The Indian Practitioner

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of Dengue Non Structural Antigen ELISA for the Diagnosis of Dengue Virus Infection in the Acute Phase of Illness


Affiliations
1 Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Dr A L Nair Road, Mumbai Central, Opp. Maratha Mandir, Mumbai-400 008, India
2 Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Mumbai, India
     

   Subscribe/Renew Journal


Introduction: Dengue viruses are a major public health problem in tropical and subtropical areas, being the cause of one of the most important mosquito-borne viral diseases. Outbreaks have also been reported from Mumbai and other parts of Maharashtra.

Aims and Objectives: (1) Detection and evaluation of Non-Structural Protein (NS1 Ag) in the sera of suspected cases of dengue virus infection. (2) Evaluation of Rapid Immunochromatographic test and ELISA for detection of IgM/IgG antibodies.

Material and Methods: An open labeled prospective study was carried out using 150 patient's sera and 30 paired sera. The serum samples were subjected to Rapid Immunochromatographic test, ELISA and PCR tests.

Results: The sensitivity of Rapid test as compared to ELISA was 87.5% for IgM and 66.6% for IgG. Specificity for ELISA and Rapid test was 96.3% and 92.6% respectively. Sensitivity of NS1 Ag as compared to Dengue PCR was 86.66%.

Conclusion: Dengue NS1 Ag ELISA with its ability to specifically diagnose dengue in early stage of the disease with high sensitivity can prove a boon in the early treatment and management of dengue patients.


Keywords

Dengue, IgM ELISA, NS1 Ag ELISA.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 251

PDF Views: 0




  • Evaluation of Dengue Non Structural Antigen ELISA for the Diagnosis of Dengue Virus Infection in the Acute Phase of Illness

Abstract Views: 251  |  PDF Views: 0

Authors

J. Shastri
Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Dr A L Nair Road, Mumbai Central, Opp. Maratha Mandir, Mumbai-400 008, India
S. Sawant
Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Mumbai, India
S. Agrawal
Department of Microbiology, T.N. Medical College & B.Y.L. Nair Charitable Hospital, Mumbai, India

Abstract


Introduction: Dengue viruses are a major public health problem in tropical and subtropical areas, being the cause of one of the most important mosquito-borne viral diseases. Outbreaks have also been reported from Mumbai and other parts of Maharashtra.

Aims and Objectives: (1) Detection and evaluation of Non-Structural Protein (NS1 Ag) in the sera of suspected cases of dengue virus infection. (2) Evaluation of Rapid Immunochromatographic test and ELISA for detection of IgM/IgG antibodies.

Material and Methods: An open labeled prospective study was carried out using 150 patient's sera and 30 paired sera. The serum samples were subjected to Rapid Immunochromatographic test, ELISA and PCR tests.

Results: The sensitivity of Rapid test as compared to ELISA was 87.5% for IgM and 66.6% for IgG. Specificity for ELISA and Rapid test was 96.3% and 92.6% respectively. Sensitivity of NS1 Ag as compared to Dengue PCR was 86.66%.

Conclusion: Dengue NS1 Ag ELISA with its ability to specifically diagnose dengue in early stage of the disease with high sensitivity can prove a boon in the early treatment and management of dengue patients.


Keywords


Dengue, IgM ELISA, NS1 Ag ELISA.