International Journal of Agricultural Sciences

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Standardization of Azospirillum Concentration and Duration of Biopriming for Rice Seed Vigour Improvement


Affiliations
1 Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
2 Seed Centre, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
     

   Subscribe/Renew Journal


Azospirillum lipoferum is nitrogen fixing biofertilizer which can be used in seed treatment for improving seedling vigour. In order to standardize the optimum concentration of liquid Azospirillum for rice biopriming seed treatment and duration of biopriming, a laboratory experiment was conducted in Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore in CompletelyRandomizedDesign with four replicates. Rice hybrid CORH 4 and its parental lines COMS 23A and CB 174R seeds were bioprimed separately with 10, 15 and 20 per cent of Azospirillum for 6, 12, 18 and 24h of durations. Unprimed and hydroprimed seeds were used as control. The result revealed that rice seeds bioprimed with 20 per cent Azospirillum for 12 h was found to be the best seed biopriming treatment for both CORH 4 rice hybrid and its parental lines to enhance the germination rate, total germination percentage, seedling growth and vigour. In rice hybrid CORH 4, biopriming with 20 per cent liquid Azospirillum for 12h expressed an increase of 14 per cent of vigour index over the non-primed control seeds.

Keywords

Azospirillum lipoferum, Biopriming, Seed Germination, Seedling Vigour.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Abdul-Baki, A.A. and Anderson, J.D. (1973). Vigour deterioration of soybean seeds by multiple criteria. Crop Sci., 13 : 630-633.

  • Afifi, M.H., Manal, F.M. and Gomaa, A.M. (2003). Efficiency of applying biofertilizers to maize crop under different levels of mineral fertilizers. Ann. Agric. Sci. Moshtohor., 41(4) : 1411-1420.

  • Bharathi, R., Vivekananthan, R., Harish, S., Ramanathan, A. and Samiyappan, R. (2004). Rhizobacteria-based bioformulations for the management of fruit rot infection in chillies. Crop Protec., 23 : 835-843.

  • Callan, N.W., Mathre, D.E. and Miller, J.B. (1991). Field performance of sweet corn seed bioprimed and coated with Pseudomonas fluorescens ab254. Hort. Sci., 26 : 1163-1165.

  • Fayez, M., Emam, N.F. and Makboul, H.F. (1985). The possible use of nitrogen fixing Azospirilum as biofertilizer for wheat plants. Egypt. J. Microbiol., 20(2) : 199-206.

  • Gomathy, M., Thangaraju, M. and Sarathambal, C. (2009). Optimization of liquid phosphobacteria required for maize seeds. Asian J. Bio. Sci., 4(1) : 53-55.

  • ISTA (2009). International rules for seed testing. International Seed Testing Association, Bassersdorf, Switzerland.

  • Kapulnik, Y., Kigel, Y., Okon, Y., Nur, I. and Henis, Y. (1981). Effect of Azospirillum inoculation on some growth parameters and N content of wheat sorghum and panicum. Pl. Soil, 61: 65-70.

  • Maguire, J.D. (1962). Speed of germination - Aid in selection and evaluation of seedling emergence and vigour. Crop Sci., 2 : 176-177.

  • Niranjan, S.R., Shetty, N.P. and Shetty, H.S. (2004). Seed bio-priming with Pseudomonas fluorescens isolates enhances growth of pearl millet plants and induces resistance against downy mildew. J. Pest. Mgmt., 50(1) : 41-48.

  • Raju, N.S., Niranjana, S.R., Janardhana, G.R., Prakash, H.S., Shetty, H.S. and Mathur, S.B. (1999). Improvement of seed quality and field emergence of Fusarium moniliforme infected sorghum seeds using biological agents. J. Sci. Food. Agric.,79: 206-212.

  • Ramamoorthy, K., Natarajan, N. and Lakshmanan, A. (2000). Seed biofortification with Azospirillum spp. for improvement of seedling vigour and productivity in rice (Oryza sativa L.). Seed Sci. & Technol., 28 : 809-815.

  • Rangaswamy, R. (2002). A text book of agricultural statistics. New Age International Pvt., Ltd. NEW DELHI, (INDIA).

  • Singleton, P.W., Keyser, H.H. and Sande, E.S. (2002). Development and evaluation of liquid inoculants. In: Inoculants and nitrogen fixation of legumes in Vietnam. (ed.) D. Herridge, ACIAR Proceedings, pp: 52-66.

  • Taylor, A.G. and Harman, G.E. (1990). Concepts and technologies of selected seed treatments. Phytopathol., 28: 321-339.


Abstract Views: 257

PDF Views: 0




  • Standardization of Azospirillum Concentration and Duration of Biopriming for Rice Seed Vigour Improvement

Abstract Views: 257  |  PDF Views: 0

Authors

M. Kokila
Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore (T.N.), India
M. Bhaskaran
Seed Centre, Tamil Nadu Agricultural University, Coimbatore (T.N.), India

Abstract


Azospirillum lipoferum is nitrogen fixing biofertilizer which can be used in seed treatment for improving seedling vigour. In order to standardize the optimum concentration of liquid Azospirillum for rice biopriming seed treatment and duration of biopriming, a laboratory experiment was conducted in Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore in CompletelyRandomizedDesign with four replicates. Rice hybrid CORH 4 and its parental lines COMS 23A and CB 174R seeds were bioprimed separately with 10, 15 and 20 per cent of Azospirillum for 6, 12, 18 and 24h of durations. Unprimed and hydroprimed seeds were used as control. The result revealed that rice seeds bioprimed with 20 per cent Azospirillum for 12 h was found to be the best seed biopriming treatment for both CORH 4 rice hybrid and its parental lines to enhance the germination rate, total germination percentage, seedling growth and vigour. In rice hybrid CORH 4, biopriming with 20 per cent liquid Azospirillum for 12h expressed an increase of 14 per cent of vigour index over the non-primed control seeds.

Keywords


Azospirillum lipoferum, Biopriming, Seed Germination, Seedling Vigour.

References