Reassembly of DNA Fragments Using Row Access Method

Sequence assembly is a difficult problem whose importance is continuously growing even recently the cost of sequencing has dramatically decreased. The computer simulation for the evolution of sequence assembly of linear sequences have demonstrated the importance of recombination of blocks of sequences. Repeated cycles of point mutagenesis, recombination and selection should allow in "in vitro" molecular evolution of complex sequences, such as proteins. A method for the reassembly of genes from their random DNA fragments, resulting in "in vitro" recombination is reported by Stemmer and there are some laboratory techniques also available that results in reliable sequencing of approximately 500 nucleotides (500-mers). If we have lots and lots of 500-mers than the original genome (from which these 500-mers may be obtained) sequence will be assembled. All such methods are based on image fragmentation reassembly. In this paper we are proposing a technique that is based on tabular form. In the proposed technique we develop a table, which has four rows and two columns and contained information about the base character and position of the base character in the DNA sequence.