographic locations. Therefore, genetic diversity among N. lugens populations must be understood for their effective management. Hence, in the present study, the genetic structure and diversity of 22 N. lugens populations collected from 22 hotspot regions of India were analysed using with genomic simple sequence repeat (SSR) markers. Results revealed that the mean genetic diversity was 0.399 and polymorphic information content was 0.337 in the 30 selected SSR markers. Further, molecular variance revealed only a 2% variation among the populations and 98% within a population. In cluster and population structure analysis, all 22 populations were sub-grouped into three groups. Interestingly, the North and West Indian populations showed high genetic similarity and assembled into one cluster in cluster analysis. The East and South Indian populations were evenly segregated into the remaining two clusters. Similarly, the North and West Indian populations shared the same compartment in principal coordinate analysis. This variation might be associated with the N. lugens migration due to wind movement of the southwest monsoon in two branches, viz. Arabian Sea branch and Bay of Bengal branch. The present study provides molecular evidence for genetic variation among different populations of N. lugens in India. The information could be helpful to devise an efficient management strategy against this pest in different rice ecosystems.

Keywords

Brown Planthopper, Genetic Diversity, Micro-Satellite Markers, Monsoon, Rice.
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  • Pathak, H., Tripathi, R., Jambhulkar, N. N., Bisen, J. P. and Panda, B. B., Eco-regional rice farming for enhancing productivity, profitability and sustainability, NRRI Research Bulletin No. 22, ICAR-National Rice Research Institute, Cuttack, 2020, p. 28.

  • Hunter, M. C., Smith, R. G., Schipanski, M. E., Atwood, L. W. and Mortensen, D. A., Agriculture in 2050: recalibrating targets for sustainable intensification. BioScience, 2017, 67(4), 386–391; https://doi.org/10.1093/biosci/bix010.

  • Mondal, D. et al., Yield loss assessment of rice (Oryza sativa L.) due to different biotic stresses under system of rice intensification (SRI). J. Entomol. Zool. Stud., 2017, 5(4), 1974–1980.

  • Pandi, G. G. P., Chandar, S., Pal, M. and Soumia, P. S., Impact of elevated CO2 on Oryza sativa phenology and brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) population. Curr. Sci., 2018, 114(8), 1767–1777.

  • Jena, M. et al., Paradigm shift of insect pests in rice ecosystem and their management strategy. Oryza, 2018, 55, 82–89.

  • Anant, A. et al., Evaluation of brown plant hopper Nilaparvata lugens (Stal) resistance. Indian J. Entomol., 2021, 83(2), 223–225.

  • Horgan, F. G., Penalver, C. A., Arida, A., Ferrater, J. B. and Bernal, C. C., Adaptation by the brown planthopper to resistant rice: a test of female-derived virulence and the role of yeast-like symbionts. Insects, 2021, 12, 908; https://doi.org/10.3390/insects12100908.

  • Hu, G. et al., Outbreaks of the brown planthopper Nilaparvata lugens (Stål) in the Yangtze River Delta: immigration or local reproduction? PLoS ONE, 2014, 9(2), e88973; https://doi.org/10.1371/journal.pone.0088973.

  • Muduli, L., Pradhan, S. K., Mishra, A., Bastia, D. N., Samal, K. C., Agrawal, P. K. and Dash, M., Understanding brown planthopper resistance in rice: genetics, biochemical and molecular breeding approaches. Rice Sci., 2021, 28(6), 532–546; https://doi.org/10.1016/j.rsci.2021.05.013.

  • Babu, S. B. et al., Genomic analysis and finding of candidate genes for Nilaparvata lugens (Stål) resistance in Indian pigmented and other indigenous rice genotypes. Crop Prot., 2022, 156, 105959; https://doi.org/10.1016/j.cropro.2022.105959.

  • Pandi, G. G. P. et al., Molecular diversity of Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) from India based on internal transcribed spacer 1 (ITS1) gene. Curr. Sci., 2022, 122(12), 1392–1400.

  • Anant, A. K. et al., Genetic dissection and identification of candidate genes for brown planthopper, Nilaparvata lugens (Delphacidae: Hemiptera) resistance in ‘farmers’ varieties of rice in Odisha. Crop Prot., 2021, 144, 105600.

  • Mohan, U., Lakshmi, V. J., Sharma, S., Katti, G. R., Chirutkar, P. M. and Krishnaiah, N. V., Monitoring of insecticide resistance in rice brown planthopper Nilaparvata lugens (Stål) in Nalgonda District of Telangana state, India. Ann. Plant Prot. Sci., 2019, 27(2), 172–176.

  • Deosi, K. K. and Suri, K. S., Status of insecticide resistance in rice brown planthopper (Nilaparvata lugens) in Punjab. Indian J. Agric. Sci., 2022, 92(2), 203–207; https://doi.org/10.56093/ijasv92i2.122216.

