The present investigation reports effects of disinfectants on culture contamination, seed germination and seedling growth in eggplant. Mercuric chloride hampers seed germination when seeds are treated with 0.1% solution above 2 min duration and lesser durations are not helpful in controlling in vitro contamination. The highest seed germination (89.97%) was recorded with 50% commercial bleach for 20 min in the genotype BL-3, followed by BR-16 (88.53%), BR-14 (86.19%), BL-5 (86.16%) and BSR-23 (85.57%). However, least number of seeds germinated in BR-18 (10.94%), BSR-25 (13.27%) and BL-7 (18.62%) when disinfected with 75% commercial bleach for 25min. Overall results revealed that 50% commercial bleach concentration (73.76%) was better than 75% (36.85%), and 20 min duration (60.82%) was better than 25 min (49.80%) for seed disinfection. Among the varieties, BL-3 was at the top (68.85%) and BR-18 at the lowest (34.16%) edge for per cent seed germination. Seedling growth (cm) with use of commercial bleach was quite satisfactory compared to disinfection with HgCl2, where, 50% commercial bleach favored a good stand of plant lets even after 10 days, showing a healthy ischolar_main (3cm), hypocotyl (5cm) and cotyledons (1.5cm).
Keywords
Seed Germination, Seedling Growth, Culture Contamination, Bleach, Mercuric Chloride, Sodium Hypochlorite.
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