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Effect of 1,25-Dihydroxy Vitamin D3 on Toll-like Receptor Gene Expression in Neutrophils of Pulmonary Tuberculosis


Affiliations
1 Department of Immunology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Indian Council of Medical Research, 1, Mayor Sathyamoorthy Road, Chennai 600 031, India
2 Institute for Thoracic Medicine, Chetput, Chennai 600 031, India
 

We studied the effect of 1,25-dihydroxy vitamin D3 [1,25(OH)2D,3] on toll-like receptor (TLR) gene expression in neutrophils cultured with Mycobacterium tuberculosis (Mtb) with or without 1,25(OH)2D3 in pulmonary tuberculosis patients and healthy controls. 1,25(OH)2D3 significantly increased the expression of TLR2, TLR9 and TIRAP genes compared to control cells in both the study groups (P < 0.05). In healthy controls, a significantly upregulated expression of TIRAP was observed in Mtb infected cells cultured with 1,25(OH)2D3 compared to Mtb infected cells alone (P < 0.05). In conclusion, 1,25(OH)2D3 upregulated the expression of TLR and TIRAP genes, which could lead to the activation of TLR signalling pathways and augment the innate immunity to tuberculosis.

Keywords

Gene Expression, Neutrophils, Tuberculosis, Toll-like Receptors.
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  • Effect of 1,25-Dihydroxy Vitamin D3 on Toll-like Receptor Gene Expression in Neutrophils of Pulmonary Tuberculosis

Abstract Views: 385  |  PDF Views: 141

Authors

M. Harishankar
Department of Immunology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Indian Council of Medical Research, 1, Mayor Sathyamoorthy Road, Chennai 600 031, India
K. Afsal
Department of Immunology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Indian Council of Medical Research, 1, Mayor Sathyamoorthy Road, Chennai 600 031, India
N. Meenakshi
Institute for Thoracic Medicine, Chetput, Chennai 600 031, India
P. Selvaraj
Department of Immunology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Indian Council of Medical Research, 1, Mayor Sathyamoorthy Road, Chennai 600 031, India

Abstract


We studied the effect of 1,25-dihydroxy vitamin D3 [1,25(OH)2D,3] on toll-like receptor (TLR) gene expression in neutrophils cultured with Mycobacterium tuberculosis (Mtb) with or without 1,25(OH)2D3 in pulmonary tuberculosis patients and healthy controls. 1,25(OH)2D3 significantly increased the expression of TLR2, TLR9 and TIRAP genes compared to control cells in both the study groups (P < 0.05). In healthy controls, a significantly upregulated expression of TIRAP was observed in Mtb infected cells cultured with 1,25(OH)2D3 compared to Mtb infected cells alone (P < 0.05). In conclusion, 1,25(OH)2D3 upregulated the expression of TLR and TIRAP genes, which could lead to the activation of TLR signalling pathways and augment the innate immunity to tuberculosis.

Keywords


Gene Expression, Neutrophils, Tuberculosis, Toll-like Receptors.



DOI: https://doi.org/10.18520/cs%2Fv107%2Fi4%2F642-645