  • Garrood, W. T., Zimmer, C. T., Gorman, K. J., Nauen, R., Bass, C. and Davies, T. G., Field-evolved resistance to imidacloprid and ethiprole in populations of brown planthopper Nilaparvata lugens collected from across South and East Asia. Pest Manage. Sci., 2016, 72, 140–149.

  • Wu, S. F. et al., The evolution of insecticide resistance in the brown planthopper (Nilaparvata lugens Stål) of China in the period 2012–2016. Sci. Rep., 2018, 8, 4586; https://doi.org/10.1038/s41598-018-22906-5.

  • Basanth, Y. S., Sannaveerappanavar, V. T. and Gowda, D. K. S., Susceptibility of different populations of Nilaparvata lugens from major rice growing areas of Karnataka, India to different groups of insecticides. Rice Sci., 2013, 20(5), 371–378.

  • Ding, Z., Wen, Y., Yang, B., Zhang, Y., Liu, S., Liu, Z. and Han, Z., Biochemical mechanisms of imidacloprid resistance in Nilaparvatalugens: over-expression of cytochrome P450 CYP6AY1. Insect Biochem. Mol. Biol., 2013, 43, 1021–1027.

  • Vieira, M. L., Santini, L., Diniz, A. L. and Munhoz, C., Microsatellite markers: what they mean and why they are so useful. Genet. Mol. Biol., 2016, 39(3), 312–328; https://doi.org/10.1590/1678-4685-GMB-2016-0027.

  • Benali, S., Mohamed, B., Eddine, H. J. and Neema, C., Advances of molecular markers application in plant pathology research. Eur. J. Sci. Res., 2011, 50, 110–123.

  • Ince, A. G., Mehmet, K. and Safinaz, E., New microsatellite and CAPS-microsatellite markers for clarifying taxonomic and phylo-genetic relationships within Origanum L., Mol. Breed., 2014, 34, 643–654.

  • Hashem, M. H. et al., Genetic divergence and phylogenetic relationship of the rabbitfish Siganusrivulatus inferred from microsatellite and mitochondrial markers. J. King Saud Univ. Sci., 2022, 34(4), 101943; https://doi.org/10.1016/j.jksus.2022.101943.

  • Liu, K. and Muse, S. V., Power marker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005, 21, 2128–2129.

  • Peakall, R. and Smouse, P. E., GenAlEx6.5: genetic analysis in Excel population genetic software for teaching and research: an update. Bioinformatics, 2012, 28, 2537–2539.

  • Mantel, N., The detection of disease clustering and a generalized regression approach. Cancer Res., 1967, 27, 209–220.

  • Earl, D. A., STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv. Genet. Resour., 2012, 4, 359–361.

  • Evanno, G., Regnaut, S. and Goudet, J., Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol., 2005, 14, 2611–2620.

  • Jena, K. K. and Kim, S., Current status of brown planthopper (N. lugens) resistant and genetics. Rice, 2010, 3, 161–171.

  • AICRIP, All-India Coordinated Rice Improvement Programme progress report (Entomology Plant Pathology). ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, 2018, vol. 2, pp. 2–3.

  • Duan, X. L., Peng, X., Qiao, X. F. and Chen, M. H., Life cycle and population genetics of bird cherry-oat aphids Rhopalosiphum padi (L.) (Hemiptera: Aphididae) in China: an important pest on wheat crops. J. Pest Sci., 2017, 90, 103–116.

  • Jing, S., Liu, B., Peng, L., Peng, X., Zhu, L., Fu, Q. and He, G., Development and use of EST-SSR markers for assessing genetic diversity in the brown planthopper (Nilaparvata lugens Stål). Bull. Entomol. Res., 2012, 102, 113–122.

  • Serrote, C. M. L., Reiniger, L. R. S., Silva, K. B., Rabaiolli, S. M. D. S. and Stefanel, C. M., Determining the polymorphism information content of a molecular marker. Gene, 2020, 726, 144175; https://doi.org/10.1016/j.gene.2019.144175.

  • Srinivasa, N., Chander, S., Twinkle, S. and Chandel, R. K., Genetic homogeneity in brown planthopper, Nilaparvata lugens (Stål) as revealed from mitochondrial cytochrome oxidase I. Curr. Sci., 2020, 119(6), 1045–1050.

  • Tyagi, S., Narayana, S., Singh, R. N., Srivastava, C. P., Twinkle, S., Das, S. K. and Jeer, M., Migratory behaviour of brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), in India as inferred from genetic diversity and reverse trajectory analysis. 3 Bio-tech., 2022, 12, 266; https://doi.org/10.1007/s13205-022-03337-6.

