Himachal Journal of Agricultural Research

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In-Vitro Assays on Mangifera indica and Embelia ribes against Ascaridia galli of poultry


Affiliations
1 Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
 

The anthelmintic effect of indigenous medicinal plants namely Embelia ribes (seed) and Mangifera indica (bark) was evaluated against the exogenous stages of the poultry nematode Ascaridia galli. In vitro egg embryonation assay showed that the methanolic extract of Embelia ribes showed better inhibitory effect (61.23%) on the embryonation of eggs of Ascaridia galli than its aqueous extract (58.20%). Both M. indica and E. ribes had low inhibitory effect on the activity of second larval stage (L2) inside egg shell. The in vitro assay for larvicidal activity on the deshelled larvae of A. galli revealed that the aqueous extract of Embelia ribes at a concentration of 20 mg/ml exhibited moderate inhibitory effect of 77.66 ± 1.85 %. Adult motility assay revealed that the methanolic extract of E. ribes had a significant anthelmintic efficacy of 88.33%. The extracts from M. indica were found to have very low to moderate anthelmintic properties against various developmental stages of A. galli.

Keywords

Ascaridia galli, Embelia ribes, Mangifera indica, poultry.
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  • In-Vitro Assays on Mangifera indica and Embelia ribes against Ascaridia galli of poultry

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Authors

Divya Sen
Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Rajeev Kumar Agnihotri
Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Devina Sharma
Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Aman Dev Moudgil
Department of Veterinary Parasitology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India

Abstract


The anthelmintic effect of indigenous medicinal plants namely Embelia ribes (seed) and Mangifera indica (bark) was evaluated against the exogenous stages of the poultry nematode Ascaridia galli. In vitro egg embryonation assay showed that the methanolic extract of Embelia ribes showed better inhibitory effect (61.23%) on the embryonation of eggs of Ascaridia galli than its aqueous extract (58.20%). Both M. indica and E. ribes had low inhibitory effect on the activity of second larval stage (L2) inside egg shell. The in vitro assay for larvicidal activity on the deshelled larvae of A. galli revealed that the aqueous extract of Embelia ribes at a concentration of 20 mg/ml exhibited moderate inhibitory effect of 77.66 ± 1.85 %. Adult motility assay revealed that the methanolic extract of E. ribes had a significant anthelmintic efficacy of 88.33%. The extracts from M. indica were found to have very low to moderate anthelmintic properties against various developmental stages of A. galli.

Keywords


Ascaridia galli, Embelia ribes, Mangifera indica, poultry.

References