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Evaluation of Dengue Non Structural Antigen ELISA for the Diagnosis of Dengue Virus Infection in the Acute Phase of Illness


Affiliations
1 Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Dr A L Nair Road, Mumbai Central, Opp. Maratha Mandir, Mumbai-400 008, India
2 Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Mumbai, India
     

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Introduction: Dengue viruses are a major public health problem in tropical and subtropical areas, being the cause of one of the most important mosquito-borne viral diseases. Outbreaks have also been reported from Mumbai and other parts of Maharashtra.

Aims and Objectives: (1) Detection and evaluation of Non-Structural Protein (NS1 Ag) in the sera of suspected cases of dengue virus infection. (2) Evaluation of Rapid Immunochromatographic test and ELISA for detection of IgM/IgG antibodies.

Material and Methods: An open labeled prospective study was carried out using 150 patient's sera and 30 paired sera. The serum samples were subjected to Rapid Immunochromatographic test, ELISA and PCR tests.

Results: The sensitivity of Rapid test as compared to ELISA was 87.5% for IgM and 66.6% for IgG. Specificity for ELISA and Rapid test was 96.3% and 92.6% respectively. Sensitivity of NS1 Ag as compared to Dengue PCR was 86.66%.

Conclusion: Dengue NS1 Ag ELISA with its ability to specifically diagnose dengue in early stage of the disease with high sensitivity can prove a boon in the early treatment and management of dengue patients.


Keywords

Dengue, IgM ELISA, NS1 Ag ELISA.
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  • Evaluation of Dengue Non Structural Antigen ELISA for the Diagnosis of Dengue Virus Infection in the Acute Phase of Illness

Abstract Views: 198  |  PDF Views: 0

Authors

J. Shastri
Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Dr A L Nair Road, Mumbai Central, Opp. Maratha Mandir, Mumbai-400 008, India
S. Sawant
Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Mumbai, India
S. Agrawal
Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Mumbai, India

Abstract


Introduction: Dengue viruses are a major public health problem in tropical and subtropical areas, being the cause of one of the most important mosquito-borne viral diseases. Outbreaks have also been reported from Mumbai and other parts of Maharashtra.

Aims and Objectives: (1) Detection and evaluation of Non-Structural Protein (NS1 Ag) in the sera of suspected cases of dengue virus infection. (2) Evaluation of Rapid Immunochromatographic test and ELISA for detection of IgM/IgG antibodies.

Material and Methods: An open labeled prospective study was carried out using 150 patient's sera and 30 paired sera. The serum samples were subjected to Rapid Immunochromatographic test, ELISA and PCR tests.

Results: The sensitivity of Rapid test as compared to ELISA was 87.5% for IgM and 66.6% for IgG. Specificity for ELISA and Rapid test was 96.3% and 92.6% respectively. Sensitivity of NS1 Ag as compared to Dengue PCR was 86.66%.

Conclusion: Dengue NS1 Ag ELISA with its ability to specifically diagnose dengue in early stage of the disease with high sensitivity can prove a boon in the early treatment and management of dengue patients.


Keywords


Dengue, IgM ELISA, NS1 Ag ELISA.