  • Llewellyn, K. S., Loxdale, H. D., Harrington, R., Brookes, C. P., Clark, S. J. and Sunnucks, P., Migration and genetic structure of the grain aphid (Sitobion avenae) in Britain related to climate and clonal fluctuation as revealed using microsatellites. Mol. Ecol., 2003, 12, 21–34.

  • Liu, J. N., Guia, F. R. and Lib, Z. Y., Genetic diversity of the planthopper, Sogatella furcifera in the Greater Mekong Subregion detected by inter-simple sequence repeats (ISSR) markers. J. Insect Sci., 2010, 10(52), 1–19.

  • Yang, S. J., Bao, Y. X., Chen, C., Lu, M. H., Liu, W. C. and Hong, S. J., Effect of the Asian monsoon on the northward migration of the brown planthopper to northern South China. Ecosphere, 2022, 13(10), e4217.

  • Li, M. M., Li, B. L., Jiang, S. X., Zhao, Y. W., Xu, X. L. and Wu, J. X., Microsatellite-based analysis of genetic structure and gene flow of Mythimna separate (Walker) (Lepidoptera: Noctuidae) in China. Ecol. Evol., 2019, 9(23), 13426–13437; doi:10.1002/ece3.5799. PMID: 31871655; PMCID: PMC6912921.

  • Otuka, A., Dudhia, J., Watanabe, T. and Furuno, A., A new trajectory analysis method for migratory planthoppers, Sogatella furcifera (Horváth) (Homoptera: Delphacidae) and Nilaparvata lugens (Stål), using an advanced weather forecast model. Agric. Entomol., 2005, 7(1), 1–9.

  • Nam, H. Y., Park, Y. and Lee, J. H., Population genetic structure of Aphis gossypii glover (Hemiptera: Aphididae) in Korea. Insects, 2019, 10(10), 319; doi:10.3390/insects10100319. PMID: 31561591; PMCID: PMC6835795.

  • Winnie, R. M., Rafudin, R., Widiarta, I. N. and Rauf, A., The genetic structure of Nilaparvata lugens (Stal) in Java populations. Hayati. J. Biosci., 2020, 27(4), 333–334.

  • Babu, S. B. et al., Genetic analysis of brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) based on DNA markers. Research Square preprint, posted on 30 January 2023; https://doi.org/10.21203/rs.3.rs-2508969/v1.


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  • Genetic Analysis of Brown Planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae) Based on Microsatellite Markers

Abstract Views: 167  |  PDF Views: 102

Authors

Soumya Bharati Babu
ICAR-National Rice Research Institute, Cuttack 753 006, India
Govindharaj Guru-Pirasanna-Pandi
ICAR-National Rice Research Institute, Cuttack 753 006, India
C. Parameswaran
ICAR-National Rice Research Institute, Cuttack 753 006, India
Jayaraj Padhi
Odisha University of Agriculture and Technology, Bhubaneswar 751 003, India
G. Basana-Gowda
ICAR-National Rice Research Institute, Cuttack 753 006, India
M. Annamalai
ICAR-National Rice Research Institute, Cuttack 753 006, India
Naveenkumar Patil
ICAR-National Rice Research Institute, Cuttack 753 006, India
Chanchala Meher
ICAR-National Rice Research Institute, Cuttack 753 006, India
S. Sabarinathan
ICAR-National Rice Research Institute, Cuttack 753 006, India
Prakash Chandra Rath
ICAR-National Rice Research Institute, Cuttack 753 006, India

Abstract


Brown planthopper, Nilaparvata lugens (Stål) is one of the most destructive pests of rice in Southeast Asia. It expresses a differential reaction to resistant rice cultivars and various insecticide groups in different geographic locations. Therefore, genetic diversity among N. lugens populations must be understood for their effective management. Hence, in the present study, the genetic structure and diversity of 22 N. lugens populations collected from 22 hotspot regions of India were analysed using with genomic simple sequence repeat (SSR) markers. Results revealed that the mean genetic diversity was 0.399 and polymorphic information content was 0.337 in the 30 selected SSR markers. Further, molecular variance revealed only a 2% variation among the populations and 98% within a population. In cluster and population structure analysis, all 22 populations were sub-grouped into three groups. Interestingly, the North and West Indian populations showed high genetic similarity and assembled into one cluster in cluster analysis. The East and South Indian populations were evenly segregated into the remaining two clusters. Similarly, the North and West Indian populations shared the same compartment in principal coordinate analysis. This variation might be associated with the N. lugens migration due to wind movement of the southwest monsoon in two branches, viz. Arabian Sea branch and Bay of Bengal branch. The present study provides molecular evidence for genetic variation among different populations of N. lugens in India. The information could be helpful to devise an efficient management strategy against this pest in different rice ecosystems.

Keywords


Brown Planthopper, Genetic Diversity, Micro-Satellite Markers, Monsoon, Rice.

References





DOI: https://doi.org/10.18520/cs%2Fv125%2Fi7%2F777-783